{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T08:22:49Z","timestamp":1783498969585,"version":"3.55.0"},"reference-count":23,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,4,24]],"date-time":"2023-04-24T00:00:00Z","timestamp":1682294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ and Mol Mutagen"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Male B6C3F1 mice were administered styrene monomer by oral gavage for 29 consecutive days at dose levels of 0, 75, 150, or 300\u2009mg\/kg\/day. The highest dose level represented the maximum tolerated dose based on findings in a 28\u2010day dose range\u2010finding study, in which the bioavailability of orally administered styrene was also confirmed. The positive control group received ethyl nitrosourea (ENU; 51.7\u2009mg\/kg\/day) on Study Days 1\u20133 and ethyl methanesulfonate (EMS; 150\u2009mg\/kg\/day) on Study Days 27\u201329 by oral gavage. Approximately 3\u2009h following the final dose, blood was collected to assess erythrocyte <jats:italic>Pig\u2010a<\/jats:italic> mutant and micronucleus frequencies. DNA strand breakage was assessed in glandular stomach, duodenum, kidney, liver, and lung tissues using the alkaline comet assay. The %tail DNA for stomach, liver, lung, and kidney in the comet assay among the styrene\u2010treated groups was neither significantly different from the respective vehicle controls nor was there any dose\u2010related increasing trend in any of the tissues; results for duodenum were interpreted to be inconclusive because of technical issues. The <jats:italic>Pig\u2010a<\/jats:italic> and micronucleus frequencies among styrene\u2010treated groups also did not show significant increases relative to the vehicle controls and there was also no evidence for a dose\u2010related increasing trend. Thus, orally administered styrene did not induce DNA damage, mutagenesis, or clastogenesis\/aneugenesis in these Organization of Economic Co\u2010operation and Development test guideline\u2010compliant genotoxicity studies. Data from these studies can contribute to the overall assessment of genotoxic hazard and risk posed to humans potentially exposed to styrene.<\/jats:p>","DOI":"10.1002\/em.22540","type":"journal-article","created":{"date-parts":[[2023,4,12]],"date-time":"2023-04-12T10:35:52Z","timestamp":1681295752000},"page":"282-290","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Genotoxicity evaluation of orally administered styrene monomer in mice using comet, micronucleus, and <i>Pig\u2010a<\/i> endpoints"],"prefix":"10.1002","volume":"64","author":[{"given":"B. Bhaskar","family":"Gollapudi","sequence":"first","affiliation":[{"name":"Toxicology Consulting  Midland Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,4,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/10937404.2019.1633718"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/15287390306386"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/10937400252972162"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/em.22427"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2012.03.796"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrgentox.2014.09.007"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3791\/59955"},{"key":"e_1_2_9_9_1","first-page":"83","volume-title":"Styrene, styrene\u20107,8\u2010oxide, and quinoline","author":"IARC (International Agency for Research on Cancer)","year":"2019"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrgentox.2019.01.007"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/em.22278"},{"key":"e_1_2_9_12_1","unstructured":"NCI (National Cancer Institute). 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