{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T19:55:33Z","timestamp":1789415733637,"version":"build-2803163510"},"reference-count":32,"publisher":"Wiley","issue":"7-8","license":[{"start":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T00:00:00Z","timestamp":1743033600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21974015"],"award-info":[{"award-number":["21974015"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>This paper introduces a novel preparation of open\u2010tubular affinity capillary electrochromatography (OT\u2010ACEC) columns through immobilizing proteins by pore entrapment with metal\u2010organic frameworks (MOFs) to rapidly investigate drug\u2013protein interactions. The MOFs material PCN\u2010333(Al) was used to immobilize the model receptor bovine hemoglobin (BHb) for preparing BHb@MOFs, and the affinity chromatography BHb@MOFs\/PDA@capillary was created through the immobilization of BHb@MOFs facilitated by polydopamine (PDA). The disappearance of the mesoporous pore of PCN\u2010333 (Al) proved the encapsulation of BHb within the pore of PCN\u2010333(Al). The BHb@MOFs\/PDA@capillary column exhibited a good affinity screening and separation ability to distinguish flavonoids and sulfonamides. Besides, the normalized capacity factor (<jats:italic>K<\/jats:italic><jats:sub>IRCE<\/jats:sub>) was used to evaluate drug\u2013protein interactions and related to apparent migration times and the amount of immobilized protein. The binding constant (<jats:italic>K<\/jats:italic><jats:sub>a<\/jats:sub>) values was derived from fluorescence quenching analysis, and a robust positive correlation was observed between <jats:italic>K<\/jats:italic><jats:sub>IRCE<\/jats:sub> and lg <jats:italic>K<\/jats:italic><jats:sub>a<\/jats:sub> (<jats:italic>R<\/jats:italic> = 0.94783, <jats:italic>p<\/jats:italic> &lt; 0.00001). These results indicate that <jats:italic>K<\/jats:italic><jats:sub>IRCE<\/jats:sub> can be used as an effective index to evaluate the affinity between protein molecules and drug molecules and the OT\u2010ACEC has the potential to predict drug activity.<\/jats:p>","DOI":"10.1002\/elps.8117","type":"journal-article","created":{"date-parts":[[2025,3,30]],"date-time":"2025-03-30T00:36:37Z","timestamp":1743294997000},"page":"357-364","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Open\u2010Tubular Affinity Capillary Electrochromatography Based on Immobilized Receptor by Pore Entrapment With Metal\u2013Organic Frameworks"],"prefix":"10.1002","volume":"46","author":[{"given":"Meiling","family":"Zhou","sequence":"first","affiliation":[{"name":"Chongqing Key Laboratory of Natural Product Synthesis and Drug Research Innovative Drug Research Center School of Pharmaceutical Sciences Chongqing University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy The Affiliated Hospital of Southwest Medical University  Luzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanwen","family":"Deng","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Natural Product Synthesis and Drug Research Innovative Drug Research Center School of Pharmaceutical Sciences Chongqing University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Chen","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Natural Product Synthesis and Drug Research Innovative Drug Research Center School of Pharmaceutical Sciences Chongqing University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Wang","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Natural Product Synthesis and Drug Research Innovative Drug Research Center School of Pharmaceutical Sciences Chongqing University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yike","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Pharmacy Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhining","family":"Xia","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Natural Product Synthesis and Drug Research Innovative Drug Research Center School of Pharmaceutical Sciences Chongqing University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2022.123515"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2018.12.005"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpclett.3c01002"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2023.122630"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2013.01.061"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.201900013"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.8b04741"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2018.05.006"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.202200191"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202200084"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.2c07560"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.201500414"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.201400583"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2010.02.013"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(03)00056-6"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0153081"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.201800207"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cben.201900016"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.iecr.9b00448"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13205-019-1969-0"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2021.122959"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja2038003"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201811327"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms6979"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacsau.1c00226"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.0c06964"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-02103-0"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00604-021-05065-9"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.0c07870"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf201536v"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2007.10.037"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2021.102641"}],"container-title":["ELECTROPHORESIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.8117","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T18:10:21Z","timestamp":1760551821000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elps.8117"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,27]]},"references-count":32,"journal-issue":{"issue":"7-8","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["10.1002\/elps.8117"],"URL":"https:\/\/doi.org\/10.1002\/elps.8117","archive":["Portico"],"relation":{},"ISSN":["0173-0835","1522-2683"],"issn-type":[{"value":"0173-0835","type":"print"},{"value":"1522-2683","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,27]]},"assertion":[{"value":"2024-11-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}