{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T01:27:40Z","timestamp":1784251660744,"version":"3.55.0"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,3,19]],"date-time":"2012-03-19T00:00:00Z","timestamp":1332115200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Environ and Mol Mutagen"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Chromosomal instability (CIN) is an important hallmark to oncogenesis and can be diagnosed morphologically by the presence of nuclear anomalies such as micronuclei (MN), nucleoplasmic bridges (NPBs), and nuclear buds (NBuds). We have identified additional nuclear anomalies formed under folate\u2010deficient conditions, defined as \u201cfused\u201d nuclei (FUS), \u201ccircular\u201d nuclei (CIR), and \u201chorse\u2010shoe\u201d nuclei (HS) and investigated their suitability for inclusion as additional CIN biomarkers in the lymphocyte cytokinesis\u2010block micronucleus cytome (CBMN\u2010Cyt) assay. Although the morphological appearance of FUS, CIR, and HS suggested an origin from multiple NPB in the fusion region between the two nuclei, the very low frequency of dicentric chromosomes in metaphase spreads from these cultures did not support this model. Fluorescence <jats:italic>in situ<\/jats:italic> hybridization (FISH) analysis of cytokinesis\u2010blocked binucleated (BN) cells with peptide nucleic acid probes for telomeres and centromeres (PNA\u2013FISH) revealed a high proportion of fusion regions contained both centromeric and telomeric DNA. This suggests that folate deficiency may disrupt the process of sister chromatid separation and chromosome segregation during mitosis. It was concluded that the FUS, CIR, and HS morphologies represent promising biomarkers of CIN that are sensitive to folate deficiency, and further validation and investigation of the mechanisms responsible for their formation is warranted. Mol. Mutagen. 2012. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/em.21688","type":"journal-article","created":{"date-parts":[[2012,3,19]],"date-time":"2012-03-19T06:14:23Z","timestamp":1332137663000},"page":"311-323","source":"Crossref","is-referenced-by-count":46,"title":["Folate deficiency is associated with the formation of complex nuclear anomalies in the cytokinesis\u2010block micronucleus cytome assay"],"prefix":"10.1002","volume":"53","author":[{"given":"Caroline F.","family":"Bull","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graham","family":"Mayrhofer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimphy","family":"Zeegers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grace Low Kah","family":"Mun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Prakash","family":"Hande","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael F.","family":"Fenech","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,3,19]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2005.05.012"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2047"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0141-8130(00)00157-4"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0029665108006988"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/geq065"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1882"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00799"},{"key":"e_1_2_6_9_1","first-page":"1089","article-title":"The effect of folic acid deficiency and MTHFR C677T polymorphism on chromosome damage in human lymphocytes in vitro","volume":"10","author":"Crott JW","year":"2001","journal-title":"Cancer Epidemiol Biomarkers Prev"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrgentox.2007.10.020"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.12.14.1491"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0027-5107(01)00079-3"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.77"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0027-5107(02)00086-6"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgi042"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/geq052"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.4.8.1884"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2004.09.002"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1386"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1909"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/geq062"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6828880"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200803042"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/134.1.48"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2006.12.002"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1883"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.74.082803.133219"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.21276"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/18.2.187"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1159\/000167818"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80932-0"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/18.1.81"}],"container-title":["Environmental and Molecular Mutagenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fem.21688","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/em.21688","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T14:09:40Z","timestamp":1697465380000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/em.21688"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,3,19]]},"references-count":31,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2012,5]]}},"alternative-id":["10.1002\/em.21688"],"URL":"https:\/\/doi.org\/10.1002\/em.21688","archive":["Portico"],"relation":{},"ISSN":["0893-6692","1098-2280"],"issn-type":[{"value":"0893-6692","type":"print"},{"value":"1098-2280","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,3,19]]}}}