{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T15:11:40Z","timestamp":1764688300194},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,8,26]],"date-time":"2002-08-26T00:00:00Z","timestamp":1030320000000},"content-version":"vor","delay-in-days":237,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Environ and Mol Mutagen"],"published-print":{"date-parts":[[2002,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The mutagen sensitivity assay is one of the approaches used to investigate individual DNA repair capacity. This method is based on the premise that after in vitro treatment with a test mutagen, DNA from subjects with defective repair will be more damaged than DNA from those with an efficient repair system. However, very little is known about unmeasured processes that occur between cell treatment and final assessment of DNA damage. To develop a more precise assay, we modified the traditional mutagen sensitivity assay to also include measurement of DNA damage after culturing cells in the absence of mutagen. First, we treated apparently normal and xeroderma pigmentosum lymphoblastoid cell lines with various doses of benzo(a)pyrene diol epoxide (BPDE) and harvested cells at different time points. A polyclonal antiserum against BPDE\u2010DNA was used to quantitate levels of adducts by immunoslot\u2010blot and immunohistochemistry. Selected conditions included treatment with 10 \u03bcM BPDE, a 4\u2010hr culture in mutagen\u2010free medium, and immunohistochemical measurement of BPDE\u2010DNA adducts. The method was then applied in a pilot study to 50 lymphoblastoid lines from sisters discordant for breast cancer. There was no significant difference between cases and controls in the level of BPDE\u2010DNA adducts in lymphoblasts harvested immediately after BPDE treatment. However, after a 4\u2010hr culture in mutagen\u2010free medium, the level of adducts was significantly higher (<jats:italic>P<\/jats:italic> = 0.006) among cases than in controls. There was a two\u2010fold increase in mean adduct removal in lines from nonaffected as compared to affected sisters (44% and 22% decrease, respectively). DNA repair capacity was predictive of case status (<jats:italic>P<\/jats:italic> = 0.04) in logistic regression analysis. This method, which can be easily applied to large numbers of samples, should be useful in studies to investigate the role of DNA repair in cancer risk. Environ. Mol. Mutagen. 40:93\u2013100, 2002. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/em.10095","type":"journal-article","created":{"date-parts":[[2002,9,5]],"date-time":"2002-09-05T19:39:45Z","timestamp":1031254785000},"page":"93-100","source":"Crossref","is-referenced-by-count":18,"title":["Removal of benzo(a)pyrene diol epoxide (BPDE)\u2010DNA adducts as a measure of DNA repair capacity in lymphoblastoid cell lines from sisters discordant for breast cancer"],"prefix":"10.1002","volume":"40","author":[{"given":"Grazyna","family":"Motykiewicz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatrice","family":"Faraglia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lian Wen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary 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