{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T14:59:46Z","timestamp":1747148386250,"version":"3.40.5"},"reference-count":52,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,10,12]],"date-time":"2024-10-12T00:00:00Z","timestamp":1728691200000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environ and Mol Mutagen"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Inhalation of nanosized metal oxides may occur at the workplace. Thus, information on potential hazardous effects is needed for risk assessment. We report an investigation of the genotoxic potential of different metal oxide nanomaterials. Acellular and intracellular reactive oxygen species (ROS) production were determined for all the studied nanomaterials. Moreover, mice were exposed by intratracheal instillation to copper oxide (CuO) at 2, 6, and 12\u2009\u03bcg\/mouse, tin oxide (SnO<jats:sub>2<\/jats:sub>) at 54 and 162\u2009\u03bcg\/mouse, aluminum oxide (Al<jats:sub>2<\/jats:sub>O<jats:sub>3<\/jats:sub>) at 18 and 54\u2009\u03bcg\/mouse, zinc oxide (ZnO) at 0.7 and 2\u2009\u03bcg\/mouse, titanium dioxide (TiO<jats:sub>2<\/jats:sub>) and the benchmark carbon black at 162\u2009\u03bcg\/mouse. The doses were selected based on pilot studies. Post\u2010exposure time points were 1 or 28\u2009days. Genotoxicity, assessed as DNA strand breaks by the comet assay, was measured in lung and liver tissue. The acellular and intracellular ROS measurements were fairly consistent. The CuO and the carbon black bench mark particle were potent ROS generators in both assays, followed by TiO<jats:sub>2<\/jats:sub>. Al<jats:sub>2<\/jats:sub>O<jats:sub>3<\/jats:sub>, ZnO, and SnO<jats:sub>2<\/jats:sub> generated low levels of ROS. We detected no increased genotoxicity in this study using occupationally relevant dose levels of metal oxide nanomaterials after pulmonary exposure in mice, except for a slight increase in DNA damage in liver tissue at the highest dose of CuO. The present data add to the body of evidence for risk assessment of these metal oxides.<\/jats:p>","DOI":"10.1002\/em.22634","type":"journal-article","created":{"date-parts":[[2024,10,12]],"date-time":"2024-10-12T08:35:01Z","timestamp":1728722101000},"page":"251-260","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Absence of genotoxicity following pulmonary exposure to metal oxides of copper, tin, aluminum, zinc, and titanium in mice"],"prefix":"10.1002","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2234-6136","authenticated-orcid":false,"given":"Claudia Torero","family":"Gutierrez","sequence":"first","affiliation":[{"name":"National Research Centre for the Working Environment  Copenhagen \u00d8 Denmark"},{"name":"Section of Environmental Health, Department of Public Health University of Copenhagen  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1188-445X","authenticated-orcid":false,"given":"Niels","family":"Hadrup","sequence":"additional","affiliation":[{"name":"National Research Centre for the Working Environment  Copenhagen \u00d8 Denmark"},{"name":"Research group for risk\u2010benefit National Food Institute, Technical University of Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charis","family":"Loizides","sequence":"additional","affiliation":[{"name":"Climate and Atmosphere Research Centre, The Cyprus Institute  Nicosia Cyprus"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iosif","family":"Hafez","sequence":"additional","affiliation":[{"name":"Climate and Atmosphere Research Centre, The Cyprus Institute  Nicosia Cyprus"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Biskos","sequence":"additional","affiliation":[{"name":"Climate and Atmosphere Research Centre, The Cyprus Institute  Nicosia Cyprus"},{"name":"Faculty of Civil Engineering and Geosciences Delft University of Technology  Delft The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7206-0032","authenticated-orcid":false,"given":"Martin","family":"Roursgaard","sequence":"additional","affiliation":[{"name":"Section of Environmental Health, Department of Public Health University of Copenhagen  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7626-709X","authenticated-orcid":false,"given":"Anne Thoustrup","family":"Saber","sequence":"additional","affiliation":[{"name":"National Research Centre for the Working Environment  Copenhagen \u00d8 Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2021-1249","authenticated-orcid":false,"given":"Peter","family":"M\u00f8ller","sequence":"additional","affiliation":[{"name":"Section of Environmental Health, Department of Public Health University of Copenhagen  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6807-1524","authenticated-orcid":false,"given":"Ulla","family":"Vogel","sequence":"additional","affiliation":[{"name":"National Research Centre for the Working Environment  Copenhagen \u00d8 Denmark"},{"name":"National Food Institute, Technical University of Denmark  Kgs. Lyngby Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,10,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.etap.2019.103266"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12989-019-0305-5"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12989-020-00369-9"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0178355"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-8977-9-5"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/15376516.2022.2029656"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/nano11061502"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s42452-019-0592-3"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-022-00754-y"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2019.114830"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2024.105897"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrrev.2022.108441"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfi327"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep15788"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199902113400607"},{"key":"e_1_2_9_17_1","first-page":"504","article-title":"Metal fume fever","volume":"8","author":"Gordon T.","year":"1993","journal-title":"Occupational Medicine"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12989-023-00514-0"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1080\/17435390.2020.1842932"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/gex042"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/17435390.2019.1654004"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/17435390.2023.2221728"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201907476"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S254969"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3109\/08958379509015211"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2013.03.018"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-7843.2010.00644.x"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/get049"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/em.20406"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/em.20300"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2015.08.008"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/em.20629"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/em.21888"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1080\/17435390.2016.1202350"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.3164\/jcbn.17-70"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrgentox.2011.10.011"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12989-017-0238-9"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-020-0398-1"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/gex015"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-020-02923-y"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/ftox.2021.653386"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.7339"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1787\/9789264264885-en"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.3390\/ma8031027"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1080\/17435390.2016.1202351"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-004-0613-9"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1080\/17435390.2022.2152751"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-8977-9-4"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.3109\/17435390.2011.587900"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/s12276-020-0403-3"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021-8502(88)90215-7"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-8977-11-10"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/gew046"}],"container-title":["Environmental and Molecular Mutagenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/em.22634","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T07:31:57Z","timestamp":1729582317000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/em.22634"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10]]},"references-count":52,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2024,10]]}},"alternative-id":["10.1002\/em.22634"],"URL":"https:\/\/doi.org\/10.1002\/em.22634","archive":["Portico"],"relation":{},"ISSN":["0893-6692","1098-2280"],"issn-type":[{"type":"print","value":"0893-6692"},{"type":"electronic","value":"1098-2280"}],"subject":[],"published":{"date-parts":[[2024,10]]},"assertion":[{"value":"2024-06-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}