{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T12:46:47Z","timestamp":1768826807988,"version":"3.49.0"},"reference-count":15,"publisher":"Wiley","issue":"6-7","license":[{"start":{"date-parts":[[2024,7,16]],"date-time":"2024-07-16T00:00:00Z","timestamp":1721088000000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ and Mol Mutagen"],"published-print":{"date-parts":[[2024,7]]},"abstract":"Abstract<\/jats:title>Methyl tertiary<\/jats:italic>\u2010butyl ether (MTBE) is used as a component of motor vehicle fuel to enhance combustion efficiency and to reduce emissions of carbon monoxide and nitrogen oxides. Although MTBE was largely negative in the in vitro and in vivo genotoxicity studies, isolated reports of positive findings along with the observation of tumors in the rat cancer bioassays raised concern for its in vivo mutagenic potential. To investigate this, transgenic male Big Blue Fischer 344 rats were exposed to 0 (negative control), 400, 1000, and 3000\u2009ppm MTBE via whole body inhalation for 28 consecutive days, 6\u2009h\/day. Mutant frequencies (MF) at the cII<\/jats:italic> locus of the transgene in the nasal epithelium (portal of entry tissue), liver (site of primary metabolism), bone marrow (rapidly proliferating tissue), and kidney (tumor target) were analyzed (5 rats\/exposure group) following a 3\u2010day post\u2010exposure manifestation period. MTBE did not induce a mutagenic response in any of the tissues investigated. The adequacy of the experimental conditions to detect induced mutations was confirmed by utilizing tissue samples from animals treated with the known mutagen ethyl nitrosourea. These data provide support to the conclusion that MTBE is not an in vivo mutagen and male rat kidney tumors are not likely the result of a mutagenic mode of action.<\/jats:p>","DOI":"10.1002\/em.22616","type":"journal-article","created":{"date-parts":[[2024,7,16]],"date-time":"2024-07-16T11:49:50Z","timestamp":1721130590000},"page":"222-229","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Mutagenicity evaluation of methyl tertiary<\/i>\u2010 butyl ether in multiple tissues of transgenic rats following whole body inhalation exposure"],"prefix":"10.1002","volume":"65","author":[{"given":"B. Bhaskar","family":"Gollapudi","sequence":"first","affiliation":[{"name":"Toxicology Consulting Midland Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erik K.","family":"Rushton","sequence":"additional","affiliation":[{"name":"LyondellBasell Rotterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,7,16]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-1263(199705)17:1 3.0.CO;2-K"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3109\/10408444.2015.1052367"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2010.06.003"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/10937404.2022.2041516"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.20124"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/em.22535"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfs305"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408440802368642"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfp049"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-212-44023"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408440600569938"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-1263(199705)17:1 3.0.CO;2-8"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00324794"},{"key":"e_1_2_9_15_1","first-page":"334","article-title":"Cytotoxicity and genotoxicity of methyl tert\u2010butyl ether and its metabolite in human leukaemia cells clin","volume":"31","author":"Tang G.","year":"1997","journal-title":"Journal of Preventive Medicine"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/em.21951"}],"container-title":["Environmental and Molecular Mutagenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/em.22616","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,17]],"date-time":"2024-11-17T09:37:13Z","timestamp":1731836233000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/em.22616"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7]]},"references-count":15,"journal-issue":{"issue":"6-7","published-print":{"date-parts":[[2024,7]]}},"alternative-id":["10.1002\/em.22616"],"URL":"https:\/\/doi.org\/10.1002\/em.22616","archive":["Portico"],"relation":{},"ISSN":["0893-6692","1098-2280"],"issn-type":[{"value":"0893-6692","type":"print"},{"value":"1098-2280","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7]]},"assertion":[{"value":"2024-06-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}