{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T14:59:44Z","timestamp":1747148384858,"version":"3.40.5"},"reference-count":16,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T00:00:00Z","timestamp":1726099200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["AMR\/45\/2011\u2010ECD\u2010I"],"award-info":[{"award-number":["AMR\/45\/2011\u2010ECD\u2010I"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ and Mol Mutagen"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Reporting any uncommon or untapped changes in bacterial genetics or physiology would be of great importance to support the drug development process. We studied 120 <jats:italic>Mycobacterium tuberculosis<\/jats:italic> clinical isolates with different geographical origin within India and their resistance profile and found a significant number of isolates (109) harboring the polymorphism at nucleotide positions 61 and 284 of the <jats:italic>gyrA<\/jats:italic> gene. Bioinformatics analysis of these changes for drug binding suggested no significant change in the binding of the drug but have lower binding energies as compared with the wild\u2010type proteins. Although functionally silent for the <jats:italic>gyrA<\/jats:italic> gene, these changes are indicating a silent geographical and evolutionary change that needs to be further studied for drug discovery and bacterial fitness.<\/jats:p>","DOI":"10.1002\/em.22629","type":"journal-article","created":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T10:56:19Z","timestamp":1726138579000},"page":"289-293","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["High frequency of silent mutations in <i>gyrA<\/i> gene of <i>Mycobacterium tuberculosis<\/i> in Indian isolates"],"prefix":"10.1002","volume":"65","author":[{"given":"Anamika","family":"Gupta","sequence":"first","affiliation":[{"name":"Division of Molecular Biology ICMR\u2010National Institute of Translational Virology and AIDS Research (formerly: National AIDS Research Institute)  Pune Maharashtra India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sudhir K.","family":"Pal","sequence":"additional","affiliation":[{"name":"Division of Molecular Biology ICMR\u2010National Institute of Translational Virology and AIDS Research (formerly: National AIDS Research Institute)  Pune Maharashtra India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6420-9397","authenticated-orcid":false,"given":"Vijay","family":"Nema","sequence":"additional","affiliation":[{"name":"Division of Molecular Biology ICMR\u2010National Institute of Translational Virology and AIDS Research (formerly: National AIDS Research Institute)  Pune Maharashtra India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,9,12]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msw035"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2024.1384791"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0120470"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444902003451"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1758-2946-1-15"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.052548299"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02271-10"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.05286-11"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/18.6.886"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12941-017-0234-9"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.4103\/ijmr.IJMR_493_18"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.22570"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00920-10"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1000002"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.4.773"},{"volume-title":"Global tuberculosis report","year":"2020","author":"World Health Organization","key":"e_1_2_10_17_1"}],"container-title":["Environmental and Molecular Mutagenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/em.22629","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T07:32:08Z","timestamp":1729582328000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/em.22629"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,12]]},"references-count":16,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2024,10]]}},"alternative-id":["10.1002\/em.22629"],"URL":"https:\/\/doi.org\/10.1002\/em.22629","archive":["Portico"],"relation":{},"ISSN":["0893-6692","1098-2280"],"issn-type":[{"type":"print","value":"0893-6692"},{"type":"electronic","value":"1098-2280"}],"subject":[],"published":{"date-parts":[[2024,9,12]]},"assertion":[{"value":"2024-07-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}