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In situ bioassays have been carried out using <jats:italic>Cnesterodon decemmaculatus<\/jats:italic> within limnocorrals located in a surface natural water system that receives rainfall excess flowing from an agricultural basin with a typical crop rotation, including corn, wheat and soy. Specimens were taken from the limnocorrals 72\u2009h after a probed natural runoff event toward the water body, and the gill cells were used to evaluate the DNA damage (comet assay, CA), catalase enzyme activity (CAT), and lipid peroxidation (LPO). In addition, the physicochemical analysis of the water (pH, temperature) and the presence and concentration of pesticides were carried out. The results showed significant differences on DNA damage and oxidative stress on the gill cells of the exposed fish compared to controls, being the combination of the rain regime and the mixtures of pesticides used in corn and soy more toxic than in wheat. These results highlight the necessity to understand detrimental processes caused by pesticides used in extensive systems of primary production, in order to prevent and minimize diffuse contamination, contributing to environmental recovery and sustainability.<\/jats:p>","DOI":"10.1002\/em.22529","type":"journal-article","created":{"date-parts":[[2023,1,31]],"date-time":"2023-01-31T13:15:03Z","timestamp":1675170903000},"page":"187-197","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["DNA damage and oxidative stress in gill cells of <i>Cnesterodon decemmaculatus<\/i> exposed to pesticides by runoff source in an agricultural basin"],"prefix":"10.1002","volume":"64","author":[{"given":"N\u00e9stor Abel","family":"Pautasso","sequence":"first","affiliation":[{"name":"INTA Paran\u00e1  Oro Verde Entre R\u00edos Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gisela Laura","family":"Poletta","sequence":"additional","affiliation":[{"name":"C\u00e1tedra de Toxicolog\u00eda, Farmacolog\u00eda y Bioqu\u00edmica Legal, Facultad de Bioqu\u00edmica y Ciencias Biol\u00f3gicas, Universidad Nacional del Litoral Ciudad Universitaria Paraje el Pozo S\/N (3000)  Santa Fe Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)  Ciudad Aut\u00f3noma de Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrique Valent\u00edn","family":"Paravani","sequence":"additional","affiliation":[{"name":"Laboratorio de Qu\u00edmica Ambiental, C\u00e1tedra de Qu\u00edmica General e Inorg\u00e1nica, Facultad de Ingenier\u00eda\u2013UNER  Oro Verde Entre R\u00edos Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Carolina","family":"Sasal","sequence":"additional","affiliation":[{"name":"INTA Paran\u00e1  Oro Verde Entre R\u00edos Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Fernanda","family":"Simoniello","sequence":"additional","affiliation":[{"name":"C\u00e1tedra de Toxicolog\u00eda, Farmacolog\u00eda y Bioqu\u00edmica Legal, Facultad de Bioqu\u00edmica y Ciencias Biol\u00f3gicas, Universidad Nacional del Litoral Ciudad Universitaria Paraje el Pozo S\/N (3000)  Santa Fe Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(84)05016-3"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4165(00)00098-2"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2018.07.134"},{"key":"e_1_2_8_5_1","volume-title":"Quick method for the analysis of numerous highly polar pesticides in foods of plant origin via LC\u2010MS\/MS involving Simultaneous Extraction with Methanol (QuPPe\u2010Method)","author":"Anastassiades M.","year":"2015"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2013.06.041"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.techsoc.2013.01.008"},{"key":"e_1_2_8_8_1","volume-title":"AVMA guidelines for the euthanasia of animals: 2013 Edition","author":"AVMA (American Veterinary Medical Association)","year":"2013"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.marenvres.2013.10.008"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxics6010003"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2019.07.042"},{"key":"e_1_2_8_12_1","unstructured":"Brambilla L. 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