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However, the application of 3D\u2010printed parts in these fields requires not only their biocompatibility but also their sterility. The most common method for sterilizing 3D\u2010printed parts is heat steam sterilization\u2014but most commercially available 3D printing materials cannot withstand high temperatures. In this study, a novel heat\u2010resistant polyacrylate material for high\u2010resolution 3D Multijet printing was evaluated for the first time for its resistance to heat steam sterilization and in vitro biocompatibility with mouse fibroblasts (L929), human embryonic kidney cells (HEK 293E), and yeast (<jats:italic>Saccharomyces cerevisiae<\/jats:italic> (<jats:italic>S.\u00a0cerevisiae<\/jats:italic>)). Analysis of the growth and viability of L929 cells and the growth of <jats:italic>S.\u00a0cerevisiae<\/jats:italic> confirmed that the extraction media obtained from 3D\u2010printed parts had no negative effect on the aforementioned cell types, while, in contrast, viability and growth of HEK 293E cells were affected. No different effects of the material on the cells were found when comparing heat steam sterilization and disinfection with ethanol (70%, v\/v). In principle, the investigated material shows great potential for high\u2010resolution 3D printing of novel cell culture systems that are highly complex in design, customized and easily sterilizable\u2014however, the biocompatibility of the material for other cell types needs to be re\u2010evaluated.<\/jats:p>","DOI":"10.1002\/elsc.202100104","type":"journal-article","created":{"date-parts":[[2022,3,31]],"date-time":"2022-03-31T07:41:09Z","timestamp":1648712469000},"page":"699-708","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["<i>In<\/i><i>vitro<\/i> biocompatibility evaluation of a heat\u2010resistant 3D printing material for use in customized cell culture devices"],"prefix":"10.1002","volume":"22","author":[{"given":"Steffen","family":"Winkler","sequence":"first","affiliation":[{"name":"Institute of Technical Chemistry Leibniz University Hannover  Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1198-6664","authenticated-orcid":false,"given":"Katharina V.","family":"Meyer","sequence":"additional","affiliation":[{"name":"Institute of Technical Chemistry Leibniz University Hannover  Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3694-0008","authenticated-orcid":false,"given":"Christopher","family":"Heuer","sequence":"additional","affiliation":[{"name":"Institute of Technical Chemistry Leibniz University Hannover  Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlotta","family":"Kortmann","sequence":"additional","affiliation":[{"name":"Institute of Technical Chemistry Leibniz University Hannover  Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michaela","family":"Dehne","sequence":"additional","affiliation":[{"name":"Institute of Technical Chemistry Leibniz University Hannover  Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janina","family":"Bahnemann","sequence":"additional","affiliation":[{"name":"Institute of Technical Chemistry Leibniz University Hannover  Hannover Germany"},{"name":"Cell Culture Technology Faculty of Technology Bielefeld University  Bielefeld 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