{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T04:52:32Z","timestamp":1793335952815,"version":"4.5.0"},"reference-count":48,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T00:00:00Z","timestamp":1736121600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["469188416"],"award-info":[{"award-number":["469188416"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Engineering in Life Sciences"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The synthesis of enantiopure \u03b1\u2010hydroxy ketones, particularly\n                    <jats:italic>R<\/jats:italic>\n                    \u2010 and\n                    <jats:italic>S<\/jats:italic>\n                    \u2010phenylacetylcarbinol (PAC), represents an important process in the pharmaceutical industry, serving as a pivotal step in the production of drugs. Recently, two novel enzymes, ephedrine dehydrogenase (EDH) and pseudoephedrine dehydrogenase (PseDH), have been described. These enzymes enable the specific reduction of 1\u2010phenyl\u20101,2\u2010propanedione (PPD) to\n                    <jats:italic>R<\/jats:italic>\n                    \u2010PAC and\n                    <jats:italic>S<\/jats:italic>\n                    \u2010PAC, respectively. In this study, we transferred these enzymes into Pickering emulsions, which is an attractive reaction set\u2010up for large\u2010scale synthesis. The bioactive w\/o Pickering emulsion (bioactive Pickering emulsion [BioPE]), in which methyl\n                    <jats:italic>tert<\/jats:italic>\n                    \u2010butyl ether served as the continuous phase, was stabilized by silica nanoparticles. Formate dehydrogenase from\n                    <jats:italic>Rhodococcus jostii<\/jats:italic>\n                    was utilized for cofactor regeneration. Given the considerable complexity of the BioPE, this reaction system underwent a first\u2010time application of design of experiment (DOE) for systematic engineering. A definitive screening design was employed to identify significant factors affecting space\u2010time yield (STY) and conversion. Response surface methodology was used to optimize the conditions, resulting in the observation of a high STY of 4.2 g\u00a0L\u207b\u00b9\u00a0h\u207b\u00b9 and a conversion of 83.2% for BioPE with EDH, and an STY of 4.4 g\u00a0L\u207b\u00b9\u00a0h\u207b\u00b9 and a conversion of 64.5% for BioPE with PseDH.\n                  <\/jats:p>","DOI":"10.1002\/elsc.202400069","type":"journal-article","created":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T03:39:52Z","timestamp":1736134792000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Reaction Engineering for Asymmetric\n                    <i>R<\/i>\n                    \u2010\/\n                    <i>S<\/i>\n                    \u2010PAC Synthesis With Ephedrine or Pseudoephedrine Dehydrogenase in Pickering Emulsion"],"prefix":"10.1002","volume":"25","author":[{"given":"Reynaldo Jr.","family":"Carubio","sequence":"first","affiliation":[{"name":"Chair of Molecular Biotechnology Dresden University of Technology  Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bao\u2010Hsiang","family":"Wang","sequence":"additional","affiliation":[{"name":"Chair of Molecular Biotechnology Dresden University of Technology  Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2912-546X","authenticated-orcid":false,"given":"Marion B.","family":"Ansorge\u2010Schumacher","sequence":"additional","affiliation":[{"name":"Chair of Molecular Biotechnology Dresden University of Technology  Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,1,6]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/ar900196n"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.1337"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1351\/pac199769112371"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260340708"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-018-8945-1"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567-1364.2006.00138.x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6GC01803C"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-002-1084-7"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12257-008-0030-7"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444907050433"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.5b01031"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/slct.201900740"},{"key":"e_1_2_9_14_1","article-title":"Biodegradation of Ephedrine Isomers by Arthrobacter sp. 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