{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T07:45:16Z","timestamp":1769586316633,"version":"3.49.0"},"reference-count":50,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2006,5,3]],"date-time":"2006-05-03T00:00:00Z","timestamp":1146614400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Environ and Mol Mutagen"],"published-print":{"date-parts":[[2006,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nonmelanoma skin cancer (NMSC) is the most frequent type of cancer in humans. Exposure to UV radiation is a major risk factor for NMSC, and oxidative DNA damage, caused either by UV radiation itself or by other agents, may be involved in its induction. Increased sensitivity to oxidative damage and an altered DNA repair capacity (DRC) increase the risk of many types of cancer; however, sensitivity to oxidizing agents has not been evaluated for NMSC, and results regarding DRC in NMSC are inconclusive. In the present study, we evaluated DNA damage and repair in leukocytes from 41 NMSC patients and 45 controls. The Comet assay was used to measure basal and H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010induced DNA damage, as well as the DRC, while the cytokinesis\u2010block micronucleus assay was used to measure the basal level of chromosome damage. Although basal DNA damage was higher for the controls than for the patients, this finding was mainly due to sampling more controls in the summer, which was associated with longer comet tails. In contrast, H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010induced DNA damage was significantly higher in cases than in controls, and this parameter was not influenced by the season of the year. The DRC for the H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010induced damage was similar for cases and controls and unrelated to seasonality. Finally, the frequency of binucleated lymphocytes with micronuclei was similar for cases and controls. The results of this study indicate that NMSC patients are distinguished from controls by an increased sensitivity to oxidative DNA damage. Environ. Mol. Mutagen., 2006. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/em.20220","type":"journal-article","created":{"date-parts":[[2006,5,3]],"date-time":"2006-05-03T22:53:07Z","timestamp":1146696787000},"page":"509-517","source":"Crossref","is-referenced-by-count":18,"title":["DNA damage, oxidative mutagen sensitivity, and repair of oxidative DNA damage in nonmelanoma skin cancer patients"],"prefix":"10.1002","volume":"47","author":[{"given":"Andr\u00e9s","family":"Bendesky","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandra","family":"Michel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monserrat","family":"Sordo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emma S.","family":"Calder\u00f3n\u2010Aranda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonor C.","family":"Acosta\u2010Saavedra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana M.","family":"Salazar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nancy","family":"Podoswa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Ostrosky\u2010Wegman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,5,3]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0401141101"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/canjclin.53.5.292"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12504707"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1383-5718(99)00167-9"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgg108"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"422","DOI":"10.2340\/0001555573422425","article-title":"UV\u2010induced alterations in skin and lymphocytes during a one\u2010week holiday in the Canary Islands in May","volume":"73","author":"Bech\u2010Thomsen N","year":"1993","journal-title":"Acta Derm Venereol"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/92.11.874"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2004.04.001"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)01247-3"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.10332"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.146.s61.2.x"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0921-8777(98)00057-3"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1383-5718(02)00249-8"},{"key":"e_1_2_1_15_1","first-page":"25","article-title":"Reduced DNA repair capacity in laryngeal cancer subjects. A comparison of phenotypic and genotypic results","volume":"62","author":"Gajecka M","year":"2005","journal-title":"Adv Otorhinolaryngol"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2005.07.002"},{"key":"e_1_2_1_17_1","first-page":"1513","article-title":"Polymorphisms in DNA repair genes and associations with cancer risk","volume":"11","author":"Goode EL","year":"2002","journal-title":"Cancer Epidemiol Biomarkers Prev"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/em.20186"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910580206"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-0246"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-04-0846"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1067\/mjd.2003.186"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-483X(03)00150-1"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2004.06.038"},{"key":"e_1_2_1_25_1","first-page":"157","article-title":"Occurrence of other cancers among patients with prior basal cell and squamous cell skin cancer","volume":"7","author":"Karagas MR","year":"1998","journal-title":"Cancer Epidemiol Biomarkers Prev"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1383-5718(03)00171-2"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1067\/S0190-9622(03)00918-6"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrrev.2005.10.001"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0921-8777(97)00057-8"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3835(99)00414-0"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.01-0386com"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jncimonographs.a003483"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0215(20010320)95:2<86::AID-IJC1015>3.0.CO;2-B"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00420-004-0527-0"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4632.2004.02222.x"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/95.7.540"},{"key":"e_1_2_1_37_1","unstructured":"Secretar\u00eda del Medio Ambiente del Distrito Federal (SMA) M\u00e9xico.2006. Available athttp:\/\/www.sma.df.gob.mx\/simat\/home.php?cont=bd. [accessed on March 16 2006]."},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgg037"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2004.02.021"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/tcm.1013"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2005.06587.x"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/18.2.139"},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgi180"},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2004.06.059"},{"key":"e_1_2_1_45_1","volume-title":"The Genetic Basis of Human Cancer","author":"Vogelstein B","year":"2002"},{"key":"e_1_2_1_46_1","doi-asserted-by":"publisher","DOI":"10.1080\/09553009414551401"},{"key":"e_1_2_1_47_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.4.1614"},{"key":"e_1_2_1_48_1","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/gei037"},{"key":"e_1_2_1_49_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.327.7418.794"},{"key":"e_1_2_1_50_1","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1158\/0008-5472.349.65.1","article-title":"Cell cycle checkpoints, DNA damage\/repair, and lung cancer risk","volume":"65","author":"Wu X","year":"2005","journal-title":"Cancer Res"},{"key":"e_1_2_1_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.20939"}],"container-title":["Environmental and Molecular Mutagenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fem.20220","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/em.20220","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T12:52:28Z","timestamp":1700052748000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/em.20220"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,5,3]]},"references-count":50,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2006,8]]}},"alternative-id":["10.1002\/em.20220"],"URL":"https:\/\/doi.org\/10.1002\/em.20220","archive":["Portico"],"relation":{},"ISSN":["0893-6692","1098-2280"],"issn-type":[{"value":"0893-6692","type":"print"},{"value":"1098-2280","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,5,3]]}}}