{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T06:45:33Z","timestamp":1782197133154,"version":"3.54.5"},"reference-count":55,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,11,12]],"date-time":"2018-11-12T00:00:00Z","timestamp":1541980800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007298","name":"Urmia University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007298","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Engineering in Life Sciences"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The use of nanotechnology and biotechnology to improve the production of plant bioactive compounds is growing. <jats:italic>Hyoscyamus reticulatus<\/jats:italic> L. is a major source of tropane alkaloids with a wide therapeutic use, including treatment of Parkinson's disease and to calm schizoid patients. In the present study, hairy roots were obtained from two\u2010week\u2010old cotyledon explants of <jats:italic>H. reticulatus<\/jats:italic> L. using the A7 strain of <jats:italic>Agrobacterium rhizogenes<\/jats:italic>. The effects of different concentrations of the signaling molecule nano\u2010zinc oxide (ZnO) (0, 50, 100 and 200\u00a0mg\/L), with three exposure times (24, 48 and 72 h), on the growth rate, antioxidant enzyme activity, total phenol contents (TPC), tropane alkaloid contents and hyoscyamine\u20106\u2010<jats:italic>beta<\/jats:italic>\u2010hydroxylase (<jats:italic>h6h<\/jats:italic>) gene expression levels were investigated. Growth curve analysis revealed a decrease in fresh and dry weight of ZnO\u2010treated hairy roots compared to the control. ANOVA results showed that the antioxidant activity of the enzymes catalase, guaiacol peroxidase and ascorbate peroxidase was significantly higher in the ZnO\u2010treated hairy roots than in the control, as was the TPC. The highest levels of hyoscyamine (37%) and scopolamine (37.63%) were obtained in hairy roots treated with 100\u00a0mg\/L of ZnO after 48 and 72 h, respectively. Semi\u2010quantitative RT\u2010PCR analysis revealed the highest <jats:italic>h6h<\/jats:italic> gene expression was in hairy roots treated with 100\u00a0mg\/L of ZnO after 24 h. It can be concluded that ZnO is as an effective elicitor of tropane alkaloids such as hyoscyamine and scopolamine due to its enhancing effect on expression levels of the biosynthetic <jats:italic>h6h<\/jats:italic> gene.<\/jats:p>","DOI":"10.1002\/elsc.201800087","type":"journal-article","created":{"date-parts":[[2018,10,26]],"date-time":"2018-10-26T05:59:50Z","timestamp":1540533590000},"page":"73-89","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":79,"title":["Influence of nano\u2010zinc oxide on tropane alkaloid production, <i>h6h<\/i> gene transcription and antioxidant enzyme activity in <i>Hyoscyamus reticulatus<\/i> L. hairy roots"],"prefix":"10.1002","volume":"19","author":[{"given":"Kamal Rashidi","family":"Asl","sequence":"first","affiliation":[{"name":"Department of Horticulture Faculty of Agriculture Urmia University  Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bahman","family":"Hosseini","sequence":"additional","affiliation":[{"name":"Department of Horticulture Faculty of Agriculture Urmia University  Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Sharafi","sequence":"additional","affiliation":[{"name":"Zanjan Applied Pharmacology Research Center Zanjan University of Medical Sciences  Zanjan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Palazon","sequence":"additional","affiliation":[{"name":"Department of Plant Physiology Faculty of Pharmacy University of Barcelona  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,11,12]]},"reference":[{"key":"e_1_2_10_2_1","volume-title":"Bioprocessing of Plant In vitro Systems","author":"Vidal\u2010Limon H."},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1515\/znc-2004-11-1216"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules13081722"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12298-014-0235-5"},{"key":"e_1_2_10_6_1","first-page":"43","article-title":"Effect of elicitation on antioxidant activity and production of tropane alkaloids in Hyoscyamus reticulatus hairy root cultures","volume":"3","author":"Zeynali Z.","year":"2016","journal-title":"Res. 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