{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T12:47:37Z","timestamp":1762865257483},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,7,14]],"date-time":"2006-07-14T00:00:00Z","timestamp":1152835200000},"content-version":"vor","delay-in-days":9325,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Environ. mutagen"],"published-print":{"date-parts":[[1981,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Several metals are known mutagens and carcinogens. These metals effectively displace acridine orange from DNA when measured by fluorescence polarization. Displacement of 50% of the acridine orange is obtained with less than 0.5 mM concentrations of lead, manganese, cobalt, zinc, cadmium, nickel, iron, copper, and cis\u2010platinum. In contrast, greater than 80 mM concentrations of lithium, sodium, and potassium are required to displace an equivalent amount of acridine orange from calf thymus DNA. Although cis\u2010platinum shows the best DNA reactivity in this assay, the interaction between this metal and DNA does not occur immediately, as it does for the other metals tested. These results indicate that the acridine orange displacement assay provides a relative measure of the interaction of metals with DNA, and this DNA reactivity shows a positive correlation with mutagenic\/carcinogenic potential.<\/jats:p>","DOI":"10.1002\/em.2860030506","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T21:52:45Z","timestamp":1172008365000},"page":"545-553","source":"Crossref","is-referenced-by-count":13,"title":["Metal mutagens and carcinogens effectively displace acridine orange from DNA as measured by fluorescence polarization"],"prefix":"10.1002","volume":"3","author":[{"given":"Carol L.","family":"Richardson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James","family":"Verna","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gail E.","family":"Schulman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin","family":"Shipp","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ava D.","family":"Grant","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,14]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1218(79)90014-4"},{"key":"e_1_2_1_3_1","first-page":"31","volume-title":"Nucleic Acid\u2010Metal Ion Interactions","author":"Barton JK","year":"1980"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1161(76)90110-2"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2787(70)90509-5"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.571145"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1977.tb07450.x"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(65)80085-7"},{"key":"e_1_2_1_9_1","first-page":"2612","article-title":"Embryonal carcinomas in Syrian hamsters after intratesticular inoculation of zinc chloride during testicular growth","volume":"34","author":"Guthrie J","year":"1974","journal-title":"Cancer Res"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.1.5398.1588"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.910143"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr60273a002"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(79)91611-5"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(61)80004-1"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.49.1.94"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1030640403"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(64)80058-9"},{"key":"e_1_2_1_18_1","first-page":"115","volume-title":"Nucleic Acid Metal Ion Interactions","author":"Loeb LA","year":"1980"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.72.10.3794"},{"key":"e_1_2_1_20_1","first-page":"179","volume-title":"Nucleic Acid\u2010Metal Ion Interactions","author":"Marzilli LG","year":"1980"},{"key":"e_1_2_1_21_1","first-page":"208","article-title":"Inhibition of biological activity of cisplatin by thiourea and L\u2010methionine","volume":"9","author":"Newman AD","year":"1979","journal-title":"J Clin Hematol Oncol"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470186596.ch15"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1039\/tf9565200261"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/51.3.891"},{"key":"e_1_2_1_25_1","first-page":"284","article-title":"Template specificity binding of actinomycin D, nogalamycin, and nogalamycin analogs by competitive fluorescence polarization","volume":"21","author":"Richardson CL","year":"1980","journal-title":"Proc Amer Assoc Cancer Res"},{"key":"e_1_2_1_26_1","first-page":"497","article-title":"Competitive fluorescence polarization study of the binding of 1,4\u2010dihydroxy\u20105,8\u2010bis[[2\u2010[(2\u2010hydroxyethyl)amino]ethyl]amino]9,10\u2010anthracenedione to DNA","volume":"27","author":"Richardson CL","year":"1980","journal-title":"Res Commun Chem Pathol Pharm"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2787(81)90208-2"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3061(00)80121-0"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1006310"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(76)90664-1"},{"key":"e_1_2_1_31_1","first-page":"1744","article-title":"Test for carcinogenicity of metallic compounds by the pulmonary tumor response in strain A mice","volume":"36","author":"Stoner GD","year":"1976","journal-title":"Cancer Res"},{"key":"e_1_2_1_32_1","first-page":"40","article-title":"Carcinogenic effects of metals","volume":"37","author":"Sunderman FW","year":"1978","journal-title":"Fed Proc"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1218(79)90088-0"},{"key":"e_1_2_1_34_1","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1128\/jb.85.5.1003-1010.1963","article-title":"Effects of copper on Bacillus subtilis","volume":"85","author":"Weed LL","year":"1963","journal-title":"J Bact"},{"key":"e_1_2_1_35_1","first-page":"1762","article-title":"Kinetics of formation and disappearance of a DNA cross\u2010linking effect in mouse leukemia L1210 cells treated with cis\u2010 and trans\u2010diamine dichloroplatinum (II)","volume":"38","author":"Zwelling LA","year":"1978","journal-title":"Cancer Res"},{"key":"e_1_2_1_36_1","first-page":"4989","article-title":"Effect of thiourea on survival and DNA\u2010cross\u2010link formation in cells treated with platinum (II) complexes, L\u2010phenylalanine mustard, and bis(2\u2010chloroethyl)\u2010methylamine","volume":"39","author":"Zwelling LA","year":"1979","journal-title":"Cancer Res"}],"container-title":["Environmental Mutagenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fem.2860030506","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/em.2860030506","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T07:36:25Z","timestamp":1695886585000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/em.2860030506"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1981,1]]},"references-count":35,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1981,1]]}},"alternative-id":["10.1002\/em.2860030506"],"URL":"https:\/\/doi.org\/10.1002\/em.2860030506","archive":["Portico"],"relation":{},"ISSN":["0192-2521","1930-238X"],"issn-type":[{"value":"0192-2521","type":"print"},{"value":"1930-238X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1981,1]]}}}