{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T15:49:19Z","timestamp":1765295359818,"version":"3.40.5"},"reference-count":57,"publisher":"Wiley","issue":"8-9","license":[{"start":{"date-parts":[[2022,10,22]],"date-time":"2022-10-22T00:00:00Z","timestamp":1666396800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001503","name":"Indian Council of Agricultural Research","doi-asserted-by":"publisher","award":["Project code\u2010 2.12"],"award-info":[{"award-number":["Project code\u2010 2.12"]}],"id":[{"id":"10.13039\/501100001503","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ and Mol Mutagen"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The DNA methylation events mark a major epigenetic change in the genome, reflecting non\u2010genetic disease developments and varied phenotypes. The water buffalo is a dairy production animal with wide agro\u2010climatic distribution in India. Breed\u2010wise the coat color of water buffalo varies from ash\u2010gray to jet black. A typical pigmentation pattern is found in one of the breeds of North India, Nili Ravi, with variedly distributed white patches. The DNA methylation pattern could potentially reveal the epigenetic factors responsible for the pigmentation patterns. To address this question, the DNA isolated from the skin tissues of Nili Ravi with varied white pigmentation and black Murrah buffaloes was subjected to reduced representation bisulfite sequencing. DNA methylation analysis revealed, 68.44%, 63.39%, and 47.94% of the promoter regions were hypermethylated in Nili Ravi over\u2010white versus Murrah, Nili Ravi under\u2010white versus Murrah, and Nili Ravi under\u2010white versus Nili Ravi over\u2010white, respectively. Major genes identified to be differentially methylated among over\u2010white and under\u2010white skin tissues in Nili Ravi included <jats:italic>TBX2<\/jats:italic>, <jats:italic>SNAI2<\/jats:italic>, <jats:italic>HERC2<\/jats:italic>, and <jats:italic>CITED1<\/jats:italic>. Overall the results have indicated differential methylation patterns to be potentially involved in hyper or hypopigmentation in Nili Ravi and Murrah buffaloes.<\/jats:p>","DOI":"10.1002\/em.22511","type":"journal-article","created":{"date-parts":[[2022,10,14]],"date-time":"2022-10-14T07:45:35Z","timestamp":1665733535000},"page":"408-417","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Identification of differential methylome signatures of white pigmented skin patches in Nili Ravi buffalo of India"],"prefix":"10.1002","volume":"63","author":[{"given":"Ankita","family":"Gurao","sequence":"first","affiliation":[{"name":"ICAR\u2010National Bureau of Animal Genetic Resources  Karnal Haryana India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rashi","family":"Vasisth","sequence":"additional","affiliation":[{"name":"ICAR\u2010National Bureau of Animal Genetic Resources  Karnal Haryana India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ravinder","family":"Singh","sequence":"additional","affiliation":[{"name":"ICAR\u2010National Bureau of Animal Genetic Resources  Karnal Haryana India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahesh S.","family":"Dige","sequence":"additional","affiliation":[{"name":"ICAR\u2010National Bureau of Animal Genetic Resources  Karnal Haryana India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vikas","family":"Vohra","sequence":"additional","affiliation":[{"name":"ICAR\u2010National Dairy Research Institute  Karnal Haryana India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manishi","family":"Mukesh","sequence":"additional","affiliation":[{"name":"ICAR\u2010National