{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T19:30:47Z","timestamp":1790623847601,"version":"4.1.0"},"reference-count":58,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,4,26]],"date-time":"2013-04-26T00:00:00Z","timestamp":1366934400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Environ and Mol Mutagen"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:p>Diethylhexyl phthalate (DEHP) is an estrogen\u2010like compound widely used as a commercial plasticizer and present in medical devices, tubing, food containers and packaging. It is considered an endocrine disruptor and studies on experimental animals showed that exposure to DEHP can alter the function of several organs including liver, kidneys, lungs and reproductive system, particularly the developing testes of prenatal and neonatal males. Exposure to DEHP has been proposed as a potential human health hazard. This study assessed the effects of DEHP on folliculogenesis and oocyte maturation using the mouse as the experimental model. Newborn female mice were hypodermically injected with DEHP at doses of 20 and 40 \u03bcg\/kg per body weight following different exposure regimens during the weaning period. We found that DEHP altered both folliculogenesis and oocyte development. In particular, DEHP exposure significantly decreased the number of the primordial follicles at pubertal and adult age by possibly accelerating the rate of follicle recruitment dynamics, reduced and\/or delayed the level of imprinted gene methylation in the oocytes and increased metaphase II spindle abnormalities in oocytes matured <jats:italic>in vitro<\/jats:italic>. Furthermore, the weight of pups and litter size of mothers exposed to DEHP were significantly lower than controls. Finally, the number of primordial follicles appeared significantly reduced also in the F1 offspring at the adult age. These results show that DEHP may have a number of adverse effects on oogenesis, especially when exposure occurs during early postnatal age, arising concerns about the exposure of human female infants and children to this compound. Environ. Mol. Mutagen. 54:354\u2013361, 2013. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/em.21776","type":"journal-article","created":{"date-parts":[[2013,4,26]],"date-time":"2013-04-26T12:13:16Z","timestamp":1366978396000},"page":"354-361","source":"Crossref","is-referenced-by-count":118,"title":["Diethylhexyl phthalate exposure impairs follicular development and affects oocyte maturation in the mouse"],"prefix":"10.1002","volume":"54","author":[{"given":"Xi\u2010Feng","family":"Zhang","sequence":"first","affiliation":[{"name":"Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong Qingdao Agricultural University Qingdao 266109 China"},{"name":"College of Biological and Pharmaceutical Engineering Wuhan Polytechnic University Wuhan 430023 China"},{"name":"Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences University of Science and Technology of China Hefei Anhui 230027 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lian\u2010Jun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong Qingdao Agricultural University Qingdao 266109 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong Qingdao Agricultural University Qingdao 266109 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan\u2010Ni","family":"Feng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong Qingdao Agricultural University Qingdao 266109 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong Qingdao Agricultural University Qingdao 266109 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin\u2010Mei","family":"Ma","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong Qingdao Agricultural University Qingdao 266109 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evanna","family":"Huynh","sequence":"additional","affiliation":[{"name":"Department of Animal and Poultry Science University of Guelph Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing\u2010Hua","family":"Shi","sequence":"additional","affiliation":[{"name":"Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences University of Science and Technology of China Hefei Anhui 230027 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimo","family":"De Felici","sequence":"additional","affiliation":[{"name":"Department of Biomedicine and Prevention University of Rome \u2018Tor Vergata' 00133 Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Shen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong Qingdao Agricultural University Qingdao 266109 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,4,26]]},"reference":[{"key":"e_1_2_6_3_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/15287398909531232"},{"key":"e_1_2_6_3_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0300-483X(85)90015-0"},{"key":"e_1_2_6_3_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0890-6238(03)00014-5"},{"key":"e_1_2_6_3_5_1","first-page":"23","article-title":"Migration of phthalates from plastic products to model solutions","volume":"27","author":"Bosnir J","year":"2003","journal-title":"Coll Antropol"},{"key":"e_1_2_6_3_6_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.1104139"},{"key":"e_1_2_6_3_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gah179"},{"key":"e_1_2_6_3_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0010201"},{"key":"e_1_2_6_3_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-011-0894-z"},{"key":"e_1_2_6_3_10_1","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1002\/bdrb.20147","article-title":"NTP\u2010CERHR expert panel report on the reproductive and developmental toxicity of bisphenol A","volume":"83","author":"Chapin RE","year":"2008","journal-title":"Birth Defects Res (Part