{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T17:21:02Z","timestamp":1787505662523,"version":"build-2736575974"},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,12,21]],"date-time":"2016-12-21T00:00:00Z","timestamp":1482278400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Engineering in Life Sciences"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:p>\n                    Today, crude oil is an important source of energy and environmental contamination due to the continued use of petroleum products is a matter or urgent concern. In this work, two technological platforms, namely, the use of a robust desulfurizing bacteria and the use of nanotechnology to decorate the surface of the bacteria with nanoparticles (NP), were combined to enhance biodesulfurization (BDS). BDS is an ecologically friendly method for desulfurizing petroleum products while avoiding damage to the hydrocarbons due to the high temperatures\u00a0normally associated with physical desulfurization methods.\u00a0First, a bacterium known to be a good organism for desulfurization (\n                    <jats:italic>Rhodococcus erythropolis<\/jats:italic>\n                    IGTS8) was employed in cell culture to remove a recalcitrant sulfur molecule from a common sulfur\u2010containing compound found in crude petroleum products (dibenzothiophene). 2\u2010Hydroxybiphenyl (2\u2010HBP) produced as a consequence of the BDS of dibenzothiophene was determined using Gibbs\u2019 assay. The synthesized NP were characterized by field emission scanning electron microscope, transmission electron microscopy, Fourier transform infrared spectroscopy, X\u2010ray diffraction spectroscopy, and vibrating sample magnetometer. The field emission scanning electron microscope and transmission electron microscopy images showed the size of the NP is 7\u20138 nm. The decorated cells had a long lag phase, but the growth continued until 148 h (at OD\n                    <jats:sub>600<\/jats:sub>\n                    = 3.408) while the noncoated bacteria grow until 96 h before entering the stationary phase at OD\n                    <jats:sub>600<\/jats:sub>\n                    = 2.547. Gibbs\u2019 assay results showed that production of 2\u2010HBP by decorated cells was 0.210 mM at\n                    <jats:italic>t<\/jats:italic>\n                    = 148 h, while 2\u2010HBP production by nondecorated cells was 0.182 mM at\n                    <jats:italic>t<\/jats:italic>\n                    = 96 h. Finally, the experiments were repeated in a fermenter.\n                  <\/jats:p>","DOI":"10.1002\/elsc.201600080","type":"journal-article","created":{"date-parts":[[2016,11,16]],"date-time":"2016-11-16T08:24:47Z","timestamp":1479284687000},"page":"528-535","source":"Crossref","is-referenced-by-count":33,"title":["DBT desulfurization by decorating\n                    <i>Rhodococcus erythropolis<\/i>\n                    IGTS8 using magnetic Fe\n                    <sub>3<\/sub>\n                    O\n                    <sub>4<\/sub>\n                    nanoparticles in a bioreactor"],"prefix":"10.1002","volume":"17","author":[{"given":"Elham","family":"Karimi","sequence":"first","affiliation":[{"name":"Department of Life Science Engineering, Faculty of New Sciences and Technologies University of Tehran  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clayton","family":"Jeffryes","sequence":"additional","affiliation":[{"name":"Dan F. Smith Department of Chemical Engineering Lamar University  Beaumont TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatemeh","family":"Yazdian","sequence":"additional","affiliation":[{"name":"Department of Life Science Engineering, Faculty of New Sciences and Technologies University of Tehran  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abbas","family":"Akhavan Sepahi","sequence":"additional","affiliation":[{"name":"Microbiology and Biotechnology Research Group Islamic Azad University  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ash","family":"Hatamian","sequence":"additional","affiliation":[{"name":"Department of Life Science Engineering, Faculty of New Sciences and Technologies University of Tehran  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Behnam","family":"Rasekh","sequence":"additional","affiliation":[{"name":"Microbiology and Biotechnology Research Group Research Institute of Petroleum Industry  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamid","family":"Rashedi","sequence":"additional","affiliation":[{"name":"School of Chemical Engineering, College of Engineering University of Tehran  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meisam","family":"Omidi","sequence":"additional","affiliation":[{"name":"Protein Research Centre Shahid Beheshti University, GC  Velenjak Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad\u2010Bagher","family":"Ebrahim\u2010Habibi","sequence":"additional","affiliation":[{"name":"Microbiology and Biotechnology Research Group Research Institute of Petroleum Industry  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyed Jamal","family":"Ashrafi","sequence":"additional","affiliation":[{"name":"Department of Life Science Engineering, Faculty of New Sciences and Technologies University of Tehran  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,12,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-004-1755-7"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2008\/017608-0"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2007.08.016"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2007.07.003"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2006.04.005"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fuel.2005.04.004"},{"key":"e_1_2_7_8_1","volume-title":"Dibenzothiophene desulfurization by Gordonia alkanivorans strain 1B","author":"Alves G.","year":"2007"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/00021369.1973.10860640"},{"key":"e_1_2_7_10_1","volume-title":"Chemistry of Diesel Fuels","author":"Song C.","year":"2000"},{"key":"e_1_2_7_11_1","first-page":"213","article-title":"The study of biodesulfurization activity in recombinant E. coli strain by cloning the dsz genes involve in 4S pathway","volume":"22","author":"Raheb J.","year":"2011","journal-title":"J. Sci. Islam. Repub. Iran"},{"key":"e_1_2_7_12_1","first-page":"141","article-title":"The enhancement of biodesulfurization activity in a novel indigenous engineered Pseudomonas putida","volume":"13","author":"Raheb J.","year":"2009","journal-title":"Iran. Biomed. J"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01024736"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-7799(89)90007-3"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0921-3449(90)90046-7"},{"key":"e_1_2_7_16_1","first-page":"523","article-title":"Desulfurization activity and reusability of magnetite nanoparticle\u2013coated Rhodococcus erythropolis FMF and R. erythropolis IGTS8 bacterial cells","volume":"60","author":"Bardania H.","year":"2012","journal-title":"Biotechnol. Appl. 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Effects of biodesulfurization by bacterial cell of crude oil to reduce air pollution and the environment 2nd International Symposium on Environment and Morality ISEM2014 Adiyaman University October 24\u201326 2014."},{"key":"e_1_2_7_25_1","unstructured":"Karimi E. Yazdian F. Rasekh B. Hatamian A. S.et\u00a0al. 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