{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T12:32:07Z","timestamp":1774787527198,"version":"3.50.1"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2001,6,29]],"date-time":"2001-06-29T00:00:00Z","timestamp":993772800000},"content-version":"vor","delay-in-days":179,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Environ and Mol Mutagen"],"published-print":{"date-parts":[[2001,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The human skin cancer\u2010prone disease xeroderma pigmentosum variant (XPV) results from a mutation in the human <jats:italic>RAD30<\/jats:italic> gene, which encodes the lesion bypass DNA polymerase \u03b7. XPV cells are characterized by delayed completion of DNA replication and increased mutagenesis following UV\u2010irradiation. Using extracts of an XPV lymphoblast cell line (GM2449C) that has a truncating mutation in the <jats:italic>RAD30<\/jats:italic> gene, we investigated the effect of a (6\u20104) photoproduct and a cyclobutane pyrimidine dimer (CPD), at a unique \u2013TT\u2013 site on either the leading or lagging strand, on plasmid DNA replication. Compared to normal cell extracts, XPV cell extracts have a reduced capacity to carry out complete replication of DNA containing either a (6\u20104) photoproduct or a CPD on the leading strand, whereas there is little difference between the two cell extracts in replication of DNA containing a lesion on the lagging strand. Inhibition of replication in the presence of a (6\u20104) photoproduct is attributed to arrest of nascent DNA strand synthesis at the lesion site; in XPV cell extracts, the proportion of arrested products is increased compared to that of normal cell extracts. These results are consistent with a requirement for functional DNA polymerase \u03b7 in the replication of a double\u2010stranded plasmid containing either a (6\u20104) photoproduct or a CPD, on the leading but not the lagging strand. Environ. Mol. Mutagen. 38:19\u201329, 2001 \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/em.1046","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T21:50:36Z","timestamp":1030312236000},"page":"19-29","source":"Crossref","is-referenced-by-count":11,"title":["A single (6\u20104) photoproduct inhibits plasmid DNA replication in xeroderma pigmentosum variant cell extracts"],"prefix":"10.1002","volume":"38","author":[{"given":"Jiaqin","family":"Yao","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathleen","family":"Dixon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael P.","family":"Carty","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,6,29]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(75)90209-2"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.21.8141"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(94)90273-9"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/22.15.3026"},{"key":"e_1_2_1_6_1","first-page":"2593","article-title":"Defective post\u2010replication repair in xeroderma pigmentosum variant fibroblasts","volume":"50","author":"Boyer JC","year":"1990","journal-title":"Cancer Res"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.13.1.533"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.16.9637"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12538913"},{"key":"e_1_2_1_10_1","first-page":"4393","volume-title":"The metabolic basis of inherited disease","author":"Cleaver JE","year":"1995"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2787(79)90193-X"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.21.13945"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi972460j"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/24.10.1837"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.21.11922"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/23.11.1919"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.12.6876"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.285.5425.263"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.283.5404.1001"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.23.15975"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/35023030"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(81)90297-7"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.21.9685"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.72.1.219"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.5.6.1238"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.6.3388"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(76)90272-2"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/261593a0"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/21447"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.12.3491"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.12.3100"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/147.4.1557"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1999.5906"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.19.1.147"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(79)90089-7"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004380050698"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.26.11975"},{"key":"e_1_2_1_38_1","first-page":"2445","article-title":"Defective bypass replication of a leading strand cyclobutane thymine dimer in xeroderma pigmentosum variant cell extracts","volume":"58","author":"Svoboda DL","year":"1998","journal-title":"Cancer Res"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.16.7744"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.36.21226"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.950180309"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/0921-8777(95)00034-8"},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0921-8777(99)00052-X"},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.17.7810"},{"key":"e_1_2_1_45_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.050491997"},{"key":"e_1_2_1_46_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.4.7.1286"},{"key":"e_1_2_1_47_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.17.2191"},{"key":"e_1_2_1_48_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.1.185-188.2001"}],"container-title":["Environmental and Molecular Mutagenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fem.1046","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/em.1046","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,18]],"date-time":"2023-11-18T10:56:22Z","timestamp":1700304982000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/em.1046"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,1]]},"references-count":47,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2001,1]]}},"alternative-id":["10.1002\/em.1046"],"URL":"https:\/\/doi.org\/10.1002\/em.1046","archive":["Portico"],"relation":{},"ISSN":["0893-6692","1098-2280"],"issn-type":[{"value":"0893-6692","type":"print"},{"value":"1098-2280","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,1]]}}}