{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T22:03:31Z","timestamp":1765231411494},"reference-count":67,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2001,4,4]],"date-time":"2001-04-04T00:00:00Z","timestamp":986342400000},"content-version":"vor","delay-in-days":93,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Environ and Mol Mutagen"],"published-print":{"date-parts":[[2001,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Accumulation of genetic damage in long\u2010lived cell populations with proliferative capacity is implicated in tumorigenesis. Hematopoietic stem cells (hsc) maintain lifetime hematopoiesis, and recent studies demonstrate that hsc in leukemic patients are cytogenetically aberrant. We postulated that exposure to agents associated with increased leukemia risk would induce genomic changes in cells in the hsc compartment. Aneusomy involving chromosomes 2 and 11 in sorted hsc (Lin<jats:sup>\u2212<\/jats:sup>c\u2010kit<jats:sup>+<\/jats:sup>Sca\u20101<jats:sup>+<\/jats:sup>) and maturing lymphoid and myeloid cells from mice that received topical doses of benzene (bz) or trichloroethylene (TCE) was quantified using fluorescence in situ hybridization. Six days after bz or TCE exposure, aneuploid cells in the hsc compartment increase four\u2010 to eightfold in a dose\u2010 and schedule\u2010independent manner. Aneuploid lymphoid and myeloid cells from bz\u2010 and TCE\u2010treated mice approximate controls, except after repeated benzene exposures. Aneuploid cells are more frequent in the hsc compartment than in mature hematopoietic subpopulations. Hematotoxicity was also quantified in bz\u2010 and TCE\u2010exposed hematopoietic subpopulations using two colony\u2010forming assays: CFU\u2010GM (colony\u2010forming units\/granulocyte\u2010macrophage progenitors) and CAFC (cobblestone area\u2013forming cells). Data indicate that bz is transiently cytotoxic (\u22641 week) to hsc subpopulations, and induces more persistent toxicity (&gt;2 weeks) in maturing, committed progenitor subpopulations. TCE is not hematotoxic at the doses applied. In conclusion, we provide direct evidence for induction of aneuploidy in cells in the hsc compartment by topical exposure to bz and TCE. Disruption of genomic integrity and\/or toxicity in hsc subpopulations may be one step in leukemic progression. Environ. Mol. Mutagen. 37:185\u2013194, 2001. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/em.1027","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T21:24:42Z","timestamp":1030310682000},"page":"185-194","source":"Crossref","is-referenced-by-count":5,"title":["Dermal benzene and trichloroethylene induce aneuploidy in immature hematopoietic subpopulations in vivo"],"prefix":"10.1002","volume":"37","author":[{"given":"Cynthia R.","family":"Giver","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosemary","family":"Wong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"II","given":"Dan H.","family":"Moore","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria 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