{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T18:11:22Z","timestamp":1790446282584,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,5,4]],"date-time":"2020-05-04T00:00:00Z","timestamp":1588550400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004410","name":"T\u00fcrkiye Bilimsel ve Teknolojik Ara\u015ftirma Kurumu","doi-asserted-by":"publisher","award":["217Z215"],"award-info":[{"award-number":["217Z215"]}],"id":[{"id":"10.13039\/501100004410","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ and Mol Mutagen"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Clothianidin (CHN) is a member of the neonicotinoid group of insecticides. Its oxidative and DNA damage potential for human lung cells are not known. Therefore, the present study was designed to examine the effects of CHN on DNA damage and oxidative stress in human bronchial epithelial cells (BEAS\u20102B) treated with CHN for 24, 72, and 120\u2009hr. Our results indicate that CHN decreased cell viability in a concentration\u2010dependent manner. CHN induced DNA single\u2010strand breaks because alkaline comet parameters such as tail intensity, DNA in the tail, tail moment, and tail length increased. All CHN concentrations also significantly induced the formation of DNA double\u2010strand breaks (DSBs) because it increased phosphorylated H2AX protein foci for all treatment times and p53\u2010binding protein 1 foci for all treatments except for the lowest concentration (0.15\u2009mM) of 120\u2010hr treatment. DNA damage caused by DNA DSBs was not repaired in a 24\u2010hr recovery period. CHN also induced oxidative stress by decreasing reduced glutathione and increasing lipid peroxidation. These results make it necessary to conduct studies about the detailed carcinogenic potential of CHN in humans because it can induce both oxidative and DNA damage.<\/jats:p>","DOI":"10.1002\/em.22376","type":"journal-article","created":{"date-parts":[[2020,4,14]],"date-time":"2020-04-14T08:43:11Z","timestamp":1586853791000},"page":"647-655","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Clothianidin induces <scp>DNA<\/scp> damage and oxidative stress in bronchial epithelial cells"],"prefix":"10.1002","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3552-3819","authenticated-orcid":false,"given":"Z\u00fclal","family":"Atl\u0131 \u015eekero\u011flu","sequence":"first","affiliation":[{"name":"Department of Molecular Biology and Genetics Faculty of Science and Letters, Ordu University  Ordu Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vedat","family":"\u015eekero\u011flu","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics Faculty of Science and Letters, Ordu University  Ordu Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birsen","family":"Ayd\u0131n","sequence":"additional","affiliation":[{"name":"Department of Biology Faculty of Science and Letters, Amasya University  Amasya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seval","family":"Konta\u015f Yedier","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics Faculty of Science and Letters, Ordu University  Ordu Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emre","family":"\u0130lkun","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics Faculty of Science and Letters, Ordu University  Ordu Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,5,4]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"89","volume-title":"New Insights into Toxicity and Drug Testing","author":"Abdelmigid H.A.","year":"2013"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/01480545.2018.1438458"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10565-012-9215-0"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3109\/01480545.2013.776575"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1289\/EHP456"},{"key":"e_1_2_8_7_1","unstructured":"Brendler\u2010SchwaabS.1999a. TI\u2010435: mutagenicity study for the detection of induced forward mutations in the V79\u2010HPRT assay in vitro. Study No. THT\u20100095. First version of Bayer AG Report No. 28851; revised version of Bayer AG Report No. 26437; Takeda Chemical Industries Ltd Report No. DTOX039. Unpublished report submitted to WHO by Sumitomo Chemical Co. Ltd."