{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T15:10:23Z","timestamp":1785251423075,"version":"3.55.0"},"reference-count":27,"publisher":"Wiley","issue":"7-8","license":[{"start":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T00:00:00Z","timestamp":1742256000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Electrophoresis"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>9\u2010Methylfascaplysin, a derivative of the marine natural product fascaplysin, has shown promising anti\u2010 Alzheimer's disease (AD) potential through its anti\u2010\u03b2\u2010amyloid (A\u03b2) neuroprotective effects. However, the pharmacokinetics (PK) of 9\u2010methylfascaplysin, crucial for its preclinical evaluation, have not been thoroughly studied. In this study, we developed and validated a sensitive and accurate ultra\u2010performance liquid chromatography\u2010tandem mass spectrometry (UPLC\u2010MS\/MS) method for the quantification of 9\u2010methylfascaplysin in rat plasma. The method demonstrated a lower limit of detection (LLOD) of 1\u00a0ng\/mL and a linear quantification range of 5\u20132000\u00a0ng\/mL. The PK study in rat plasma was conducted. After intragastric administration, the plasma concentration of 9\u2010methylfascaplysin peaked at a maximum concentration (<jats:italic>C<\/jats:italic><jats:sub>max<\/jats:sub>) of 193.4\u00a0ng\/m and an enterohepatic circulation (EHC) phenomenon was observed. By comparing the area under the plasma concentration\u2010time curve (AUC) values obtained from intragastric and intravenous administrations, the absolute oral bioavailability (F) of 9\u2010methylfascaplysin was determined as 18.3%. The tissue distribution study revealed that following a single intragastric administration, 9\u2010methylfascaplysin was most concentrated in the stomach, followed by the small intestine, large intestine, liver, kidney, brain, lung, spleen, and heart in descending order. Furthermore, the excretion profiles of 9\u2010methylfascaplysin in rat urine and feces were studied. The results of this study provide valuable insights into the PK behavior of 9\u2010methylfascaplysin and serve as a foundation for its further preclinical evaluation and potential clinical application as an anti\u2010AD agent.<\/jats:p>","DOI":"10.1002\/elps.8135","type":"journal-article","created":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T07:39:54Z","timestamp":1742283594000},"page":"452-461","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Pharmacokinetics, Tissue Distribution, and Excretion of 9\u2010Methylfascaplysin, a Potential Anti\u2010Alzheimer's Disease Agent"],"prefix":"10.1002","volume":"46","author":[{"given":"Manman","family":"Zhang","sequence":"first","affiliation":[{"name":"Center for Rehabilitation Medicine Department of Neurology Zhejiang Provincial People's Hospital Affiliated People's Hospital Hangzhou Medical College  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Drug Monitoring and Control of Zhejiang Province National Narcotics Laboratory Zhejiang Regional Center  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingjian","family":"Sun","sequence":"additional","affiliation":[{"name":"School of Mathematical Sciences Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolu","family":"Shi","sequence":"additional","affiliation":[{"name":"Key Laboratory of Drug Monitoring and Control of Zhejiang Province National Narcotics Laboratory Zhejiang Regional Center  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongze","family":"Liang","sequence":"additional","affiliation":[{"name":"School of Materials Science and Chemical Engineering Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowei","family":"Chen","sequence":"additional","affiliation":[{"name":"Ningbo Key Laboratory of Behavioral Neuroscience Zhejiang Provincial Key Laboratory of Pathophysiology School of Medicine Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Cui","sequence":"additional","affiliation":[{"name":"Ningbo Key Laboratory of Behavioral Neuroscience Zhejiang Provincial Key Laboratory of Pathophysiology School of Medicine Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yilei","family":"Fan","sequence":"additional","affiliation":[{"name":"The Department of Criminal Science and Technology Zhejiang Police College  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianfeng","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine The Second Affiliated Hospital of Wenzhou Medical University  Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haixing","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Drug Monitoring and Control of Zhejiang Province National Narcotics Laboratory Zhejiang Regional Center  Hangzhou China"},{"name":"Department of Anesthesiology and Perioperative Medicine The Second Affiliated Hospital of Wenzhou Medical University  Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/alz.13016"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)32205-4"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp-2018-318820"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/jo00249a025"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/md20030185"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/md21040226"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/md21080424"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/md17020121"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschemneuro.9b00503"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573-021-00139-y"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/cns.70100"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2021.107656"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/md18110557"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.130725"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.34509"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2019.04.012"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2023.464086"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpha.2023.09.009"},{"key":"e_1_2_8_20_1","unstructured":"\u201cM10 Bioanalytical Method Validation and Study Sample Analysis \u201d U.S. Department of Health and Human Services Food and Drug Administration Center for Drug Evaluation and Research (CDER) Center for Biologics Evaluation and Research (CBER);2022."},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2022.123097"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003088-200241100-00005"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.5b01608"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/ddr.21463"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1080\/00498254.2023.2299686"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2025.108128"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2013.09.007"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2017.28"}],"container-title":["ELECTROPHORESIS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elps.8135","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T18:10:18Z","timestamp":1760551818000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elps.8135"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,18]]},"references-count":27,"journal-issue":{"issue":"7-8","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["10.1002\/elps.8135"],"URL":"https:\/\/doi.org\/10.1002\/elps.8135","archive":["Portico"],"relation":{},"ISSN":["0173-0835","1522-2683"],"issn-type":[{"value":"0173-0835","type":"print"},{"value":"1522-2683","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,18]]},"assertion":[{"value":"2024-12-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}