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Although the buccal micronucleus cytome (BMCyt) assay has evaluated cytotoxicity in drug abuse, there has not been an approach that takes into account this pattern of multiple drug use. Therefore, in this study, we evaluate for the first time the cytogenotoxic effects in multidrug users, and its correlation with the amount consumed and years of abuse. This study was conducted on 166 individuals by the BMCyt assay. A total of 83 individuals with a history of multiple licit (alcohol and tobacco) and at least one illicit drug abuse (marijuana, methamphetamines, cocaine, and\/or inhalants), and 83 healthy individuals, non\u2010drug abusers were analyzed. The results showed that drug abusers had higher frequencies of nuclear abnormalities nuclear buds, binucleated cells, pyknotic nuclei (PNs), karyorrhexis (KX), and abnormally condensed chromatin when compared with healthy controls. Moreover, results suggests that the use of licit and illicit drugs is related to cytogenotoxic damage, as was shown by an upward trend in the frequency of nuclear abnormalities identified in groups 1 (alcohol + tobacco + at least one illicit drug) and 2 (tobacco + at least one illicit drug). Furthermore, a positive correlation was found in the different groups, between the years and the amount of consumption of some drugs (alcohol, methamphetamine, and tobacco) with cytotoxicity markers such as KL, KX, and PNs.<\/jats:p>","DOI":"10.1002\/em.22584","type":"journal-article","created":{"date-parts":[[2024,2,14]],"date-time":"2024-02-14T10:18:41Z","timestamp":1707905921000},"page":"84-95","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Cytogenotoxicity effects in addicts with multidrug consumption"],"prefix":"10.1002","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6923-9771","authenticated-orcid":false,"given":"Ana Elizabeth","family":"Gonz\u00e1lez\u2010Santiago","sequence":"first","affiliation":[{"name":"Departamento de Ciencias Biom\u00e9dicas, Divisi\u00f3n de Ciencias de la Salud Centro Universitario de Tonal\u00e1, Universidad de Guadalajara  Tonal\u00e1 Jalisco 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