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An electrochemical nanocomposite\u2010based aptasensor is fabricated for the determination of miRNA\u2010128 concentration as the acute lymphoblastic leukemia (ALL) biomarker for the first time. The aptamer chains were immobilized on the surface of the glassy carbon electrode (GCE) through gold nanoparticles\/magnetite\/reduced graphene oxide (AuNPs\/Fe<jats:sub>3<\/jats:sub>O<jats:sub>4<\/jats:sub>\/RGO). Fast Fourier transform infrared (FTIR), X\u2010ray diffraction (XRD), vibrating sample magnetometer (VSM), and transmission electron microscopy (TEM) were used to characterize synthesized nanomaterials. Cyclic voltammetry (CV), square wave voltammetry (SWV), and electrochemical impedance spectroscopy (EIS) were used to characterize the modified GCE in both label\u2010free and labeled methods. The results indicate that the modified working electrode has high selectivity and for miRNA\u2010128 over other biomolecules. The hexacyanoferrate redox system typically operated at around 0.3 V (vs. Ag\/AgCl), and the methylene blue redox system ran at about 0 V, were used as an electrochemical probe. The detection limit and linear detection range for hexacyanoferrate and methylene blue are 0.05346 fM, 0.1\u20130.9 fM, and 0.005483 fM, 0.01\u20130.09 fM, respectively. The stability and diffusion control analyses were performed as well. In both label\u2010free and labeled methods, the modified electron showed high selectivity for miRNA\u2010128. The use of methylene blue as a safer redox mediator caused miRNA\u2010128 to be detected with greater accuracy at low potentials in PBS media. The findings also show the substantial improvement in detection limit and linearity by using reduced graphene oxide\u2010magnetite\u2010gold nanoparticles that can be verified by comparing with previous studies on the detection of other miRNAs.<\/jats:p>","DOI":"10.1002\/elsc.202100170","type":"journal-article","created":{"date-parts":[[2022,5,17]],"date-time":"2022-05-17T08:03:14Z","timestamp":1652774594000},"page":"519-534","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Fabrication of Au\/Fe<sub>3<\/sub>O<sub>4<\/sub>\/RGO based aptasensor for measurement of miRNA\u2010128, a biomarker for acute lymphoblastic leukemia (ALL)"],"prefix":"10.1002","volume":"22","author":[{"given":"Homayoon Soleimani","family":"Dinani","sequence":"first","affiliation":[{"name":"School of Chemical Engineering College of Engineering University of Tehran  Tehran 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