{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T10:04:34Z","timestamp":1790157874357,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100007449","name":"Takeda Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007449","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Electrophoresis"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Low\u2010input mass spectrometry (MS)\u2010based proteomics is vulnerable to peptide loss during final sample preparation. We evaluated whether ChocoTip desalting and decyl maltose neopentyl glycol (DMNG)\u2010assisted reconstitution improve peptide recovery from HeLa digests corresponding to 10\u00a0ng of protein (\u223c50 cells). ChocoTip reduces irreversible peptide adsorption into mesopores of chromatographic particles during microscale desalting, whereas DMNG is expected to suppress hydrophobic\u2010interaction\u2010mediated nonspecific adsorption to plasticware during redissolution, transfer, and injection. After selecting DMNG as the nonionic detergent additive, we compared conventional StageTip desalting, ChocoTip desalting, DMNG\u2010assisted reconstitution, and their combination. ChocoTip and DMNG method each improved peptide recovery but affected the peptide populations eluting in different regions of the nanoflow reversed\u2010phase liquid chromatography (nanoRPLC) gradient. Their combination yielded the highest number of peptide identifications and increased peptide intensities across almost the entire nanoRPLC gradient, including commonly identified peptides. Peptides uniquely recovered by the combined workflow covered a broad hydrophobicity range and included longer sequences. These results indicate that suppressing peptide loss at both desalting and post\u2010desalting stages provides a simple strategy to improve sensitivity and peptide coverage in low\u2010input MS\u2010based proteomics.<\/jats:p>","DOI":"10.1002\/elps.70161","type":"journal-article","created":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T09:17:57Z","timestamp":1790155077000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Improved Peptide Recovery in Low\u2010Input MS\u2010Based Proteomics Using High\u2010Recovery StageTip Desalting and Nonionic Detergent\u2010Assisted Reconstitution"],"prefix":"10.1002","author":[{"given":"Akane","family":"Omori","sequence":"first","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eisuke","family":"Kanao","sequence":"additional","affiliation":[{"name":"Graduate School 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