{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T02:27:05Z","timestamp":1783736825370,"version":"3.55.0"},"reference-count":72,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,1,21]],"date-time":"2019-01-21T00:00:00Z","timestamp":1548028800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["26281029"],"award-info":[{"award-number":["26281029"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ and Mol Mutagen"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:p><jats:italic>In vitro<\/jats:italic> studies indicate that DNA polymerase kappa (Pol\u03ba) is able to accurately and efficiently perform DNA synthesis using templates containing various types of DNA damage, including benzo[<jats:italic>a<\/jats:italic>]pyrene (BP)\u2010induced <jats:italic>N<\/jats:italic><jats:sup>2<\/jats:sup>\u2010deoxyguanosine adducts. In this study, we examined sensitivity of inactivated <jats:italic>Polk<\/jats:italic> knock\u2010in (<jats:italic>Polk<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup>) mice to BP carcinogenicity in the colon by administering an oral dose of BP plus dextran sulfate sodium (DSS), an inflammation causing promoter of carcinogenesis. Although colon cancer was successfully induced by BP plus DSS, there was no significant difference in tumor incidence or multiplicity between <jats:italic>Polk<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> and <jats:italic>Polk<\/jats:italic><jats:sup>+\/+<\/jats:sup> mice. Malignant lymphoma was induced in thymus by the treatment only in <jats:italic>Polk<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice, but it lacked statistical significance. Mutant frequencies (MFs) in the <jats:italic>gpt<\/jats:italic> reporter gene were strongly enhanced in colon; almost to the same extent in both types of mice. Micronucleus formation in bone marrow at the high dose of BP and DNA adducts in colon and lung was not significantly different between two types of mice. Surprisingly, however, <jats:italic>Polk<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice exhibited significantly higher MFs in colon and lung than did <jats:italic>Polk<\/jats:italic><jats:sup>+\/+<\/jats:sup> mice when they were treated with DSS alone. The most prominent mutation induced by DSS treatment was G:C to C:G transversion, whose specific MF in proximal colon was 30 times higher in <jats:italic>Polk<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> than in <jats:italic>Polk<\/jats:italic><jats:sup>+\/+<\/jats:sup> mice. DSS alone did not enhance MF at all in <jats:italic>Polk<\/jats:italic><jats:sup>+\/+<\/jats:sup> mice. The results indicate that Pol\u03ba does not suppress BP\u2010induced mutagenesis and carcinogenesis in the colon, but counteracts inflammation\u2010induced mutagenesis in multiple organs. 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