},{"key":"e_1_2_8_8_1","unstructured":"Brendler\u2010SchwaabS.1999b. TI 435: test on unscheduled DNA synthesis with rat liver cells in vivo. Study No. THT\u20100100. First amendment to Report No. 28850 of June 16 1999 (2001). Bayer AG Report No. 28850A replacing revised version of Bayer AG Report No. 26915 (raw data added); Takeda Chemical Industries Ltd Report No. DTOX040. Unpublished report submitted to WHO by Sumitomo Chemical Co. Ltd."},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/612647"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.5772\/61174"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-13171-z"},{"key":"e_1_2_8_12_1","unstructured":"DurwardR.2000a. TI\u2010435: L5178Y TK+\/\u2212 mouse lymphoma assay. Study no. THT\u20100099. SafePharm Laboratories Ltd report no. 178\/112; Takeda Chemical Industries Ltd report no. DTOX037. Unpublished report submitted to WHO by Sumitomo Chemical Co. Ltd."},{"key":"e_1_2_8_13_1","unstructured":"DurwardR.2000b. TI\u2010435: micronucleus test in the mouse. Study no. THT\u20100098. SafePharm Laboratories Ltd report no. 178\/113; Takeda Chemical Industries Ltd report no. DTOX038. Unpublished report submitted to WHO by Sumitomo Chemical Co. Ltd."},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(59)90090-6"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.3109\/09553002.2010.549535"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2017.10.149"},{"key":"e_1_2_8_17_1","unstructured":"HerboldB.1999. TI 453: Salmonella\/microsome test plate incorporation and preincubation method. Study no. THT\u20100079. First version of Bayer AG report no. 28849; revised version of Bayer AG report no. 26584; Takeda Chemical Industries Ltd report no. DTOX041. Unpublished report submitted to WHO by Sumitomo Chemical Co. Ltd."},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2017.10.010"},{"key":"e_1_2_8_19_1","unstructured":"HonarvarN.2003a. Micronucleus assay in bone marrow cells of the mouse with TI\u2010435. Study no. 80253. RCC\u2010CCR GmbH Germany report no. 802503; Sumitomo Chemical Co. report no. THT\u20100125. U npublished report submitted to WHO by Sumitomo Chemical Co. Ltd."},{"key":"e_1_2_8_20_1","unstructured":"HonarvarN.2003b. Micronucleus assay in bone marrow cells of the mouse with TI\u2010435. Study no. THT\u20100159. RCC\u2010CCR GmbH Germany report no. 803404; Bayer CropScience SA report no. MO\u201003\u2010015745. Unpublished report submitted to WHO by Sumitomo Chemical Co. Ltd."},{"key":"e_1_2_8_21_1","unstructured":"HonarvarN.2003c. In vivo\/in vitro unscheduled DNA synthesis in rat hepatocytes with TI\u2010435. Study no. 802504. RCC\u2010CCR GmbH Germany; Sumitomo Chemical Co. report no. THT\u20100123. Unpublished report submitted to WHO by Sumitomo Chemical Co. Ltd."},{"key":"e_1_2_8_22_1","unstructured":"HonarvarN.2003d. In vivo\/in vitro unscheduled DNA synthesis in rat hepatocytes with TI\u2010435. Study no. THT\u20100161. RCC\u2010CCR GmbH Germany report no. 803405; Bayer AG report no. MO\u201003\u2010015747. Unpublished report submitted to WHO by Sumitomo Chemical Co. Ltd."},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.b14-00359"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature03114"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/B0-44-451924-6\/00069-7"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-019-02511-9"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.2967\/jnumed.109.071357"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.22644"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2017.04.012"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/insects10010021"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfu066"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(79)90738-3"},{"key":"e_1_2_8_33_1","unstructured":"OtsukaM.1990a. Bacterial reverse mutation test of TIR\u2010435. Study no. THT\u20100087. Hita Research Laboratories Chemical Biotesting Center Chemicals Inspection & Testing Institute report no. T\u20102276; Takeda Chemical Industries Ltd report no. DTOX047. Unpublished report submitted to WHO by Sumitomo Chemical Co. Ltd."