{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T18:42:06Z","timestamp":1760553726047,"version":"build-2065373602"},"reference-count":26,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,10,13]],"date-time":"2020-10-13T00:00:00Z","timestamp":1602547200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Engineering in Life Sciences"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study aimed to investigate the expression of B\u2010cell lymphoma\u2010extra large (Bcl\u2010xL) in cartilage tissues following articular cartilage injury and to determine its effects on the biological function of chondrocytes. A total of 25 necrotic cartilage tissue samples and 25 normal tissue samples were collected from patients diagnosed with osteoarthritis at our hospital from December 2015 to December 2018. The mRNA expression levels of Bcl\u2010xL, caspase\u20103, and matrix metalloproteinase\u20103 (MMP\u20103) in the normal and necrotic tissues were examined via quantitative polymerase chain reaction, and their protein expression levels were detected via western blotting. The expression levels of Bcl\u2010xL, insulin\u2010like growth factor\u20101 (IGF\u20101), and bone morphogenetic protein (BMP) were significantly lower but those of caspase\u20103, MMP\u20103, interleukin\u20101\u03b2 (IL\u20101\u03b2), and chemokine\u2010like factor 1 (CKLF1) levels were markedly higher in necrotic cartilage tissues than in normal tissues. Following cell transfection, the expression levels of Bcl\u2010xL, IGF\u20101, and BMP were remarkably higher but those of caspase\u20103, MMP\u20103, IL\u20101\u03b2, and CKLF1 were notably lower in the Si\u2010Bcl\u2010xL group than in the NC group. The Si\u2010Bcl\u2010xL group showed significantly lower cell growth and noticeably higher apoptosis rate than the NC group (normal control group). The expression of Bcl\u2010xL is reduced following articular cartilage injury, and this reduction promotes the proliferation and inhibits the apoptosis of chondrocytes. Therefore, Bcl\u2010xL could serve as a relevant molecular target in the clinical practice of osteoarthritis and other diseases causing cartilage damage.<\/jats:p>","DOI":"10.1002\/elsc.202000039","type":"journal-article","created":{"date-parts":[[2020,10,14]],"date-time":"2020-10-14T01:38:23Z","timestamp":1602639503000},"page":"571-579","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Bcl\u2010xL expression following articular cartilage injury and its effects on the biological function of chondrocytes"],"prefix":"10.1002","volume":"20","author":[{"given":"Zhengjun","family":"Pan","sequence":"first","affiliation":[{"name":"Orthopedics Department The First Affiliated Hospital of Anhui Medical University  Hefei Anhui P. R. China"},{"name":"Orthopedics Department The First People's Hospital of Hefei  Hefei Anhui P. R. 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