{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T11:41:41Z","timestamp":1789040501055,"version":"build-2803163510"},"reference-count":52,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,12,5]],"date-time":"2019-12-05T00:00:00Z","timestamp":1575504000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ and Mol Mutagen"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:p>Tricyclazole (8\u2010methyl\u2010[1,2,4]triazolo[3,4\u2010b][1,3]benzothiazole) is a fungicide used globally on rice for treatment of the seasonal rice blast disease. Human exposure to this fungicide can occur via dietary and nondietary routes. In a battery of <jats:italic>in vitro<\/jats:italic> assays, tricyclazole did not induce gene mutations in bacteria (Ames test) or at the <jats:italic>Hprt<\/jats:italic> locus of CHO cells. It was also negative for the induction of micronuclei in human lymphocyte cultures and unscheduled DNA synthesis (UDS) in primary rat hepatocyte. Paradoxically, tricyclazole induced a mutagenic response at the <jats:italic>Tk<\/jats:italic> locus of the mouse lymphoma L5178Ycells (MLA), which occurred equally among small\/large colony phenotypes. Selection of preexisting mutants leading to a false\u2010positive response in the MLA was ruled out in follow\u2010up experiments. <jats:italic>In vivo<\/jats:italic>, tricyclazole was negative in the rat liver UDS assay, mouse bone micronucleus test and a transgenic (MutaMouse) gene mutation assay in glandular stomach, liver, and kidney. Other supporting evidence for the lack of genotoxicity for tricyclazole comes from an <jats:italic>in vivo<\/jats:italic> study for sister chromatid exchanges in Chinese hamsters, and a dominant lethal test in the male germ cells of mice. The combined evidence from the genotoxicity studies together with the evidence from toxicokinetic, carcinogenicity, developmental, and reproductive toxicity studies confirm that mutagenicity does not occur in relevant <jats:italic>in vivo<\/jats:italic> systems. Data were also compared to potential animal and human exposure, mechanistic data on biological targets and data on analogues, confirming adequacy of the available data for hazard identification and risk assessment. Environ. Mol. Mutagen. 61:300\u2013315, 2020. \u00a9 2019 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/em.22344","type":"journal-article","created":{"date-parts":[[2019,10,21]],"date-time":"2019-10-21T15:32:05Z","timestamp":1571671925000},"page":"300-315","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["A critical Assessment of the Genotoxicity Profile of the Fungicide Tricyclazole"],"prefix":"10.1002","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3215-9820","authenticated-orcid":false,"given":"Marco","family":"Corvaro","sequence":"first","affiliation":[{"name":"Dow AgroSciences, Abingdon  Oxfordshire United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. 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