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This study employed an at\u2010line nanofractionation technique to precisely localized potential NAIs in the extract of\n                    <jats:italic>Humulus lupulus<\/jats:italic>\n                    L. Twelve potential compounds, mainly phloroglucinol derivatives, were screened and identified. Seven of these compounds were subsequently isolated, purified, and structurally elucidated by NMR. Among them, Compound\n                    <jats:bold>5<\/jats:bold>\n                    showed remarkable inhibitory activity against both neuraminidase (NA, IC\n                    <jats:sub>50<\/jats:sub>\n                    \u00a0=\u00a00.8\u00a0\u00b5M) and the H3N2 influenza virus (EC\n                    <jats:sub>50<\/jats:sub>\n                    \u00a0=\u00a03.8\u00a0\u00b5M), confirming its potential as a lead compound for anti\u2010influenza development. Compounds\n                    <jats:bold>4<\/jats:bold>\n                    and\n                    <jats:bold>8<\/jats:bold>\n                    , identified as hitherto uncharacterized phloroglucinol derivatives, also demonstrated significant inhibition against NA and the H3N2 influenza virus. Molecular docking revealed that Compounds\n                    <jats:bold>4<\/jats:bold>\n                    and\n                    <jats:bold>5<\/jats:bold>\n                    exhibited superior binding affinities compared to oseltamivir, with\n                    <jats:bold>4<\/jats:bold>\n                    forming critical hydrogen bonds with acidic residues (Glu119, Glu227, and Asp151) in the \u201c150\u2010cavity,\u201d which significantly enhances its inhibitory potency against NAs.\n                  <\/jats:p>","DOI":"10.1002\/elps.70126","type":"journal-article","created":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T09:34:28Z","timestamp":1782207268000},"page":"840-852","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Novel Phloroglucinol Derivatives as Neuraminidase Inhibitors Identified From\n                    <i>Humulus lupulus<\/i>\n                    L. Extract by At\u2010Line Nanofractionation Platform"],"prefix":"10.1002","volume":"47","author":[{"given":"Zhixu","family":"Chen","sequence":"first","affiliation":[{"name":"Institute of Pharmaceutical Analysis, College of Pharmacy; Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research; International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Ministry of Education (MOE) of China Jinan University  Guangzhou China"},{"name":"Department of Pharmaceutical and Pharmacological Sciences, Pharmaceutical Analysis KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biao","family":"Sun","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Analysis, College of Pharmacy; Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research; International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Ministry of Education (MOE) of China Jinan University  Guangzhou China"},{"name":"State Key Laboratory of Bioactive Molecules and Druggability Assessment, &amp; Guangdong Basic Research Center of Excellence for Natural Bioactive Molecules and Discovery of Innovative Drugs Jinan University Guangzhou China"},{"name":"Department of Pharmacy, Xianning Central Hospital The First Affiliated Hospital of Hubei University of Science and Technology  Xianning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanlin","family":"Zeng","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Analysis, College of Pharmacy; Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research; International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Ministry of Education (MOE) of China Jinan University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyang","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Analysis, College of Pharmacy; Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research; International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Ministry of Education (MOE) of China Jinan University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erwin","family":"Adams","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical and Pharmacological Sciences, Pharmaceutical Analysis KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Analysis, College of Pharmacy; Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research; International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Ministry of Education (MOE) of China Jinan University  Guangzhou China"},{"name":"State Key Laboratory of Bioactive Molecules and Druggability Assessment, &amp; 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Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research; International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Ministry of Education (MOE) of China Jinan University  Guangzhou China"},{"name":"State Key Laboratory of Bioactive Molecules and Druggability Assessment, &amp; Guangdong Basic Research Center of Excellence for Natural Bioactive Molecules and Discovery of Innovative Drugs Jinan University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,23]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.17071"},{"key":"e_1_2_10_3_1","first-page":"487","volume-title":"Seminars in respiratory and critical care medicine","author":"Peteranderl C.","year":"2016"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s12276\u2010021\u201000603\u20100"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473\u20103099(12)70121\u20104"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.2c01258"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2020.04.001"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2023.115410"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1039\/C1NP00053E"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11101\u2010018\u20109581\u20101"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/5919237"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6NP00016A"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2023.463817"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2020.461740"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2022.463693"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2024.464986"},{"issue":"223","key":"e_1_2_10_17_1","first-page":"10","article-title":"Protein Data Bank","volume":"233","author":"Bank P. 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