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Groups of mice received intraperitoneal injections of 0.125\u2009mg\/g of BrdU 24\u2009h prior to irradiation with 0.5 Gy of <jats:sup>60<\/jats:sup>Co gamma rays. DNA breaks measured using SCGE were determined at 30\u2009min after exposure to radiation. The kinetics of micronucleated reticulocyte (MN\u2010RET) induction was determined every 8 h after irradiation up to 72\u2009h. The results from both experimental models indicated that low\u2010level BrdU incorporation into DNA increased the sensitivity to 0.5 Gy of radiation, particularly in the S phase. The formation of micronuclei by gamma rays was produced at three different times using two main mechanisms. In the BrdU\u2010substituted cells, the second mechanism was associated with a high cytotoxic effect that was absent in the irradiated BrdU\u2010unsubstituted cells. The third mechanism, in which micronucleus formation was increased in irradiated substituted cells compared with the irradiated nonsubstituted control cells, was also related to an increase in cytotoxicity. Environ. Mol. 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