{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T11:37:47Z","timestamp":1776771467440,"version":"3.51.2"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T00:00:00Z","timestamp":1772236800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T00:00:00Z","timestamp":1772236800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2023A1515030223"],"award-info":[{"award-number":["2023A1515030223"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81801878"],"award-info":[{"award-number":["81801878"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Cattle are frequently involved in civil disputes and offenses. Currently, the paternity testing of cattle is mainly done by analysis of dinucleotide STR (di\u2010STR) or SNPs. However, the di\u2010STR marker usually produces many stutter artifacts that affect the accurate genotyping, and the polymorphic level of SNPs is not high enough. To overcome these limitations, we developed and validated a novel integrated next\u2010generation sequencing (NGS) panel for cattle. It comprises 33 reported di\u2010STRs and 30 newly identified tetranucleotide STRs (tetra\u2010STRs), the mitochondrial displacement loop (D\u2010loop) region, as well as cytochrome b (\n                    <jats:italic>CYTB<\/jats:italic>\n                    ) and recombination\u2010activating gene 1 (\n                    <jats:italic>RAG1<\/jats:italic>\n                    ). The results showed a high genotyping success rate (99.6% on average) and balanced amplification efficiency across most STR loci. In addition, the panel's average genotyping consistency rate reached 96.4% between blood and hair samples. The total discrimination power and cumulative probability of exclusion for duos of this panel were 1\u20131.8\u00a0\u00d7\u00a010\n                    <jats:sup>\u221213<\/jats:sup>\n                    and 1\u20131.7\u00a0\u00d7\u00a010\n                    <jats:sup>\u22128<\/jats:sup>\n                    , respectively. Application in paternity testing caseworks demonstrates its applicability and reliability for resolving paternity disputes. Overall, this study provided a cattle profiling NGS panel with a substantial advancement over current capillary electrophoresis (CE)\u2010based systems, offering superior accuracy, multiplex capability, and efficiency for forensic genetic testing in cattle.\n                  <\/jats:p>","DOI":"10.1002\/elps.70082","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T17:00:10Z","timestamp":1772989210000},"page":"339-352","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Genetic Profiling for Cattle Parentage Testing via Next\u2010Generation Sequencing"],"prefix":"10.1002","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5731-5471","authenticated-orcid":false,"given":"Jiaojiao","family":"Geng","sequence":"first","affiliation":[{"name":"Faculty of Forensic Medicine, Zhongshan School of Medicine Sun Yat\u2010sen University  Guangzhou China"},{"name":"Guangdong 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Research Center Sun Yat\u2010sen University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2176-6884","authenticated-orcid":false,"given":"Zhiyong","family":"Liu","sequence":"additional","affiliation":[{"name":"Faculty of Forensic Medicine, Zhongshan School of Medicine Sun Yat\u2010sen University  Guangzhou China"},{"name":"Guangdong Province Translational Forensic Medicine Engineering Technology Research Center Sun Yat\u2010sen University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-8703-5373","authenticated-orcid":false,"given":"Chaobo","family":"Bao","sequence":"additional","affiliation":[{"name":"Saihan District Branch of Hohhot Public Security Bureau  Hohhot 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