{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T00:45:14Z","timestamp":1774658714818,"version":"3.50.1"},"reference-count":58,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,10,18]],"date-time":"2014-10-18T00:00:00Z","timestamp":1413590400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Environ and Mol Mutagen"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:p>Circulating cell\u2010free DNA (ccf\u2010DNA) and mtDNA (ccf\u2010mtDNA) have often been used as indicators of cell death and tissue damage in acute and chronic disorders, but little is known about changes in ccf\u2010DNA and ccf\u2010mtDNA concentrations following radiation exposure. The aim of the study was to investigate the impact of chronic low\u2010dose radiation exposure on serum ccf\u2010DNA levels and ccf\u2010mtDNA fragments (mtDNA\u201079 and mtDNA\u2010230) of interventional cardiologists working in high\u2010volume cardiac catheterization laboratory to assess their possible role as useful radiation biomarkers. We enrolled 50 interventional cardiologists (26 males; age\u2009=\u200948.4\u2009\u00b1\u200910 years) and 50 age\u2010 and gender\u2010matched unexposed controls (27 males; age\u2009=\u200947.6\u2009\u00b1\u20098.3 years). Quant\u2010iT\u2122 dsDNA High\u2010Sensitivity assay was used to measure circulating ccf\u2010DNA isolated from serum samples. Quantitative analysis of mtDNA fragments was performed by real\u2010time PCR. No significant relationships were found between ccf\u2010DNA and ccf\u2010mtDNA, and age, gender, smoking, or other clinical parameters. Ccf\u2010DNA levels (44.2\u2009\u00b1\u200931.1 vs. 30.6\u2009\u00b1\u200919.2 ng\/ml, <jats:italic>P<\/jats:italic>\u2009=\u20090.013), ccf\u2010mtDNA\u201079 (2.6\u2009\u00b1\u20092.1 vs. 1.1\u2009\u00b1\u20090.8, <jats:italic>P &lt;<\/jats:italic> 0.01), and ccf\u2010mtDNA\u2010230 copies (2.0\u2009\u00b1\u20091.8 vs. 1.04\u2009\u00b1\u20090.9, <jats:italic>P<\/jats:italic>\u2009=\u20090.02) were significantly higher in interventional cardiologists compared with the non\u2010exposed group. In a subset (<jats:italic>n<\/jats:italic>\u2009=\u200915) of interventional cardiologists with a reliable reconstruction of cumulative professional exposure (59.7\u2009\u00b1\u200948.4 mSv; range: 1.4\u2013182 mS), ccf\u2010DNA (53.2\u2009\u00b1\u200941.3 vs. 36.4\u2009\u00b1\u200922.9 and 32.2\u2009\u00b1\u200920.5, <jats:italic>P<\/jats:italic>\u2009=\u20090.08), mtDNA\u201079 (2.4\u2009\u00b1\u20092.1 vs. 2.03\u2009\u00b1\u20091.7 and 1.09\u2009\u00b1\u20090.82, <jats:italic>P<\/jats:italic>\u2009=\u20090.05), and mtDNA\u2010230 (2.0\u2009\u00b1\u20092.2 vs. 1.5\u2009\u00b1\u20091.4 and 1.04\u2009\u00b1\u20090.9, <jats:italic>P<\/jats:italic>\u2009=\u20090.09) tended to be significantly increased in high\u2010exposure subjects compared with both low\u2010exposure interventional cardiologists and controls. Our results provide evidence for a possible role of circulating DNA as a relevant biomarker of cellular damage induced by exposure to chronic low\u2010dose radiation. Environ. Mol. Mutagen. 56:293\u2013300, 2015. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/em.21917","type":"journal-article","created":{"date-parts":[[2014,10,18]],"date-time":"2014-10-18T10:29:27Z","timestamp":1413628167000},"page":"293-300","source":"Crossref","is-referenced-by-count":24,"title":["Increased circulating cell\u2010free <scp>DNA<\/scp> levels and mt<scp>DNA<\/scp> fragments in interventional cardiologists occupationally exposed to low levels of ionizing radiation"],"prefix":"10.1002","volume":"56","author":[{"given":"Andrea","family":"Borghini","sequence":"first","affiliation":[{"name":"Genetics Research Unit CNR Institute of Clinical Physiology Pisa Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonella","family":"Mercuri","sequence":"additional","affiliation":[{"name":"Genetics Research Unit CNR Institute of Clinical Physiology Pisa Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefano","family":"Turchi","sequence":"additional","affiliation":[{"name":"Genetics Research Unit CNR Institute of Clinical Physiology Pisa Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Rosa","family":"Chiesa","sequence":"additional","affiliation":[{"name":"Genetics Research Unit CNR Institute of Clinical Physiology Pisa Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emanuela","family":"Piccaluga","sequence":"additional","affiliation":[{"name":"Department of Cardiology Ospedale \u201cL. Sacco\u201d Milano Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Grazia","family":"Andreassi","sequence":"additional","affiliation":[{"name":"Genetics Research Unit CNR Institute of Clinical Physiology Pisa Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,10,18]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"318","article-title":"Mutant copies of mitochondrial DNA in tissues and plasma of X\u2010rays exposed mice","volume":"50","author":"Abdullaev SA","year":"2010","journal-title":"Radiat Biol Radioecol"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/HP.0b013e3181b08a20"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/09553000701727523"},{"key":"e_1_2_7_5_1","volume-title":"Committee to Assess Health Risks from Exposure to Low Levels of Ionizing Radiation; Nuclear and Radiation Studies Board, Division on Earth and Life Studies, National Research Council of the National Academies. 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