{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T23:51:49Z","timestamp":1773791509462,"version":"3.50.1"},"reference-count":23,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2003,6,5]],"date-time":"2003-06-05T00:00:00Z","timestamp":1054771200000},"content-version":"vor","delay-in-days":155,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"CONACYT","award":["30950M"],"award-info":[{"award-number":["30950M"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Environ and Mol Mutagen"],"published-print":{"date-parts":[[2003,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Most cancers arise in epithelial tissues and these tissues are typically the targets of the carcinogens responsible for the tumors. Exfoliated epithelial cells have traditionally been used for cancer screening by cytopathologists and these cells also can be used for biomonitoring of genotoxic effects in humans. Cervical cancer results from the progression of preinvasive precursor lesions, called low\u2010grade squamous intraepithelial lesions (LGSILs), to high\u2010grade squamous intraepithelial lesions (HGSILs). The gradient from low to high\u2010grade lesions is characterized by increasing nuclear atypia and the failure of cellular differentiation in progressively more superficial levels of the epithelium. These phenotypic changes are hypothesized to be accompanied by increased genetic instability that can be documented using the micronucleus (MN) assay in exfoliated cervical cells. A retrospective study was performed to investigate the frequency of micronucleated cells in cervical smears from women at high risk for developing cervical cancer. Papanicolaou (Pap) smears from 275 women previously studied at a cancer clinic were coded and analyzed for the frequency of micronucleated cells. LGSIL, HGSIL, and invasive carcinoma smears had significantly higher frequencies than normal and ASCUS (abnormal squamous cells of undetermined origin) smears. HGSIL or severely dysplastic smears had the highest frequency of micronucleated cells (although not significantly higher than LGSIL smears), an observation that that could be useful in confirming these types of lesions. The results indicate that the MN frequency in exfoliated cervical cells may be an additional criterion for establishing cervical cancer risk. Environ. Mol. Mutagen. 41:339\u2013343, 2003. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/em.10160","type":"journal-article","created":{"date-parts":[[2003,6,9]],"date-time":"2003-06-09T10:09:20Z","timestamp":1055153360000},"page":"339-343","source":"Crossref","is-referenced-by-count":38,"title":["Positive correlation between the frequency of micronucleated cells and dysplasia in Papanicolaou smears"],"prefix":"10.1002","volume":"41","author":[{"given":"Patricia","family":"Guzm\u00e1n","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rita C.","family":"Sotelo\u2010Regil","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandro","family":"Mohar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda E.","family":"Gonsebatt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,6,6]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(19981225)84:6<375::AID-CNCR10>3.0.CO;2-1"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/255197a0"},{"key":"e_1_2_1_4_1","first-page":"3475","article-title":"Prognostic value of genomic alterations in invasive cervical squamous cell carcinoma of clinical stage IB detected by comparative genomic hybridization","volume":"59","author":"Dellas A","year":"1999","journal-title":"Cancer Res"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.170093297"},{"key":"e_1_2_1_6_1","first-page":"2356","article-title":"Human papillomavirus type 16 E7 oncoprotein\u2010induced abnormal centrosome synthesis is an early event in the evolving malignant phenotype","volume":"61","author":"Duensing S","year":"2001","journal-title":"Cancer Res"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1383-5742(99)00053-8"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1054\/bjoc.1999.0937"},{"key":"e_1_2_1_9_1","series-title":"Biomonitors and biomarkers as indicators of environmental change","first-page":"317","author":"Gonsebatt ME","year":"2000"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.1.479"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2264(199708)19:4<233::AID-GCC5>3.0.CO;2-Y"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199802123380703"},{"key":"e_1_2_1_13_1","series-title":"Monograph on the evaluation of carcinogenic risks to humans","volume-title":"Human papillomavirus","author":"IARC","year":"1995"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2264(199902)24:2<144::AID-GCC7>3.0.CO;2-9"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/91.11.954"},{"key":"e_1_2_1_16_1","first-page":"3230","article-title":"A mutator phenotype in cancer","volume":"61","author":"Loeb LA","year":"2001","journal-title":"Cancer Res"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004280000234"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(20000501)86:3<440::AID-IJC22>3.0.CO;2-A"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.3109\/07357900009012198"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/85.12.958"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19951115)76:10 <1888::AID-CNCR2820761305>3.0.CO;2-H"},{"key":"e_1_2_1_22_1","first-page":"4210","article-title":"Basal cell tetrasomy in low\u2010grade cervical squamous intraepithelial lesions infected with high\u2010risk human papillomavirus","volume":"57","author":"Southern S","year":"1997","journal-title":"Cancer Res"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1161(92)90033-I"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-230X(08)60102-5"}],"container-title":["Environmental and Molecular Mutagenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fem.10160","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/em.10160","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,18]],"date-time":"2023-11-18T01:30:38Z","timestamp":1700271038000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/em.10160"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,1]]},"references-count":23,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2003,1]]}},"alternative-id":["10.1002\/em.10160"],"URL":"https:\/\/doi.org\/10.1002\/em.10160","archive":["Portico"],"relation":{},"ISSN":["0893-6692","1098-2280"],"issn-type":[{"value":"0893-6692","type":"print"},{"value":"1098-2280","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,1]]}}}