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The aim of this study was to explore the use of assessing chromosome aberrations (CA) in peripheral blood lymphocytes as a biomarker for genomic instability in high\u2010risk HPV\u2010infected women with high\u2010grade squamous intraepithelial lesions (HGSIL). A total of 120 women were recruited for this study, following cytology\/colposcopy evaluation and HPV DNA detection. The study groups consisted of 30 HPV(+) women with histologically confirmed cervical intraepithelial neoplasia grade 2\/3 and 30 HPV(+) women with carcinoma in situ (CIS). Two control groups, including 30 women HPV(\u2212) and 30 women HPV(+), were recruited among women who were reported as cytology negative. Lymphocyte cell cultures were established for 52 hr, and 100 complete metaphase cells were evaluated per subject for CA analysis. The results show that women with CIS had significantly higher frequencies of both aneuploidy (0.67 \u00b1 0.20 vs. 0.14 \u00b1 0.08, <jats:italic>P<\/jats:italic> = 0.020) and tetraploidy (0.88 \u00b1 0.23 vs. 0.17 \u00b1 0.08, <jats:italic>P<\/jats:italic> = 0.013) in comparison with HPV(\u2212) controls. These findings suggest the usefulness of peripheral blood lymphocytes to detect genomic instability associated with HPV\u2010induced HGSIL. Environ. Mol. Mutagen., 2008. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/em.20418","type":"journal-article","created":{"date-parts":[[2008,8,19]],"date-time":"2008-08-19T16:04:16Z","timestamp":1219161856000},"page":"688-694","source":"Crossref","is-referenced-by-count":6,"title":["Chromosome aberrations in peripheral blood lymphocytes of high\u2010risk HPV\u2010infected women with HGSIL"],"prefix":"10.1002","volume":"49","author":[{"given":"Rosa E.","family":"\u00c1lvarez\u2010Rosero","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jannet","family":"Rodr\u00edguez\u2010Argote","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yexania Y.","family":"Arboleda\u2010Moreno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sulma L.","family":"Mu\u00f1oz\u2010Ben\u00edtez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos H.","family":"Sierra\u2010Torres","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,8,19]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-4608(97)83452-8"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.54.3.163"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cancergencyto.2004.10.001"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gde.2004.02.007"},{"key":"e_1_2_1_6_1","first-page":"7075","article-title":"The human papillomavirus type 16 E6 and E7 oncoproteins independently induce numerical and structural chromosome instability","volume":"23","author":"Duensing S","year":"2002","journal-title":"Cancer Res"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevEukaryotGeneExpr.v13.i1.20"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/3-540-26980-0_18"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1054\/bjoc.1999.0937"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00329173"},{"key":"e_1_2_1_11_1","first-page":"614","article-title":"DNA ploidy analysis in histopathology. 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