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This study investigates formate utilization for ectoine biosynthesis by the halophilic methylotroph\n                    <jats:italic>Methyloligella halotolerans<\/jats:italic>\n                    . Preliminary growth assays confirmed formate utilization using 15\u201320\u00a0mM formate as the sole energy source substrate, when supplemented with yeast extract or vitamin solutions in a mineral salt medium. In a systematic study for ectoine production, formate utilization reached 0.305\u00a0\u00b1\u00a00.020\u00a0mmol\u00a0d\n                    <jats:sup>\u22121<\/jats:sup>\n                    at 20\u00a0mM. With different C\n                    <jats:sub>1<\/jats:sub>\n                    \u2010substrates at 20\u00a0mM (3\u00a0mmol), ectoine production reached 10.3\u00a0\u00b1\u00a03.2\u00a0\u00b5mol (from methanol), 6.5\u00a0\u00b1\u00a00.8\u00a0\u00b5mol (from equimolar methanol\/formate mix), 4.4\u00a0\u00b1\u00a00.1\u00a0\u00b5mol (from formate), and 1.2\u00a0\u00b1\u00a00.1\u00a0\u00b5mol (from e\u2010formate). Medium buffering, pH stability and toxicity limited performance when formate and e\u2010formate were supplied. Although ectoine yields were suboptimal, the feasibility of e\u2010formate\u2010based ectoine biosynthesis under high\u2010salinity conditions with 9% NaCl, as shown in this study, discloses the great potential for integrating highly efficient electrochemical CO\n                    <jats:sub>2<\/jats:sub>\n                    reduction in saline media with microbial synthesis of organic chemicals.\n                  <\/jats:p>","DOI":"10.1002\/elsc.70063","type":"journal-article","created":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T10:51:23Z","timestamp":1767955883000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Exploring Ectoine Production From Methanol, Formate, and Electrochemically Produced Formate by\n                    <i>Methyloligella halotolerans<\/i>"],"prefix":"10.1002","volume":"26","author":[{"given":"Aykut","family":"Kas","sequence":"first","affiliation":[{"name":"Department of Microbial Biotechnology Helmholtz\u2010Centre For Environmental Research\u2014UFZ  Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paniz","family":"Izadi","sequence":"additional","affiliation":[{"name":"Department of Microbial Biotechnology Helmholtz\u2010Centre For Environmental Research\u2014UFZ  Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudius","family":"Lenz","sequence":"additional","affiliation":[{"name":"Department of Microbial Biotechnology Helmholtz\u2010Centre For Environmental Research\u2014UFZ  Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thore","family":"Rohwerder","sequence":"additional","affiliation":[{"name":"Department of Microbial Biotechnology Helmholtz\u2010Centre For Environmental Research\u2014UFZ  Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens Olaf","family":"Kr\u00f6mer","sequence":"additional","affiliation":[{"name":"Department of Microbial Biotechnology Helmholtz\u2010Centre For Environmental Research\u2014UFZ  Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Falk","family":"Harnisch","sequence":"additional","affiliation":[{"name":"Department of Microbial Biotechnology Helmholtz\u2010Centre For Environmental Research\u2014UFZ  Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010019\u20101681\u20106"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nanoen.2017.08.049"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/celc.202400512"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/celc.202100438"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.7b00086"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2023.1091899"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cssc.202001235"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cssc.202301721"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cssc.202001272"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010018\u201023924\u2010z"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41522\u2010020\u201000151\u2010x"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apenergy.2020.116310"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287\u2010119\u20102\u2010535"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2020.02.010"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcou.2025.103268"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2012.12.001"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2010.06.005"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12934\u2010021\u201001567\u20106"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsenergylett.3c00489"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.01189\u201016"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201711727"},{"key":"e_1_2_9_23_1","first-page":"392","article-title":"Optimization of Growth Medium and Poly\u2010\u03b2\u2010Hydroxybutyric Acid Production From Methanol in Methylobacterium Organophilum","volume":"17","author":"Choi J. 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