Bureau of Animal Genetic Resources  Karnal Haryana India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanjay","family":"Kumar","sequence":"additional","affiliation":[{"name":"ICAR\u2010Central Institute for Research on Buffaloes  Hisar Haryana India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2845-7486","authenticated-orcid":false,"given":"Ranjit S.","family":"Kataria","sequence":"additional","affiliation":[{"name":"ICAR\u2010National Bureau of Animal Genetic Resources  Karnal Haryana India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,10,22]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_10_2_1","DOI":"10.1186\/gb-2012-13-10-r87"},{"doi-asserted-by":"publisher","key":"e_1_2_10_3_1","DOI":"10.1080\/10428194.2016.1272683"},{"doi-asserted-by":"publisher","key":"e_1_2_10_4_1","DOI":"10.1093\/icb\/icaa142"},{"doi-asserted-by":"publisher","key":"e_1_2_10_5_1","DOI":"10.3389\/fgene.2019.00568"},{"doi-asserted-by":"publisher","key":"e_1_2_10_6_1","DOI":"10.1111\/j.1600-0749.2004.00147.x"},{"doi-asserted-by":"publisher","key":"e_1_2_10_7_1","DOI":"10.3168\/jds.S0022-0302(93)77565-7"},{"doi-asserted-by":"publisher","key":"e_1_2_10_8_1","DOI":"10.1038\/srep26570"},{"doi-asserted-by":"publisher","key":"e_1_2_10_9_1","DOI":"10.1016\/j.abb.2014.06.020"},{"doi-asserted-by":"publisher","key":"e_1_2_10_10_1","DOI":"10.1111\/j.2517-6161.1995.tb02031.x"},{"doi-asserted-by":"publisher","key":"e_1_2_10_11_1","DOI":"10.1016\/j.tree.2019.12.008"},{"doi-asserted-by":"publisher","key":"e_1_2_10_12_1","DOI":"10.1111\/ics.12728"},{"doi-asserted-by":"publisher","key":"e_1_2_10_13_1","DOI":"10.1007\/s11010-016-2680-7"},{"issue":"4","key":"e_1_2_10_14_1","first-page":"987","article-title":"Environmental factors affecting milk yield and fertility traits of Simmental cows raised at the Kazova State Farm and phenotypic correlations between these traits","volume":"29","author":"Cilek S.","year":"2005","journal-title":"Turkish Journal of Veterinary and Animal Sciences"},{"doi-asserted-by":"publisher","key":"e_1_2_10_15_1","DOI":"10.56093\/ijans.v91i3.114150"},{"doi-asserted-by":"publisher","key":"e_1_2_10_16_1","DOI":"10.1371\/journal.pgen.1004705"},{"doi-asserted-by":"publisher","key":"e_1_2_10_17_1","DOI":"10.3390\/ijms17071144"},{"doi-asserted-by":"publisher","key":"e_1_2_10_18_1","DOI":"10.1289\/ehp.8700"},{"doi-asserted-by":"publisher","key":"e_1_2_10_19_1","DOI":"10.1371\/journal.pone.0128969"},{"doi-asserted-by":"publisher","key":"e_1_2_10_20_1","DOI":"10.1007\/s00439-007-0460-x"},{"doi-asserted-by":"publisher","key":"e_1_2_10_21_1","DOI":"10.1186\/1471-2156-8-56"},{"doi-asserted-by":"publisher","key":"e_1_2_10_22_1","DOI":"10.1002\/ajmg.a.62075"},{"doi-asserted-by":"publisher","key":"e_1_2_10_23_1","DOI":"10.1016\/j.molcel.2010.05.004"},{"doi-asserted-by":"publisher","key":"e_1_2_10_24_1","DOI":"10.1093\/nar\/gkn923"},{"doi-asserted-by":"publisher","key":"e_1_2_10_25_1","DOI":"10.1038\/nprot.2008.211"},{"doi-asserted-by":"publisher","key":"e_1_2_10_26_1","DOI":"10.1002\/dvdy.21986"},{"doi-asserted-by":"publisher","key":"e_1_2_10_27_1","DOI":"10.1073\/pnas.1816107116"},{"doi-asserted-by":"publisher","key":"e_1_2_10_28_1","DOI":"10.1038\/s41598-021-96896-2"},{"doi-asserted-by":"publisher","key":"e_1_2_10_29_1","DOI":"10.1016\/S0012-1606(98)80005-5"},{"doi-asserted-by":"publisher","key":"e_1_2_10_30_1","DOI":"10.1016\/S0168-9525(98)01636-9"},{"doi-asserted-by":"publisher","key":"e_1_2_10_31_1","DOI":"10.1038\/nrg3230"},{"doi-asserted-by":"publisher","key":"e_1_2_10_32_1","DOI":"10.17582\/journal.jahp\/2020\/8.2.80.88"},{"