{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T12:47:41Z","timestamp":1761310061648},"reference-count":44,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2002,9,27]],"date-time":"2002-09-27T00:00:00Z","timestamp":1033084800000},"content-version":"vor","delay-in-days":269,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Environ and Mol Mutagen"],"published-print":{"date-parts":[[2002,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In order to determine if patients with a history of previous urothelial cell carcinoma (UCC) but with current normal urinary cytology have DNA damage in urothelial cells, the single\u2010cell gel electrophoresis (comet) assay was conducted with cells obtained by urinary bladder washings from 44 patients (28 with a history of previous UCC). Increased DNA damage was observed in cytologically \u201cnormal\u201d urothelial cells of patients with a history of UCC when compared with referents with no similar history and after correcting the data for smoking status and age (<jats:italic>P<\/jats:italic> &lt; 0.018). Increased DNA damage also correlated with the highest tumor grade, irrespective of time or course of the disease after clinical intervention (Kendall tau correlation, 0.37, <jats:italic>P<\/jats:italic> = 0.016). Moreover, aneuploidy, as assessed by DNA content ratio (DCR; 75th\/25th percentile of total DNA fluorescence of 50 comets\/patient) was unaltered by smoking status, but increased with UCC grade: 1.39 \u00b1 0.12 (median \u00b1 95% confidence interval; referents); 1.43 \u00b1 0.11 (Grade I UCC; <jats:italic>P<\/jats:italic> = 0.264, against referents); 1.49 \u00b1 0.16 (Grade II UCC; <jats:italic>P<\/jats:italic> = 0.057); 1.57 \u00b1 0.16 (Grade III UCC; <jats:italic>P<\/jats:italic> = 0.003). Micronucleated urothelial cells (MNC) were also scored on Giemsa\u2010stained routine cytological smears and were found not to correlate with DNA damage or DCR. MNC frequencies were higher for patients with a history of UCC and\/or smoking than referents with neither history, but there was no statistical difference between groups. Taken together, these results suggest that the normal\u2010appearing urothelium of patients resected for UCC still harbor genetically unstable cells. Environ. Mol. Mutagen. 40:190\u2013199, 2002. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/em.10109","type":"journal-article","created":{"date-parts":[[2002,10,9]],"date-time":"2002-10-09T04:47:32Z","timestamp":1034138852000},"page":"190-199","source":"Crossref","is-referenced-by-count":25,"title":["DNA damage in cytologically normal urothelial cells of patients with a history of urothelial cell carcinoma"],"prefix":"10.1002","volume":"40","author":[{"given":"\u00c1lisson Marques","family":"de Miranda Cabral Gontijo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o Paulo","family":"de Castro Marcondes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fl\u00e1via","family":"Nunes Elias","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Luiza","family":"Cotrim Sartor de 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