{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T22:52:38Z","timestamp":1790895158698,"version":"4.1.0"},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,7,14]],"date-time":"2006-07-14T00:00:00Z","timestamp":1152835200000},"content-version":"vor","delay-in-days":4212,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Environ and Mol Mutagen"],"published-print":{"date-parts":[[1995,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The relationship between occupational or environmental exposure to asbestos and the development of mesothelioma, typically after prolonged latency, has been accepted as one of cause and effect. Most studies have concluded that asbestos is not mutagenic to mammalian cells in vitro. We have studied the potential of crocidolite asbestos to induce mutations in a stable mesothelioma cell line, using a mutation assay that measures mutation at the autosomal HLA\u2010A locus and permits clonal growth of mutant cells. The mesothelioma cell line chosen is more akin to the in vivo target cells of asbestos than human peripheral blood lymphocytes used in previous studies. Exposure of mesothelioma cells in culture to both 200 \u03bcg\/ml and 50 \u03bcg\/ml crocidolite for 72 hr did not result in a statistically significant difference in the mutation frequency (MF) in the HLA\u2010A assay when compared to the spontaneous MF in these cells. Mutations in the mesothelioma cells were classified according to their molecular basis. Notwithstanding the lack of statistically significant change in overall MF, molecular analysis of mutants obtained following exposure of mesothelioma cells to crocidolite demonstrated a statistically significant increase in the class of mutations arising from loss of heterozygosity (LOH) events involving the selection locus (HLA\u2010A) and more distal loci. Mutations following exposure to 200 \u03bcg\/ml and 50 \u03bcg\/ml crocidolite showed a greater frequency of LOH than did spontaneous mutants (<jats:italic>P<\/jats:italic> &lt; 0.01 and <jats:italic>P<\/jats:italic> &lt; 0.001, respectively). These results correlate with those obtained in an earlier study using lymphocytes. The mesothelioma cell\u2010based assay may be useful in detecting the mutagenicity of other asbestiform fibers and man\u2010made fibers. \u00a9 1995 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/em.2850260110","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T19:05:17Z","timestamp":1172084717000},"page":"67-71","source":"Crossref","is-referenced-by-count":12,"title":["Loss of heterozygosity in asbestos\u2010induced mutations in a human mesothelioma cell line"],"prefix":"10.1002","volume":"26","author":[{"given":"Katrin","family":"Both","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David R.","family":"Turner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas W.","family":"Henderson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,14]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1161(75)90046-1"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910590417"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3835(83)90071-X"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90186-I"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-6462-1_52"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1985.231"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4684-1363-2_26"},{"key":"e_1_2_1_9_1","first-page":"6305","article-title":"Chrysotile fiber is a strong mutagen in mammalian cells","volume":"52","author":"Hei TK","year":"1992","journal-title":"Cancer Res"},{"key":"e_1_2_1_10_1","first-page":"2170","article-title":"Dependence of asbestos and mineral dust induced transformation of mammalian cells in culture on fiber dimension","volume":"44","author":"Hesterberg TW","year":"1984","journal-title":"Cancer Res"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1218(79)90158-7"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(78)90272-5"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(88)90058-9"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1218(86)90093-5"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.11.3884"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/18.14.4301"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/339055a0"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-7992(89)90118-8"},{"key":"e_1_2_1_19_1","first-page":"4584","article-title":"Molecular nature of in vivo mutations in human cells at the autosomal HLA\u2010A locus","volume":"50","author":"Morley AA","year":"1990","journal-title":"Cancer Res"},{"key":"e_1_2_1_20_1","first-page":"5017","article-title":"Correlation of asbestos induced cytogenetic effects with cell transformation of Syrian hamster embryo cells in culture","volume":"44","author":"Oshimura M","year":"1984","journal-title":"Cancer Res"},{"key":"e_1_2_1_21_1","first-page":"343","article-title":"Interactions between asbestos fibers and metaphase chromosomes of rat pleural mesothelial cells in culture. A scanning and transmission electron microscopic study","volume":"126","author":"Wang NM","year":"1986","journal-title":"Am J Pathol"}],"container-title":["Environmental and Molecular Mutagenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fem.2850260110","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/em.2850260110","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T21:22:49Z","timestamp":1698355369000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/em.2850260110"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,1]]},"references-count":20,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1995,1]]}},"alternative-id":["10.1002\/em.2850260110"],"URL":"https:\/\/doi.org\/10.1002\/em.2850260110","archive":["Portico"],"relation":{},"ISSN":["0893-6692","1098-2280"],"issn-type":[{"value":"0893-6692","type":"print"},{"value":"1098-2280","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,1]]}}}