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Brominated THMs (Br\u2010THMs) are activated to mutagens via glutathione <jats:italic>S<\/jats:italic>\u2010transferase theta 1 (GSTT1); however, iodinated THMs (I\u2010THMs) have never been evaluated for activation by GSTT1. Among the I\u2010THMs, only triiodomethane (iodoform) has been tested previously for mutagenicity in <jats:italic>Salmonella<\/jats:italic> and was positive (in the absence of GSTT1) in three strains (TA98, TA100, and BA13), all of which have error\u2010prone DNA repair (pKM101). We evaluated five I\u2010THMs (chlorodiiodomethane, dichloroiodomethane, dibromoiodomethane, bromochloroiodomethane, and triiodomethane) for mutagenicity in <jats:italic>Salmonella<\/jats:italic> strain RSJ100, which expresses GSTT1, and its homologue TPT100, which does not; neither strain has pKM101. We also evaluated chlorodiiodo\u2010, dichloroiodo\u2010, and dibromoiodo\u2010methanes in strain TA100 +\/\u2212 rat liver S9 mix; TA100 has pKM101. None was mutagenic in any of the strains. The I\u2010THMs were generally more cytotoxic than their brominated and chlorinated analogues but less cytotoxic than analogous trihalonitromethanes tested previously. All five I\u2010THMs showed similar thresholds for cytotoxicity at ~2.5 \u03bcmoles\/plate, possibly due to release of iodine, a well\u2010known antimicrobial. Although none of these I\u2010THMs was activated by GSTT1, iodoform appears to be the only I\u2010THM that is mutagenic in <jats:italic>Salmonella<\/jats:italic>, only in strains deficient in nucleotide excision repair (<jats:italic>uvrB<\/jats:italic>) and having pKM101. Given that only iodoform is mutagenic among the I\u2010THMs and is generally present at low concentrations in drinking water, the I\u2010THMs likely play little role in the mutagenicity of drinking water.<\/jats:p>","DOI":"10.1002\/em.22423","type":"journal-article","created":{"date-parts":[[2021,1,23]],"date-time":"2021-01-23T06:13:06Z","timestamp":1611382386000},"page":"168-176","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Inability of <scp>GSTT1<\/scp> to activate iodinated halomethanes to mutagens in <i>Salmonella<\/i>"],"prefix":"10.1002","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8357-7988","authenticated-orcid":false,"given":"David M.","family":"DeMarini","sequence":"first","affiliation":[{"name":"Biomolecular and Computational Toxicology Division, Center for Computational Toxicology and Exposure, Office of Research and Development U.S. Environmental Protection Agency  Research Triangle Park North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5046-2890","authenticated-orcid":false,"given":"Sarah H.","family":"Warren","sequence":"additional","affiliation":[{"name":"Biomolecular and Computational Toxicology Division, Center for Computational Toxicology and Exposure, Office of Research and Development U.S. Environmental Protection Agency  Research Triangle Park North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4333-760X","authenticated-orcid":false,"given":"Weston J.","family":"Smith","sequence":"additional","affiliation":[{"name":"863 Fox St  Denver Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6207-4513","authenticated-orcid":false,"given":"Susan D.","family":"Richardson","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry University of South Carolina  Columbia South Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7423-3251","authenticated-orcid":false,"given":"Hannah K.","family":"Liberatore","sequence":"additional","affiliation":[{"name":"Chemical Characterization and Exposure Division, Center for Computational Toxicology and Exposure, Office of Research and Development U.S. Environmental Protection Agency  Research Triangle Park North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,2,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jes.2019.10.007"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/es1000193"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/es9914590"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrgentox.2018.04.005"},{"key":"e_1_2_9_6_1","volume-title":"The 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