},{"key":"e_1_2_8_34_1","unstructured":"OtsukaM.1990b. DNA repair test of TIR\u2010435 inBacillus subtilis. Study no. THT\u20100097. Hita Research Laboratories Chemical Biotesting Center Chemicals Inspection & Testing Institute report no. T\u20102278; Takeda Chemical Industries Ltd report no. DTOX048. Unpublished report submitted to WHO by Sumitomo Chemical Co. Ltd."},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.4061\/2010\/920161"},{"key":"e_1_2_8_36_1","unstructured":"PothA.2003a. Gene mutation assay in Chinese hamster V79 cells in vitro (V79\/HPRT) with TI\u2010435. Study no. 802501. RCC\u2010CCR GmbH Germany; Sumitomo Chemical Co. report no. THT\u20100126. Unpublished report submitted to WHO by Sumitomo Chemical Co. Ltd."},{"key":"e_1_2_8_37_1","unstructured":"PothA.2003b. Gene mutation assay in Chinese hamster V79 cells in vitro (V79\/HPRT) with TI\u2010435. Study no. THT\u20100160. RCC\u2010CCR GmbH Germany report no. 803402; Bayer CropScience SA report no. MO\u201003\u2010015743. Unpublished report submitted to WHO by Sumitomo Chemical Co. Ltd."},{"key":"e_1_2_8_38_1","unstructured":"SchulzM.2003a. in vitro chromosome aberration test in Chinese hamster V79 cells with TI\u2010435. Study no. 803403. RCC\u2010CCR GmbH Germany; Sumitomo Chemical Co. report no. THT\u20100158. Unpublished report submitted to WHO by Sumitomo Chemical Co. Ltd."},{"key":"e_1_2_8_39_1","unstructured":"SchulzM.2003b. in vitro chromosome aberration test in Chinese hamster V79 cells with TI\u2010435. Study no. 802502. RCC\u2010CCR GmbH Germany; Sumitomo Chemical Co. report no. THT\u20100124. Unpublished report submitted to WHO by Sumitomo Chemical Co. Ltd."},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.21037\/tlcr.2019.03.05"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(88)90265-0"},{"key":"e_1_2_8_42_1","unstructured":"SokolowskiA.2003. Salmonella typhimurium reverse mutation assay. Study no. THT\u20100162. RCC\u2010CCR GmbH Germany report no. 803401; Bayer CropScience SA report no. MO\u201003\u2010015766. Unpublished report submitted to WHO by Sumitomo Chemical Co. Ltd."},{"key":"e_1_2_8_43_1","unstructured":"Sumitomo Chemical Takeda Agro Company.2006.Discovery and development of a novel insecticide \u2018clothianidin\u2019. Available at:https:\/\/www.sumitomo-chem.co.jp\/english\/rd\/report\/theses\/docs\/20060202_h6t.pdf[]."},{"key":"e_1_2_8_44_1","unstructured":"ThompsonPW.2000. TI\u2010435: reverse mutation assay \u201cAmes test\u201d using salmonella typhimurium andEscherichia coli. Study no. THT\u20100086. SafePharm Laboratories Ltd report no. 178\/110; Takeda Chemical Industries Ltd report no. DTOX035. Unpublished report submitted to WHO by Sumitomo Chemical Co. Ltd."},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pharmtox.45.120403.095930"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-18-0531"},{"key":"e_1_2_8_47_1","unstructured":"WrightNP.2000. TI\u2010435: chromosome aberration test in CHL cells in vitro. Study no. THT\u20100096. SafePharm Laboratories Ltd report no. 178\/111; Takeda Chemical Industries Ltd report no. DTOX036. Unpublished report submitted to WHO by Sumitomo Chemical Co. Ltd."}],"container-title":["Environmental and Molecular Mutagenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fem.22376","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/em.22376","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/em.22376","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/em.22376","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T10:23:19Z","timestamp":1693995799000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/em.22376"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,4]]},"references-count":46,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2020,7]]}},"alternative-id":["10.1002\/em.22376"],"URL":"https:\/\/doi.org\/10.1002\/em.22376","archive":["Portico"],"relation":{},"ISSN":["0893-6692","1098-2280"],"issn-type":[{"value":"0893-6692","type":"print"},{"value":"1098-2280","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,4]]},"assertion":[{"value":"2020-01-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-04-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-05-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}