{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T18:29:14Z","timestamp":1764700154297},"reference-count":18,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,7,14]],"date-time":"2006-07-14T00:00:00Z","timestamp":1152835200000},"content-version":"vor","delay-in-days":8230,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Environ. mutagen"],"published-print":{"date-parts":[[1984,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The unscheduled DNA synthesis (UDS) technique has been adapted for use in the chick embryo, in vivo, to determine the relationship between induction of the mixed\u2010function oxidase (MFO) enzyme system and genetic damage from an indirect\u2010acting mutagen\u2010carcinogen. Embryos were injected at 6 days of incubation (DI) with either phenobarbital (PB), a specific inducer of P\u2010450\u2010associated enzyme activities, or 3,4,3\u2032,4\u2032\u2010tetrachlorobiphenyl (TCB), a specific inducer of P<jats:sub>1<\/jats:sub>\u2010450\u2010associated enzyme activities. Aflatoxin B<jats:sub>1<\/jats:sub> (AFB1) was injected 24 hr later (7 DI), followed by a 5\u2010hr continuous <jats:sup>3<\/jats:sup>H\u2010thymidine exposure. The livers were removed, prepared for autoradiography, and hepatocytes were scored for an increase in grains\/nucleus, indicative of UDS. Aflatoxin B<jats:sub>1<\/jats:sub> caused a dose\u2010related increase in UDS in all control and induction groups. Phenobarbital\u2010induced embryos had an increased UDS response while TCB\u2010induced embryos had a decreased UDS response, relative to noninduced embryos, for each dosage of AFB1. This suggests that the genotoxicity of an indirect\u2010acting mutagen\u2010carcinogen can be either increased or decreased, in vivo, depending on the inducer used. The chick embryo provides an excellent system for studying the effect of MFO induction on the genotoxicity of promutagen\u2010carcinogens in a developing system.<\/jats:p>","DOI":"10.1002\/em.2860060106","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T22:17:08Z","timestamp":1172009828000},"page":"41-48","source":"Crossref","is-referenced-by-count":21,"title":["Correlation between mixed\u2010function oxidase enzyme induction and aflatoxin B1\u2010induced unscheduled DNA synthesis in the chick embryo, in vivo"],"prefix":"10.1002","volume":"6","author":[{"given":"Joshua W.","family":"Hamilton","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen E.","family":"Bloom","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,14]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"137","volume-title":"Cytogenetic Assays of Environmental Mutagens","author":"Bloom SE","year":"1982"},{"key":"e_1_2_1_3_1","first-page":"249","volume-title":"Current Progress in Cytogenetics: Sister Chromatid Exchange","author":"Bloom SE","year":"1982"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00284819"},{"key":"e_1_2_1_5_1","first-page":"277","volume-title":"DNA Repair. A Laboratory Manual for Research Procedures","author":"Cleaver JE","year":"1981"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.168.3938.1477"},{"key":"e_1_2_1_7_1","first-page":"296","article-title":"Genetic expression of aflatoxin B1 metabolism: Effects of 3\u2010methylcholanthrene and 2,3,7,8\u2010tetrachlorodibenzo\u2010p\u2010dioxin on the metabolism of aflatoxin B1 and B2 by various inbred strains of mice","volume":"18","author":"Gurtoo HL","year":"1980","journal-title":"Mol Pharmacol"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"3952","DOI":"10.1016\/S0021-9258(17)34783-X","article-title":"Association and dissociation of the Ah locus with the metabolism of aflatoxin B1 by mouse liver","volume":"253","author":"Gurtoo HL","year":"1978","journal-title":"J Biol Chem"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(83)90409-4"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.11.3372"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/1.7.621"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/em.2860040506"},{"key":"e_1_2_1_13_1","first-page":"924","article-title":"Chlorinated biphenyl induction of aryl hydrocarbon hydroxylase activity: A study of the structure activity relationship","volume":"13","author":"Poland A","year":"1977","journal-title":"Mol Pharmacol"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.3109\/00498257609151616"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/em.2860030103"},{"key":"e_1_2_1_16_1","first-page":"214","article-title":"Drug stimulation of \u03b4\u2010aminolevulinic acid synthetase and cytochrome P\u2010450 in vivo in chick embryo liver","volume":"185","author":"Rifkind AB","year":"1977","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.71.12.4955"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/em.2860020402"},{"key":"e_1_2_1_19_1","first-page":"1845","article-title":"Detection of chemical carcinogens by unscheduled DNA synthesis in rat liver primary cell culture","volume":"37","author":"Williams GM","year":"1972","journal-title":"Cancer Res"}],"container-title":["Environmental Mutagenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fem.2860060106","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/em.2860060106","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T21:37:15Z","timestamp":1697837835000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/em.2860060106"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1984,1]]},"references-count":18,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1984,1]]}},"alternative-id":["10.1002\/em.2860060106"],"URL":"https:\/\/doi.org\/10.1002\/em.2860060106","archive":["Portico"],"relation":{},"ISSN":["0192-2521","1930-238X"],"issn-type":[{"value":"0192-2521","type":"print"},{"value":"1930-238X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1984,1]]}}}