{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T19:11:22Z","timestamp":1791141082194,"version":"4.1.0"},"reference-count":53,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2012,10,18]],"date-time":"2012-10-18T00:00:00Z","timestamp":1350518400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Environ and Mol Mutagen"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>During translesion synthesis (TLS), low\u2010fidelity polymerases of the Y\u2010family polymerases bypass DNA damages that block the progression of conventional processive DNA polymerases, thereby allowing the completion of DNA replication. Among the TLS polymerases, DNA polymerase eta (pol\u03b7) performs nucleotide incorporation past ultraviolet (UV) photoproducts and is deficient in cancer\u2010prone <jats:italic>xeroderma pigmentosum<\/jats:italic> variant (XPV) syndrome. Upon UV irradiation, the DNA sliding clamp PCNA is monoubiquitylated on its conserved Lys\u2010164. This event is considered to facilitate the TLS process <jats:italic>in vivo<\/jats:italic> since pol\u03b7 preferentially interacts with monoubiquitylated <jats:bold>PCNA<\/jats:bold> through its <jats:bold>ubiquitin\u2010binding<\/jats:bold> domain (UBZ) as well as its <jats:bold>PCNA<\/jats:bold> interacting peptide (PIP)\u2010box. However, recent observations questioned this model. Therefore, in this study, we re\u2010examined the relative contribution of the regulatory UBZ and PIP domains of pol\u03b7 in response to UVC. We show that simultaneous invalidation of both motifs confers sensitivity to UVC, sensitization by low concentrations of caffeine, prolonged inhibition of DNA synthesis and persistent S phase checkpoint activation, all characteristic features of XPV cells. While each domain is essential for efficient accumulation of pol\u03b7 in replication factories, mutational inactivation of UBZ or PIP motif only confers a slight sensitivity to UVC indicating that, although informative, pol\u03b7 focus analysis is not a reliable tool to assess the pol\u03b7's ability to function in TLS in vivo. Taken together, these data indicate that PIP and UBZ motifs are not required for recruitment but for retention of pol\u03b7 at sites of stalled replication forks. We propose that this is a way to ensure that a sufficient amount of the protein is available for its bypass function. Environ. Mol. Mutagen., 2012. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/em.21741","type":"journal-article","created":{"date-parts":[[2012,10,18]],"date-time":"2012-10-18T18:40:13Z","timestamp":1350585613000},"page":"752-765","source":"Crossref","is-referenced-by-count":34,"title":["Regulation of the specialized DNA polymerase eta: Revisiting the biological relevance of its PCNA\u2010 and ubiquitin\u2010binding motifs"],"prefix":"10.1002","volume":"53","author":[{"given":"Emmanuelle","family":"Despras","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"No\u00e9mie","family":"Delrieu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charl\u00e8ne","family":"Garandeau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sana","family":"Ahmed\u2010Seghir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia L.","family":"Kannouche","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,10,18]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01196-07"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0809844105"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1005492107"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(75)90209-2"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature03482"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1120615"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2009.12.039"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.embor.7400901"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601123"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/22.2.233"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00133"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.111.131433"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10577-009-9105-3"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.139"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2450"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/23.6.959"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dnarep.2006.01.008"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddq046"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201008076"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature03485"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dnarep.2008.05.012"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1002262"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M809745200"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature00991"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.187501"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2003.10.009"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(04)00259-X"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dnarep.2011.08.005"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20070902"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.72.1.219"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/261593a0"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.12.3100"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1755"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.05.003"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0802727105"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1876-1623(08)78004-0"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M605121200"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M702162200"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl1102"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601178"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07343"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0812744106"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3289"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq403"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.027730"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1116345109"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M211838200"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/0921-8777(95)00015-C"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600383"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dnarep.2010.08.001"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0910121106"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0812548106"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2011.06.023"}],"container-title":["Environmental and Molecular Mutagenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fem.21741","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/em.21741","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T15:30:30Z","timestamp":1697470230000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/em.21741"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,10,18]]},"references-count":53,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2012,12]]}},"alternative-id":["10.1002\/em.21741"],"URL":"https:\/\/doi.org\/10.1002\/em.21741","archive":["Portico"],"relation":{},"ISSN":["0893-6692","1098-2280"],"issn-type":[{"value":"0893-6692","type":"print"},{"value":"1098-2280","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,10,18]]}}}