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As they are suspected to generate DNA damage and mutagenicity, an understanding of their mode of action could highlight a carcinogenic potential risk in exposed population. In this article, a prospective study on seven oxy\u2010PAHs selected in terms of occurrence in the environment was conducted on mutagenicity, genotoxicity, and cytotoxicity potentials using in vitro assays including Ames test on five strains, kinetic analysis of cytotoxicity and apoptosis, phosphorylation of histone H2AX, and p53 induction assays on human lung cell line BEAS\u20102B. Ames test demonstrated that mutagenicity pattern depended on the oxy\u2010PAH tested. Except for BAQ, all oxy\u2010PAHs tested gave mutagenic effect, in the absence and\/or in the presence of metabolic activation (S9 fraction). At 24\u2009h of exposure, the majority of oxy\u2010PAHs induced \u03b3\u2010H2AX in BEAS\u20102B cells and\/or phosphorylation of p53 at serine 15 and cell death at highest tested concentrations. Although 9,10\u2010AQ and B[<jats:italic>b<\/jats:italic>]FO were mutagenic in bacteria, they failed to induce any of the other genotoxicity biomarkers. In comparison with the benzo[<jats:italic>a<\/jats:italic>]pyrene, all oxy\u2010PAHs were less potent in terms of genotoxic potential at the same concentration. These results highlighted the genotoxic and mutagenic potential of these oxy\u2010PAHs and provide preliminary information concerning their possible mechanism of action for toxicity, contributing to a better evaluation of the real associated health risks for human and environment.<\/jats:p>","DOI":"10.1002\/em.22531","type":"journal-article","created":{"date-parts":[[2023,2,9]],"date-time":"2023-02-09T12:34:05Z","timestamp":1675946045000},"page":"176-186","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["In vitro genotoxicity potential investigation of 7 oxy\u2010PAHs"],"prefix":"10.1002","volume":"64","author":[{"given":"Adeline","family":"Clerg\u00e9","sequence":"first","affiliation":[{"name":"Normandie Univ, UNICAEN, UNIROUEN, ABTE  Caen France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00e9r\u00e9mie","family":"Le Goff","sequence":"additional","affiliation":[{"name":"INSERM U1086 ANTICIPE (Interdisciplinary Research Unit for Cancers Prevention and Treatment)  Caen France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emilie","family":"Brotin","sequence":"additional","affiliation":[{"name":"Normandie Univ, UNICAEN, PLATON Service Unit, ImpedanCELL  Caen France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edwige","family":"Abeillard","sequence":"additional","affiliation":[{"name":"INSERM U1086 ANTICIPE (Interdisciplinary Research Unit for Cancers Prevention and Treatment)  Caen France"},{"name":"Normandie Univ, UNICAEN, PLATON Service Unit, ImpedanCELL  Caen France"},{"name":"Comprehensive Cancer Center Fran\u00e7ois Baclesse, UNICANCER  Caen France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabelle","family":"Vaudorne","sequence":"additional","affiliation":[{"name":"Normandie Univ, UNICAEN, PRISMM Platform, PLATON Service Unit  Caen France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christophe","family":"Denoyelle","sequence":"additional","affiliation":[{"name":"INSERM U1086 ANTICIPE (Interdisciplinary Research Unit for Cancers Prevention and Treatment)  Caen France"},{"name":"Normandie Univ, UNICAEN, PLATON Service Unit, ImpedanCELL  Caen France"},{"name":"Comprehensive Cancer Center Fran\u00e7ois Baclesse, UNICANCER  Caen France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3494-4109","authenticated-orcid":false,"given":"Ludovic","family":"Le Hegarat","sequence":"additional","affiliation":[{"name":"Anses, French Agency for Food, Environmental and Occupational Health and Safety, Fougeres Laboratory Toxicology of Contaminant Unit  Foug\u00e8res France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rapha\u00ebl","family":"Del\u00e9p\u00e9e","sequence":"additional","affiliation":[{"name":"Normandie Univ, UNICAEN, UNIROUEN, ABTE  Caen France"},{"name":"Comprehensive Cancer Center Fran\u00e7ois Baclesse, UNICANCER  Caen France"},{"name":"Normandie Univ, UNICAEN, PRISMM Platform, PLATON Service Unit  Caen France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,2,22]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.373122"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-1661-0_4"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/26.1.200"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/tx9902082"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-1218(96)90044-0"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1218(87)90014-0"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408444.2019.1605333"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2020.128079"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-014-2586-4"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00412-009-0234-4"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-1218(96)90103-2"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrrev.2012.12.002"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012257060-5\/50012-5"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrgentox.2012.03.014"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(78)90152-5"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9071656"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/tx300476m"},{"key":"e_1_2_6_19_1","unstructured":"International Agency for Research on Cancer. 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