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In this research, we show that the Oxford Nanopore Technology (ONT) offers a good alternative to study the <jats:italic>MC1R<\/jats:italic> sequence variation. MinION was used to sequence the 1590\u00a0bp <jats:italic>MC1R<\/jats:italic> exon and minimal promoter in a cohort of 126 subjects, including 65 red\u2010haired individuals, using the FLO\u2010MIN106 (R9.4) chemistry. Assigned DNA variants were validated using Ion Torrent technology provided with Ion Xpress Plus Fragment Library Kit and the Personal Genome Machine<jats:sup>TM<\/jats:sup> (PGM<jats:sup>TM<\/jats:sup>). We show that the use of the latest sequencing kit V14 together with the FLO\u2010MIN114 (R10.4.1) flow cell has eliminated the systematic errors observed with the previous chemistry and allowed reliable detection of short indels important for phenotypic inference. Importantly, the use of the algorithm implemented in the EPI2ME software enabled convenient and accurate read\u2010based phase determination which can be useful in data interpretation.<\/jats:p>","DOI":"10.1002\/elps.8141","type":"journal-article","created":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T15:04:16Z","timestamp":1746198256000},"page":"727-731","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Investigation of the <i>MC1R<\/i> Gene Sequence Variation Using Oxford Nanopore Sequencing"],"prefix":"10.1002","volume":"46","author":[{"given":"Wojciech","family":"Branicki","sequence":"first","affiliation":[{"name":"Institute of Zoology and Biomedical Research, Jagiellonian University Krak\u00f3w Poland"},{"name":"Institute of Forensic Research Krak\u00f3w 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