{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T09:10:17Z","timestamp":1768641017721,"version":"3.49.0"},"reference-count":29,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2021,10,27]],"date-time":"2021-10-27T00:00:00Z","timestamp":1635292800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ and Mol Mutagen"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In order to investigate the possibility that treatment age affects the genotoxic response to ethyl methane sulfonate (EMS) exposure, we dosed <jats:italic>gpt<\/jats:italic>\u2010delta neonatal mice on postnatal days 1\u201328 with 5\u2013100\u2009mg\/kg\/day of EMS and measured micronucleus (MN) induction in peripheral blood and <jats:italic>gpt<\/jats:italic> gene mutation in liver, lung, bone marrow, small intestine, spleen, and kidney. The data were compared to measurements from similarly exposed adult <jats:italic>gpt<\/jats:italic>\u2010delta mice. Our results indicate that the peripheral blood MN frequencies in mice treated as neonates are not substantially different from those measured in mice treated as adults. There were, however, differences in tissue\u2010specific <jats:italic>gpt<\/jats:italic> mutation responses in mice treated with EMS as neonates and adults. Greater mutant frequencies were seen in DNA isolated from kidney of mice treated as neonates, whereas the mutant frequencies in bone marrow, liver, and spleen were greater in the animals treated as adults. Benchmark dose potency ranking indicated that the differences for kidney were significant. Our data indicate that there are differences in EMS\u2010induced genotoxicity between mice treated as adults and neonates; the differences, however, are relatively small.<\/jats:p>","DOI":"10.1002\/em.22465","type":"journal-article","created":{"date-parts":[[2021,10,14]],"date-time":"2021-10-14T12:06:28Z","timestamp":1634213188000},"page":"482-489","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effect of life stage and target tissue on dose\u2013response assessment of ethyl methane sulfonate\u2010induced genotoxicity"],"prefix":"10.1002","volume":"62","author":[{"given":"Roberta A.","family":"Mittelstaedt","sequence":"first","affiliation":[{"name":"Division of Genetic and Molecular Toxicology, National Center for Toxicological Research U.S. Food and Drug Administration  Jefferson Arkansas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Azra","family":"Dad","sequence":"additional","affiliation":[{"name":"Division of Genetic and Molecular Toxicology, National Center for Toxicological Research U.S. Food and Drug Administration  Jefferson Arkansas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mason G.","family":"Pearce","sequence":"additional","affiliation":[{"name":"Division of Genetic and Molecular Toxicology, National Center for Toxicological Research U.S. Food and Drug Administration  Jefferson Arkansas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert H.","family":"Heflich","sequence":"additional","affiliation":[{"name":"Division of Genetic and Molecular Toxicology, National Center for Toxicological Research U.S. Food and Drug Administration  Jefferson Arkansas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuefei","family":"Cao","sequence":"additional","affiliation":[{"name":"Division of Genetic and Molecular Toxicology, National Center for Toxicological Research U.S. Food and Drug Administration  Jefferson Arkansas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,10,27]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s41021-015-0004-x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/em.21854"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.21173"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2009.02.020"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2009.03.016"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrgentox.2009.04.005"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.20.7971"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/em.22151"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/em.10131"},{"key":"e_1_2_8_11_1","volume-title":"Overall evaluations of the carcinogenicity, an updating on IARC monographs","author":"International Agency for Research on Cancer","year":"1987"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0735-0651(90)90003-X"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2009.07.031"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-529-3_5"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2004.11.011"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2009.03.015"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2009.02.005"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2280(1996)28:4<465::AID-EM24>3.0.CO;2-C"},{"key":"e_1_2_8_19_1","unstructured":"RIVM(2013).PROASThttp:\/\/www.rivm.nl"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1110(84)90007-1"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.22.10681"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0027-5107(98)00147-X"},{"key":"e_1_2_8_23_1","unstructured":"USEPA (United States Environmental Protection Agency)(2014).Guidance for applying quantitative data to develop data\u2010derived extrapolation factors for interspecies and intraspecies extrapolation. 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