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More than 20 years ago, large\u2010scale screens for UV\u2010sensitive mutants of hamster CHO cells isolated \u223c200 mutants, many of which were assigned to the <jats:italic>XPD\/ERCC2<\/jats:italic> complementation group, but the nature of the mutations was not determined. The XPD protein performs not only an essential viability function as a structural component of transcription initiation factor TFIIH, but also an NER function as a 5\u2032 to 3\u2032 DNA helicase within TFIIH that unwinds DNA on the 3\u2032 side of bulky lesions. Alterations in these XPD functions are responsible for three UV\u2010sensitivity genetic disorders that have distinguishable clinical features. In this study, we sequenced six UV\u2010sensitive ICR170\u2010induced Chinese hamster ovary (CHO) cell mutants that previously were assigned to the <jats:italic>XPD<\/jats:italic> complementation group to determine whether they carry frameshift mutations. All six mutants show 3\u2010 to 5\u2010fold increased hypersensitivity to UV irradiation, similar to the <jats:italic>XPD<\/jats:italic> mutant prototype UV5. Even though ICR170 is a strong frameshift mutagen, all six cell lines contain base substitution mutations, five of which are unique among all mutations identified so far in human and rodent cells. The sixth mutation was identical to the R75W mutation previously found in CHO UVL\u20101. The results presented here contribute to a mutation database that should prove useful in structure\u2010function studies of this unique DNA\u2010structure\u2010specific helicase and its complex mutant phenotypes. Environ. Mol. Mutagen. 38:111\u2013117, 2001. Published 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/em.1060","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T21:15:54Z","timestamp":1030310154000},"page":"111-117","source":"Crossref","is-referenced-by-count":3,"title":["Identification of ICR170\u2010induced <i>XPD<\/i> mutations in UV\u2010sensitive CHO cells"],"prefix":"10.1002","volume":"38","author":[{"given":"Robert S.","family":"Tebbs","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edmund P.","family":"Salazar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Larry H.","family":"Thompson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,10,29]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"245","volume-title":"The genetic basis of human cancer","author":"Bootsma 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