{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T12:14:26Z","timestamp":1778847266192,"version":"3.51.4"},"reference-count":27,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2006,8,31]],"date-time":"2006-08-31T00:00:00Z","timestamp":1156982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Environ and Mol Mutagen"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Fluorescence in situ hybridization (FISH) with a telomeric peptide nucleic acid probe was employed to analyze the induction of incomplete chromosome elements (ICE; i.e., incomplete chromosomes and terminal fragments) by bleomycin (BLM) in two mammalian cell lines. Chinese hamster embryo cells (CHE cell line, average 2<jats:italic>n<\/jats:italic> = 23) and domestic rabbit cells (CPC cell line, average 2<jats:italic>n<\/jats:italic> = 44) were treated with 2.5 \u03bcg\/ml BLM; after 18 hr of incubation, first\u2010division metaphases were stained with the telomeric probe, and ICE and other unstable chromosomal aberrations were scored. BLM induced ICE, dicentrics, and interstitial acentric fragments in CHE cells, but only ICE in CPC cells. About 50% of the metaphases in BLM\u2010treated CHE cells contained one or more pairs of ICE, while only 20% of treated CPC cells contained ICE. Almost 100% of the BLM\u2010induced ICE in both cell lines consisted of pairs formed by an incomplete chromosome and a terminal fragment. Our results confirm that ICE are the most frequent type of unstable chromosomal aberration induced by BLM in mammalian cells. Moreover, the present study shows that an increase in the chromosome number does not necessarily result in an increase in the frequency of BLM\u2010induced ICE. The results also show that the difference in the chromosomal sensitivity to BLM in CHE and CPC cells is due to differences in the absolute frequency but not in the pattern (i.e., type and proportion) of ICE. Environ. Mol. Mutagen., 2006. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/em.20254","type":"journal-article","created":{"date-parts":[[2006,8,31]],"date-time":"2006-08-31T18:50:39Z","timestamp":1157050239000},"page":"674-681","source":"Crossref","is-referenced-by-count":11,"title":["A comparative analysis of bleomycin\u2010induced incomplete chromosome elements in two mammalian cell lines using a telomeric PNA probe"],"prefix":"10.1002","volume":"47","author":[{"given":"Mar\u00eda C. D\u00edaz","family":"Flaqu\u00e9","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martha S.","family":"Bianchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandro D.","family":"Bolz\u00e1n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,8,31]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"2379","article-title":"Damage and repair induced by bleomycin in the domain of human amplified MYC oncogenes","volume":"50","author":"Bianchi NO","year":"1990","journal-title":"Cancer Res"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/095530098142491"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/095530098141843"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/095530000138817"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/09553000110054610"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2004.02.016"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/em.20051"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2004.11.008"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrrev.2005.12.003"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0027-5107(01)00162-2"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1547"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1667\/RR3477.1"},{"key":"e_1_2_1_14_1","first-page":"61","volume-title":"Statistical Tables for Biological, Agricultural and Medical Research","author":"Fisher RA","year":"1957"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/09553000050028968"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/09553000110083951"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr030117g"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/em.2850210309"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(90)90110-P"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(77)80099-5"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/09553000400017416"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.20125"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/251156a0"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1110(91)90022-N"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1383-5718(98)00169-7"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.12.5983"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1110(78)90019-2"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1080\/095530098142068"}],"container-title":["Environmental and Molecular Mutagenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fem.20254","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/em.20254","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T22:55:38Z","timestamp":1696632938000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/em.20254"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,8,31]]},"references-count":27,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2006,12]]}},"alternative-id":["10.1002\/em.20254"],"URL":"https:\/\/doi.org\/10.1002\/em.20254","archive":["Portico"],"relation":{},"ISSN":["0893-6692","1098-2280"],"issn-type":[{"value":"0893-6692","type":"print"},{"value":"1098-2280","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,8,31]]}}}