{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T11:40:42Z","timestamp":1767181242697,"version":"3.48.0"},"reference-count":22,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T00:00:00Z","timestamp":1767052800000},"content-version":"vor","delay-in-days":29,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100021828","name":"German Academic Exchange Service","doi-asserted-by":"publisher","award":["57628351"],"award-info":[{"award-number":["57628351"]}],"id":[{"id":"10.13039\/100021828","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Engineering in Life Sciences"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Combining the volumetric fabrication of hydrogel constructs using extrusion bioprinting with highly precise drop\u2010on\u2010demand (DoD) bioprinting offers exciting opportunities in biofabrication. This technical report presents a technique in which a solenoid micro\u2010pipette is operated as an additional tool in an extrusion (bio)printing system to deposit small volumes of bioinks into extrusion\u2010printed hydrogel constructs. Using three exemplary approaches, we show that this enables the patterned placement of cells or growth factors within 3D constructs and thus influences developmental processes. Human cells within low\u2010viscosity bioinks, deposited into extrusion\u2010printed hydrogel constructs by filling inter\u2010strand cavities or by injection into the hydrogel strands, maintained their viability and functionality up to 28 days. As demonstrated for salivary gland cells, the properties of the hydrogel matrix can influence the fate of the injected cells: In a stiff alginate (Alg)\u2010based hydrogel, they formed aggregates, which is beneficial for organoid formation, and in softer hydrogels, they migrated to neighboring cell clusters. Locally injected signaling factors such as vascular endothelial growth factor (VEGF) attracted endothelial cells and fibroblasts, which migrated into previously cell\u2010free hydrogel areas. The combination of extrusion and DoD bioprinting opens new approaches to integrate different cell types and functionalizations in one construct, facilitating the creation of more complex and dynamic models.<\/jats:p>","DOI":"10.1002\/elsc.70062","type":"journal-article","created":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T06:26:06Z","timestamp":1767162366000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Combination of Extrusion and Drop\u2010on\u2010Demand Bioprinting in One Process Enables the Local Placement of Cells or Signaling Factors Into (Bio) Printed Hydrogel Structures"],"prefix":"10.1002","volume":"25","author":[{"given":"Finn","family":"Dani","sequence":"first","affiliation":[{"name":"Centre for Translational Bone Joint and Soft Tissue Research Faculty of Medicine Technische Universit\u00e4t Dresden  Dresden 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Technische Universit\u00e4t Dresden  Dresden Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,30]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43586\u2010021\u201000073\u20108"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201302042"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.0c00008"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1088\/1758\u20105090\/aa71c8"},{"key":"e_1_2_8_6_1","doi-asserted-by":"crossref","unstructured":"F.Koch M.Wehrle K.Trondle et\u00a0al. \u201cRapid Assessment of Combined Drop on Demand and Extrusion\u2010Based Bioprinting With Controlled Shear Stress and High Shape Fidelity \u201d2019 20th International Conference on Solid\u2010State Sensors Actuators and Microsystems & Eurosensors XXXIII (Transducers & Eurosensors XXXIII)( 2019) 1048\u20131051 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