doi-asserted-by":"publisher","key":"e_1_2_10_33_1","DOI":"10.1126\/science.1220893"},{"doi-asserted-by":"publisher","key":"e_1_2_10_34_1","DOI":"10.1038\/s41598-018-22608-y"},{"doi-asserted-by":"publisher","key":"e_1_2_10_35_1","DOI":"10.1093\/bioinformatics\/btr167"},{"doi-asserted-by":"publisher","key":"e_1_2_10_36_1","DOI":"10.1186\/s12864-021-08175-z"},{"doi-asserted-by":"publisher","key":"e_1_2_10_37_1","DOI":"10.1093\/molbev\/msaa279"},{"doi-asserted-by":"publisher","key":"e_1_2_10_38_1","DOI":"10.1111\/asj.13367"},{"doi-asserted-by":"publisher","key":"e_1_2_10_39_1","DOI":"10.1073\/pnas.76.6.2862"},{"doi-asserted-by":"publisher","key":"e_1_2_10_40_1","DOI":"10.3389\/fvets.2020.570413"},{"doi-asserted-by":"publisher","key":"e_1_2_10_41_1","DOI":"10.1046\/j.1463-5224.1998.00036.x"},{"doi-asserted-by":"publisher","key":"e_1_2_10_42_1","DOI":"10.1038\/15490"},{"doi-asserted-by":"publisher","key":"e_1_2_10_43_1","DOI":"10.1186\/s13100-018-0131-6"},{"doi-asserted-by":"publisher","key":"e_1_2_10_44_1","DOI":"10.1093\/bioinformatics\/btv566"},{"doi-asserted-by":"publisher","key":"e_1_2_10_45_1","DOI":"10.1182\/blood.V100.4.1274.h81602001274_1274_1286"},{"doi-asserted-by":"publisher","key":"e_1_2_10_46_1","DOI":"10.1371\/journal.Pone.0028857"},{"doi-asserted-by":"publisher","key":"e_1_2_10_47_1","DOI":"10.1002\/ajmg.a.20345"},{"doi-asserted-by":"publisher","key":"e_1_2_10_48_1","DOI":"10.1111\/j.1365-2052.2012.02361.x"},{"doi-asserted-by":"publisher","key":"e_1_2_10_49_1","DOI":"10.1111\/ics.12466"},{"doi-asserted-by":"publisher","key":"e_1_2_10_50_1","DOI":"10.1261\/rna.064865.117"},{"unstructured":"Simons A.(2010).A quality control tool for high throughput sequence data. Available from:http:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/","key":"e_1_2_10_51_1"},{"key":"e_1_2_10_52_1","first-page":"235","article-title":"Absorption of solar radiation by the hair of cattle","volume":"34","author":"Stewart R.E.","year":"1953","journal-title":"Agriculture Engineering"},{"volume-title":"Understanding the relationship between HERC2 and OCA2 variants and iris pigmentation genetics. Purdue University. Ph.D. dissertation. West Lafayette","year":"2021","author":"Wallpe C.","key":"e_1_2_10_53_1"},{"doi-asserted-by":"publisher","key":"e_1_2_10_54_1","DOI":"10.1371\/journal.pone.0143142"},{"doi-asserted-by":"publisher","key":"e_1_2_10_55_1","DOI":"10.1074\/jbc.M115.673129"},{"doi-asserted-by":"publisher","key":"e_1_2_10_56_1","DOI":"10.1002\/ajmg.a.36332"},{"doi-asserted-by":"publisher","key":"e_1_2_10_57_1","DOI":"10.1111\/age.12334"},{"doi-asserted-by":"publisher","key":"e_1_2_10_58_1","DOI":"10.5483\/BMBRep.2013.46.7.250"}],"container-title":["Environmental and Molecular Mutagenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/em.22511","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/em.22511","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/em.22511","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T10:48:25Z","timestamp":1692528505000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/em.22511"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,22]]},"references-count":57,"journal-issue":{"issue":"8-9","published-print":{"date-parts":[[2022,12]]}},"alternative-id":["10.1002\/em.22511"],"URL":"https:\/\/doi.org\/10.1002\/em.22511","archive":["Portico"],"relation":{},"ISSN":["0893-6692","1098-2280"],"issn-type":[{"type":"print","value":"0893-6692"},{"type":"electronic","value":"1098-2280"}],"subject":[],"published":{"date-parts":[[2022,10,22]]},"assertion":[{"value":"2022-06-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}