B)"},{"key":"e_1_2_6_3_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/biof.5520330207"},{"key":"e_1_2_6_3_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/des069"},{"key":"e_1_2_6_3_13_1","first-page":"31","article-title":"Two generation reproductive and developmental toxicity following subchronic exposure of pubescent male mice to di(2\u2010ethylhexyl)phthalate","volume":"19","author":"Dobrzy\u0144ska MM","year":"2012","journal-title":"Ann Agric Environ Med"},{"key":"e_1_2_6_3_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrgentox.2007.10.014"},{"key":"e_1_2_6_3_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00244-011-9712-9"},{"key":"e_1_2_6_3_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfl035"},{"key":"e_1_2_6_3_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/35066065"},{"key":"e_1_2_6_3_18_1","doi-asserted-by":"publisher","DOI":"10.1265\/jjh.67.22"},{"key":"e_1_2_6_3_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(197004)25:4<745::AID-CNCR2820250402>3.0.CO;2-2"},{"key":"e_1_2_6_3_20_1","doi-asserted-by":"crossref","first-page":"5624","DOI":"10.1158\/0008-5472.CAN-06-0516","article-title":"Developmental exposure to estradiol and bisphenol A increases susceptibility to prostate carcinogenesis and epigenetically regulates phosphodiesterase type 4 variant 4","volume":"66","author":"Ho SM","year":"2006","journal-title":"Cancer"},{"key":"e_1_2_6_3_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(03)00189-1"},{"key":"e_1_2_6_3_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1207854109"},{"key":"e_1_2_6_3_23_1","doi-asserted-by":"crossref","first-page":"1368","DOI":"10.1093\/humrep\/del010","article-title":"Reducing ischaemic damage in rodent ovarian xenografts transplanted into granulation tissue","volume":"6","author":"Israely T","year":"2006","journal-title":"Hum Reprod"},{"key":"e_1_2_6_3_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2012.02.005"},{"key":"e_1_2_6_3_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2006.04.007"},{"key":"e_1_2_6_3_26_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2002-0131"},{"key":"e_1_2_6_3_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2008.10.005"},{"key":"e_1_2_6_3_28_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.108.070599"},{"key":"e_1_2_6_3_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/es011055j"},{"key":"e_1_2_6_3_30_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.11146"},{"key":"e_1_2_6_3_31_1","doi-asserted-by":"publisher","DOI":"10.1159\/000067012"},{"key":"e_1_2_6_3_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrgentox.2007.10.017"},{"key":"e_1_2_6_3_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0007701"},{"key":"e_1_2_6_3_34_1","doi-asserted-by":"publisher","DOI":"10.1006\/taap.2001.9156"},{"key":"e_1_2_6_3_35_1","doi-asserted-by":"publisher","DOI":"10.1080\/10937400903094091"},{"key":"e_1_2_6_3_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2008.07.002"},{"key":"e_1_2_6_3_37_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.95103574"},{"key":"e_1_2_6_3_38_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.108.074815"},{"key":"e_1_2_6_3_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.modgep.2004.12.007"},{"key":"e_1_2_6_3_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/tcm.1032"},{"key":"e_1_2_6_3_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4274(93)90078-C"},{"key":"e_1_2_6_3_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrgentox.2007.10.009"},{"key":"e_1_2_6_3_43_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-09-0392"},{"key":"e_1_2_6_3_44_1","doi-asserted-by":"publisher","DOI":"10.1080\/026520300283487"},{"key":"e_1_2_6_3_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-1218(96)00161-9"},{"key":"e_1_2_6_3_46_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2011-1450"},{"key":"e_1_2_6_3_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrgentox.2007.10.010"},{"key":"e_1_2_6_3_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2005.08.037"},{"key":"e_1_2_6_3_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204341"},{"key":"e_1_2_6_3_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2010.07.008"},{"key":"e_1_2_6_3_51_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2006-0189"},{"key":"e_1_2_6_3_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.beem.2005.09.005"},{"key":"e_1_2_6_3_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2009.07.131"},{"key":"e_1_2_6_3_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrgentox.2007.10.008"},{"key":"e_1_2_6_3_55_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.0030005"},{"key":"e_1_2_6_3_56_1","doi-asserted-by":"publisher","DOI":"10.1080\/15287390600630054"},{"key":"e_1_2_6_3_57_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2006-1193"},{"key":"e_1_2_6_3_58_1","doi-asserted-by":"publisher","DOI":"10.1673\/031.012.4601"},{"key":"e_1_2_6_3_59_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-011-1372-3"}],"container-title":["Environmental and Molecular Mutagenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fem.21776","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/em.21776","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,18]],"date-time":"2023-10-18T22:35:30Z","timestamp":1697668530000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/em.21776"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,4,26]]},"references-count":58,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2013,6]]}},"alternative-id":["10.1002\/em.21776"],"URL":"https:\/\/doi.org\/10.1002\/em.21776","archive":["Portico"],"relation":{},"ISSN":["0893-6692","1098-2280"],"issn-type":[{"value":"0893-6692","type":"print"},{"value":"1098-2280","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,4,26]]}}}