{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T18:32:40Z","timestamp":1785177160089,"version":"3.55.0"},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,3,22]],"date-time":"2019-03-22T00:00:00Z","timestamp":1553212800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001655","name":"Deutscher Akademischer Austauschdienst","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001655","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003008","name":"Ministry of Higher Education, Egypt","doi-asserted-by":"publisher","award":["57076387"],"award-info":[{"award-number":["57076387"]}],"id":[{"id":"10.13039\/501100003008","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Engineering in Life Sciences"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Induction of an axenic filamentous\u2010like callus growth from the brown algae <jats:italic>Fucus vesiculosus<\/jats:italic> is described. Different treatments were investigated in various combinations to develop axenic cultures based on identification of surface symbionts <jats:italic>via<\/jats:italic> 18S ribosomal RNA. Moreover, viability was confirmed after such processes by 2,3,5\u2010triphenyl tetrazolium chloride assay that demonstrated an average viability of 29%, relative to nonsterilized explants. After six weeks of a phototrophic cultivation on artificial sea water\u201012\u2010nitrilotriacetic acid (0.5% w\/v agar), a filamentous\u2010like callus growth was observed, which was identified genetically through its mitochondrial DNA after subculturing. Achievement of confirmed marine callus cultures might enrich old previously established blue biotechnology techniques and open new chances for cultivation of brown algae for production of good manufacturing practice\u2010compliant bioproducts.<\/jats:p>","DOI":"10.1002\/elsc.201800211","type":"journal-article","created":{"date-parts":[[2019,3,18]],"date-time":"2019-03-18T13:53:15Z","timestamp":1552917195000},"page":"363-369","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Induction and genetic identification of a callus\u2010like growth developed in the brown alga <i>Fucus vesiculosus<\/i>"],"prefix":"10.1002","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5747-6972","authenticated-orcid":false,"given":"Ahmed","family":"Zayed","sequence":"first","affiliation":[{"name":"Department of Mechanical and Process Engineering Institute of Bioprocess Engineering Technical University of Kaiserslautern  Kaiserslautern Germany"},{"name":"Department of Pharmacognosy College of Pharmacy Tanta University  Tanta Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariya","family":"Kovacheva","sequence":"additional","affiliation":[{"name":"Department of Mechanical and Process Engineering Institute of Bioprocess Engineering Technical University of Kaiserslautern  Kaiserslautern Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Muffler","sequence":"additional","affiliation":[{"name":"Department of Life Sciences and Engineering University of Applied Sciences Bingen  Bingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans\u2010Werner","family":"Breiner","sequence":"additional","affiliation":[{"name":"Department of Biology Institute of Ecology Technical University of Kaiserslautern  Kaiserslautern Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thorsten","family":"Stoeck","sequence":"additional","affiliation":[{"name":"Department of Biology Institute of Ecology Technical University of Kaiserslautern  Kaiserslautern Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7674-0967","authenticated-orcid":false,"given":"Roland","family":"Ulber","sequence":"additional","affiliation":[{"name":"Department of Mechanical and Process Engineering Institute of Bioprocess Engineering Technical University of Kaiserslautern  Kaiserslautern Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,3,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10811-007-9205-4"},{"key":"e_1_2_8_3_1","unstructured":"Se\u2010kwon K. Venkatesan J. Handbook of Marine Biotechnology Springer\u2010Verlag Berlin Heidelberg 2015."},{"key":"e_1_2_8_4_1","first-page":"183","article-title":"A simple method of seaweed axenic culture","volume":"4","author":"Masakazu T.","year":"1989","journal-title":"Korean J. Phycol."},{"key":"e_1_2_8_5_1","first-page":"91","article-title":"Sea weed biotechnology","volume":"20","author":"Kaliaperfumal N.","year":"1998","journal-title":"Proc. Firts Nayl. Semi Mar. Biotech."},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsps.2009.12.001"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2012.06.016"},{"key":"e_1_2_8_8_1","unstructured":"Hurst D. B\u00f8rresen T. Almesj\u00f6 L. DeRaedemaecker F.et\u00a0al. Marine biotechnology strategic research and innovation roadmap: insights to the future direction of European marine biotechnology 2016."},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02843827"},{"key":"e_1_2_8_10_1","first-page":"1","article-title":"Microbes in the marine environment","author":"Munn C.","year":"2011","journal-title":"Mar. Microbiol. Ecol. Appl."},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1131855100"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1515\/bot-2017-0017"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1077075"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/md15010014"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.2216\/i0031-8884-24-4-481.1"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-90-481-3795-4_17"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.2216\/i0031-8884-43-5-596.1"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781119977087"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1835.2008.00520.x"},{"key":"e_1_2_8_20_1","unstructured":"Qiagen AllPrep DNA \/ RNA mini handbook for simultaneous purification of genomic DNA and total RNA from the same animal.2005 1\u201356."},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.06941-11"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1008059314639"},{"key":"e_1_2_8_23_1","first-page":"727","article-title":"Induction of callus from the marine brown alga Dictyosiphon foeniculaceus","volume":"23","author":"Saga N.","year":"1982","journal-title":"Plant Cell Physiol"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1835.1997.tb00069.x"},{"key":"e_1_2_8_25_1","unstructured":"Andersen R. A.(Ed.) Algal Culturing Techniques Elsevier Academic Press MA2005."},{"key":"e_1_2_8_26_1","volume-title":"DNeasy Plant Handbook","author":"QIAGEN","year":"2018"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2006.01.019"},{"key":"e_1_2_8_28_1","unstructured":"New England Biolabs DNA MODIFYING ENZYMES NEB: PCR Cloning Kit Kit Components.2017 4."},{"key":"e_1_2_8_29_1","first-page":"133","volume-title":"Algal Culturing Techniques","author":"Kawai H.","year":"2005"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.4319\/lom.2011.9.478"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1529-8817.2009.00708.x"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejop.2004.07.003"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.4141\/cjas78-064"},{"key":"e_1_2_8_34_1","first-page":"207","article-title":"2, 3, 5\u2010Triphenyltetrazolium reduction in the viability assay of Ulva fasciata (Chlorophyta) in response to salinity stress","volume":"40","author":"Chang W.","year":"1999","journal-title":"Bot. Bull."},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00306103"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaeng.2004.03.007"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/bp020123i"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10811-006-9104-0"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2019.01.105"}],"container-title":["Engineering in Life Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Felsc.201800211","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elsc.201800211","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/elsc.201800211","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/elsc.201800211","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T15:27:36Z","timestamp":1760542056000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/elsc.201800211"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,22]]},"references-count":38,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2019,5]]}},"alternative-id":["10.1002\/elsc.201800211"],"URL":"https:\/\/doi.org\/10.1002\/elsc.201800211","archive":["Portico"],"relation":{},"ISSN":["1618-0240","1618-2863"],"issn-type":[{"value":"1618-0240","type":"print"},{"value":"1618-2863","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,22]]},"assertion":[{"value":"2018-12-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}