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Appraisals combining exposure and long\u2010term post\u2010exposure periods appear as an unavoidable approach in pesticide risk assessment, thus allowing a better understanding of the real impact of agrochemicals in non\u2010target organisms. This study aimed to evaluate the progression of genetic damage in somatic and germ tissues of the crayfish <jats:italic>Procambarus clarkii<\/jats:italic>, also seeking for gender\u2010specificities, following exposure (7\u2009days) to penoxsulam (23\u2009\u03bcg\u2009L<jats:sup>\u22121<\/jats:sup>) and a post\u2010exposure (70\u2009days) period. The same approach was applied to the model genotoxicant ethyl methanesulfonate (EMS; 5\u00a0mg\u2009L<jats:sup>\u22121<\/jats:sup>) as a complementary mean to improve knowledge on genotoxicity dynamics (induction vs. recovery). Penoxsulam induced DNA damage in all tested tissues, disclosing tissue\u2010 and gender\u2010specificities, where females showed to be more vulnerable than males in the gills, while males demonstrated higher susceptibility in what concerns internal organs, that is, hepatopancreas and gonad. Crayfish were unable to recover from the DNA damage induced by EMS in gills and hepatopancreas (both genders) as well as in spermatozoa. The genotoxicity in the hepatopancreas was only perceptible in the post\u2010exposure period. Oxidative DNA lesions were identified in hepatopancreas and spermatozoa of EMS\u2010exposed crayfish. The spermatozoa proved to be the most vulnerable cell type. It became clear that the characterization of the genotoxic hazard of a given agent must integrate a complete set of information, addressing different types of DNA damage, tissue\u2010 and gender\u2010specificities, as well as a long\u2010term appraisal of temporal progression of damage.<\/jats:p>","DOI":"10.1002\/em.22501","type":"journal-article","created":{"date-parts":[[2022,8,17]],"date-time":"2022-08-17T09:29:58Z","timestamp":1660728598000},"page":"296-307","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Improving knowledge on genotoxicity dynamics in somatic and germ cells of crayfish (<i>Procambarus clarkii<\/i>)"],"prefix":"10.1002","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2677-5782","authenticated-orcid":false,"given":"Raquel","family":"Mar\u00e7al","sequence":"first","affiliation":[{"name":"Centre for Environmental and Marine Studies (CESAM), Department of Biology University of Aveiro  Aveiro Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6287-190X","authenticated-orcid":false,"given":"Ana Margarida","family":"Marques","sequence":"additional","affiliation":[{"name":"Centre for Environmental and Marine Studies (CESAM), Department of Biology University of Aveiro  Aveiro Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0552-9643","authenticated-orcid":false,"given":"M\u00e1rio","family":"Pacheco","sequence":"additional","affiliation":[{"name":"Centre for Environmental and Marine Studies (CESAM), Department of Biology University of Aveiro  Aveiro Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3238-4462","authenticated-orcid":false,"given":"Sofia","family":"Guilherme","sequence":"additional","affiliation":[{"name":"Centre for Environmental and Marine Studies (CESAM), Department of Biology University of Aveiro  Aveiro Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,8,29]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0305-0491(89)90040-0"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1039\/c1em10190k"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.marenvres.2013.04.008"},{"key":"e_1_2_9_5_1","unstructured":"Amat L. 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