{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T15:27:38Z","timestamp":1770478058282,"version":"3.49.0"},"reference-count":15,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,7,14]],"date-time":"2006-07-14T00:00:00Z","timestamp":1152835200000},"content-version":"vor","delay-in-days":8960,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Environ. mutagen"],"published-print":{"date-parts":[[1982,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The mutagenic response to ultraviolet light and X\u2010irradiation at a number of well characterized independent genetic loci, viz those conferring resistance to 6\u2010thioguanine, ouabain, emetine, methylglyoxal (bis) guanylhydrazone (MGBG), and 5,6\u2010dichlororibofuranosyl benzimidazole (DRB), has been determined. Upon exposure of cells to UV light in the range of 70 to 180 ergs\/mm<jats:sup>2<\/jats:sup>, the frequency of mutants at all of the above genetic loci increased in a linear dose\u2010dependent manner. However, some genetic\u2010locus\u2010specific differences in response to UV light were observed in these studies. In contrast to UV light, exposure of cells to X\u2010rays caused a linear dose\u2010dependent increase in the frequency of mutants at only the thioguanine\u2010resistant (Thg<jats:sup>r<\/jats:sup>) locus, which affects the purine salvage pathway enzyme hypoxanthine\u2010guanine phosphoribosyltransferase, that is not essential for growth under these conditions. The other genetic loci that affect essential cellular functions (and hence should detect only specific base substitution types of mutations), however, showed no increase in the frequencies of mutants upon X\u2010irradiation of cells. These results are in accordance with the nature of the genetic lesions which are caused by these agents and the properties of these genetic markers.<\/jats:p>","DOI":"10.1002\/em.2860040505","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T22:14:05Z","timestamp":1172009645000},"page":"543-551","source":"Crossref","is-referenced-by-count":14,"title":["Mutagenic response to ultraviolet light and X\u2010rays at five independent genetic loci in chinese hamster ovary cells"],"prefix":"10.1002","volume":"4","author":[{"given":"Bhag","family":"Singh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Radhey S.","family":"Gupta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,14]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(71)90125-4"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(72)90140-6"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4899-3103-0_1"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1093\/genetics\/87.1.129","article-title":"Induction of thioguanine\u2010and ouabain\u2010resistant mutants and single strand breaks in the DNA of Chinese hamster ovary cells by 3H\u2010Thymidine","volume":"87","author":"Cleaver JE","year":"1977","journal-title":"Genetics"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(75)90064-0"},{"key":"e_1_2_1_7_1","volume-title":"Handbook of Mutagenicity Test Procedures","author":"Gupta RS","year":"1983"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(80)90181-5"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01538793"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(82)90307-4"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.48.070179.004031"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01538669"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(80)90199-2"},{"key":"e_1_2_1_14_1","first-page":"245","article-title":"A quantitative assay of mutation induction at the hypoxanthine\u2010guanine phosphoribosyltransferase locus in Chinese hamster ovary cells (CHO\/HGPRT system): Utilization with a variety of mutagenic agents","volume":"46","author":"O'Neill JP","year":"1977","journal-title":"Mutat Res"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0027-5107(78)80021-9"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.2307\/3571885"}],"container-title":["Environmental Mutagenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fem.2860040505","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/em.2860040505","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,7]],"date-time":"2023-11-07T02:01:22Z","timestamp":1699322482000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/em.2860040505"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,1]]},"references-count":15,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1982,1]]}},"alternative-id":["10.1002\/em.2860040505"],"URL":"https:\/\/doi.org\/10.1002\/em.2860040505","archive":["Portico"],"relation":{},"ISSN":["0192-2521","1930-238X"],"issn-type":[{"value":"0192-2521","type":"print"},{"value":"1930-238X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1982,1]]}}}