{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T20:10:00Z","timestamp":1792872600753,"version":"4.5.0"},"reference-count":59,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T00:00:00Z","timestamp":1775520000000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Engineering in Life Sciences"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The lipase\u2010catalyzed oxidative functionalization of alkenes in Pickering emulsions (PE) offers a green and efficient alternative to conventional processes involving hazardous oxidants. Other investigated reaction media used for this alternative green pathway still suffer from enzyme deactivation and \/or low specific reaction rate which limits their industrial adaptability. This study investigates the enzymatic synthesis of peroxyacetic acid, the first step in lipase\u2010catalyzed oxidative functionalization, in a continuous membrane reactor. The effects of aqueous phase composition, modified silica nanoparticles, and operational parameters on reaction performance were systematically studied. Optimal conditions were obtained at pH 7, 100\u00a0mM buffer, and 5\u00a0g\u00a0L\n                    <jats:sub>dp<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    <jats:sup>1<\/jats:sup>\n                    enzyme concentration. Surface\u2010modified silica nanoparticles improved PE stability and interfacial catalytic efficiency, while maintaining comparable catalytic productivity. Hydrogen peroxide outperformed urea hydrogen peroxide, yielding a maximum product yield of 83% at a concentration of 45\u00a0mM in the influent solution and a space\u2010time yield of 44.9\u00a0g\n                    <jats:sub>PA<\/jats:sub>\n                    L\n                    <jats:sup>\u2212<\/jats:sup>\n                    <jats:sup>1<\/jats:sup>\n                    d\n                    <jats:sup>\u2212<\/jats:sup>\n                    <jats:sup>1<\/jats:sup>\n                    at 386\u00a0mM. At 386\u00a0mM, the specific reaction rate (17.5\u00a0mmol\u00a0g\n                    <jats:sup>\u2212<\/jats:sup>\n                    <jats:sup>1<\/jats:sup>\n                    \u00a0h\n                    <jats:sup>\u2212<\/jats:sup>\n                    <jats:sup>1<\/jats:sup>\n                    ) was over twice that of reported single organic\u2010phase systems. Despite the high peroxide concentrations, the enzyme displayed remarkable stability in PE due to the protective role of nanoparticles. This work provides critical insights into optimizing enzymatic oxidative functionalization in PE and their potential for sustainable industrial applications.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Practical application:<\/jats:italic>\n                    This study provides a foundation for the development of sustainable and efficient continuous processes for oxidative biotransformations using Pickering emulsions (PE). By enabling the enzymatic production of peracids, the first step in lipase\u2010catalyzed oxidative functionalization, the process addresses key limitations of previously explored green reaction systems, such as enzyme deactivation and low specific activity. The PE system enhances emulsion stability and preserves enzyme activity under high oxidant concentrations, achieving high yields and space\u2010time productivity in a membrane\u2010based continuous reactor. Surface\u2010modified silica nanoparticles further improve interfacial catalytic efficiency as well as emulsion stability. This approach is well\u2010suited for selective and eco\u2010friendly oxidation in the synthesis of fine chemicals, active pharmaceutical ingredients, and specialty materials. Additionally, the robustness of the system allows stable operation under harsh conditions, supporting the efficient integration of the second, chemical epoxidation step. These findings contribute to the broader implementation of continuous green chemistry technologies in industrial biocatalysis.\n                  <\/jats:p>","DOI":"10.1002\/elsc.70077","type":"journal-article","created":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T10:16:54Z","timestamp":1775557014000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Continuous Enzymatic Peracids Synthesis in Pickering Emulsions: Influence of Nanoparticles Modification, Aqueous Phase Composition and Operational Parameters"],"prefix":"10.1002","volume":"26","author":[{"given":"Lemuel Onoriode","family":"Adomi","sequence":"first","affiliation":[{"name":"School of Life Science Engineering HTW Berlin \u2013 University of Applied Sciences  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara Fatima","family":"Bhutta","sequence":"additional","affiliation":[{"name":"Institute of Microbiology Technische Chair of Molecular Biotechnology Universit\u00e4t Dresden  Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marion B.","family":"Ansorge\u2010Schumacher","sequence":"additional","affiliation":[{"name":"Institute of Microbiology Technische Chair of Molecular Biotechnology Universit\u00e4t Dresden  Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anja","family":"Drews","sequence":"additional","affiliation":[{"name":"School of Life Science Engineering HTW Berlin \u2013 University of Applied Sciences  Berlin 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