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Despite the availability of diagnostic tools such as the Prostate Specific Antigen (PSA) test, its low sensitivity reinforces the need for the exploration of more reliable biomarkers. In this context, metabolomics offers a promising approach for identifying sensitive biomarkers to improve cancer diagnosis and treatment. Therefore, this study aimed to conduct a targeted metabolomic analysis of the extracellular environment of In Vitro non\u2010tumoral and cancer prostate cells to compare the levels of eight nucleosides using micellar electrokinetic capillary chromatography with UV detection (MEKC\u2010UV). The method was adapted from a previously optimized protocol for blood serum, with minor adjustments to meet the Brazilian National Health Surveillance Agency (ANVISA) standards. Nucleosides were extracted via solid\u2010phase extraction (SPE), and cell cultures were maintained under controlled conditions at 37\u00b0C with 5% CO<jats:sub>2<\/jats:sub> until reaching 80% confluence. The optimized MEKC\u2010UV method demonstrated precision and accuracy, although the Youden test indicated some lack of robustness. Statistical analysis using a two\u2010tailed <jats:italic>t<\/jats:italic>\u2010test revealed significantly higher adenosine levels in non\u2010tumoral cells, whereas uridine and 5\u2010methyluridine concentrations were elevated in cancer cells. Inosine was detected exclusively in the non\u2010tumoral cell line. Nevertheless, the method's innovative and cost\u2010effective nature underscores its potential as a tool for cancer biomarker identification, with distinct nucleoside patterns in cancer cells offering valuable insights for disease recognition.<\/jats:p>","DOI":"10.1002\/elps.8120","type":"journal-article","created":{"date-parts":[[2025,3,19]],"date-time":"2025-03-19T08:06:17Z","timestamp":1742371577000},"page":"1006-1013","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Modified Nucleosides as Potential Biomarkers of Prostate Cancer: Targeted Metabolomics of In Vitro Cell Samples by MEKC\u2010UV"],"prefix":"10.1002","volume":"46","author":[{"given":"Isabela","family":"Rocha","sequence":"first","affiliation":[{"name":"Laboratory of Analysis of Biomolecules Tiselius Institute of Chemistry Universidade Estadual de Campinas Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingridi Rafaela","family":"de Brito","sequence":"additional","affiliation":[{"name":"Department of Structural and Functional Biology Institute of Biology Universidade Estadual de Campinas Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hernandes F.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Department of Structural and Functional Biology Institute of Biology Universidade Estadual de Campinas Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline Mara","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Department of Structural and Functional Biology Institute of Biology Universidade Estadual de Campinas Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Val\u00e9ria Colnaghi","family":"Simionato","sequence":"additional","affiliation":[{"name":"Laboratory of Analysis of Biomolecules Tiselius Institute of Chemistry Universidade Estadual de Campinas Campinas Brazil"},{"name":"National Institute of Science and Technology for Bioanalytics Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,19]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"The International Agency for Research on Cancer (IARC) Global Cancer Observatory [Internet]. gco.iarc.fr. 2020 [cited 2023 Jul 31] accessed July 31 2023 https:\/\/gco.iarc.who.int\/media\/globocan\/factsheets\/cancers\/27\u2010prostate\u2010fact\u2010sheet."},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0221775"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1559-0275-11-45"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac0614341"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo12020194"},{"key":"e_1_2_9_7_1","volume-title":"Health Promotion and Disease Prevention in Clinical Practice","author":"Woolf S. 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