{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T22:54:32Z","timestamp":1790636072695,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2011,4,25]],"date-time":"2011-04-25T00:00:00Z","timestamp":1303689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Environ and Mol Mutagen"],"published-print":{"date-parts":[[2011,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Interindividual differences in DNA repair capacity (DRC) represent an important source of variability in genome integrity and thus influence health risk. In the last decade, DRC measurement has attracted attention as a potential biomarker in cancer prediction. Aim of the present exploratory study was to characterize the variability in DNA damage and DRC on 100 healthy individuals and to identify biological, lifestyle, or genetic factors modulating these parameters. The ultimate goal was to obtain reference data from cancer\u2010free population, which may constitute background for further investigations on cancer patients. The endogenous DNA damage was measured as a level of DNA single\u2010strand breaks and DRC, specific for nucleotide excision repair (NER), was evaluated using modified comet assay, following the challenge of peripheral blood mononuclear cells with benzo[a]pyrene diolepoxide. Additionally, genetic polymorphisms in NER genes (<jats:italic>XPA<\/jats:italic>, <jats:italic>XPC<\/jats:italic>, <jats:italic>XPD,<\/jats:italic> and <jats:italic>XPG<\/jats:italic>) were assessed. We have observed a substantial interindividual variability for both examined parameters. DNA damage was significantly affected by gender and alcohol consumption (<jats:italic>P<\/jats:italic> = 0.003 and <jats:italic>P<\/jats:italic> = 0.012, respectively), whereas DRC was associated with family history of cancer (<jats:italic>P<\/jats:italic> = 0.012). The stratification according to common variants in NER genes showed that DNA damage was significantly modulated by the presence of the variant T allele of <jats:italic>XPC<\/jats:italic> Ala499Val polymorphism (<jats:italic>P<\/jats:italic> = 0.01), while DRC was modulated by the presence of the A allele of <jats:italic>XPA<\/jats:italic> G23A polymorphism (<jats:italic>P<\/jats:italic> = 0.048). Our results indicate the range of endogenous DNA single\u2010strand breaks and capacity of NER in healthy volunteers as well as the role of potentially relevant confounders. Environ. Mol. Mutagen. 2011. \u00a9 2011 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/em.20650","type":"journal-article","created":{"date-parts":[[2011,4,25]],"date-time":"2011-04-25T13:42:50Z","timestamp":1303738970000},"page":"511-517","source":"Crossref","is-referenced-by-count":52,"title":["DNA damage and nucleotide excision repair capacity in healthy individuals"],"prefix":"10.1002","volume":"52","author":[{"given":"Jana","family":"Slyskova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessio","family":"Naccarati","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronika","family":"Polakova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Pardini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludmila","family":"Vodickova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rudolf","family":"Stetina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana","family":"Schmuczerova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zdenek","family":"Smerhovsky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludmila","family":"Lipska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavel","family":"Vodicka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,4,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1383-5718(02)00175-4"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0010164"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1383-5742(00)00032-6"},{"key":"e_1_2_8_5_1","doi-asserted-by":"crossref","first-page":"2242","DOI":"10.1158\/1055-9965.2242.13.12","article-title":"Association between the risk for lung adenocarcinoma and a (\u20104) G\u2010to\u2010A polymorphism in the XPA gene","volume":"13","author":"Butkiewicz D","year":"2004","journal-title":"Cancer Epidemiol Biomarkers Prev"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1385\/MB:26:3:249"},{"key":"e_1_2_8_7_1","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1289\/ehp.96104s3465","article-title":"Oxidative damage to DNA: Do we have a reliable biomarker?","volume":"3","author":"Collins AR","year":"1996","journal-title":"Environ Health Perspect 104 Suppl"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2007.05.005"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/gem051"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/gen007"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01408"},{"key":"e_1_2_8_12_1","first-page":"1118","volume-title":"DNA repair and mutagenesis","author":"Friedberg EC","year":"2006"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10565-007-9047-5"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr040483f"},{"key":"e_1_2_8_15_1","first-page":"1513","article-title":"Polymorphisms in DNA repair genes and associations with cancer risk","volume":"11","author":"Goode EL","year":"2002","journal-title":"Cancer Epidemiol Biomarkers Prev"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2008.15"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/91.14.1194"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1080\/10715760500525807"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/gei064"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.20911"},{"issue":"1","key":"e_1_2_8_21_1","first-page":"1","article-title":"Re\u2010evaluation of some organic chemicals, hydrazine and hydrogen peroxide. 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